Human/Mouse/Rat FKBP25 Antibody

Catalog # Availability Size / Price Qty
AF3955
AF3955-SP
Detection of Human/Mouse/Rat FKBP25 by Western Blot.
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Human/Mouse/Rat FKBP25 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse, and rat FKBP25 in Western blots. In Western blots, this antibody does not cross-react with recombinant human FKBP12, FKBP38, FKBP51, or FKBP52.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FKBP25
Ala2-Asp224
Accession # Q00688
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat FKBP25 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat FKBP25 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, CHP-100 human neuroblastoma cell line, Neuro-2A mouse neuroblastoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP25 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3955) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FKBP25 at approximately 25 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FKBP25

FK506 binding protein, 25 kilodalton molecular weight (FKBP25), also called FKBP3, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans‑proline residues critical for proper folding of proteins. The macrolide immunosuppressant rapamycin is a potent inhibitor of FKBP25. FKBP25 is localized in the nucleus, where it alters the activity of histone deacetylases and transcription factors. FKBP25 is found in many tissues, including the brain, thymus, and spleen.

Long Name
25 kDa FK506 Binding Protein
Entrez Gene IDs
2287 (Human); 30795 (Mouse); 299104 (Rat)
Alternate Names
EC 5.2.1.8,25 kDa FK506-binding protein; FK506 binding protein 3, 25kDa; FK506-binding protein 3 (25kD); FK506-binding protein 3; FKBP25; FKBP25,25 kDa FKBP; FKBP3; FKBP-3; Immunophilin FKBP25; peptidyl-prolyl cis-trans isomerase FKBP3; PPIase FKBP3; PPIase; rapamycin binding protein; Rapamycin-selective 25 kDa immunophilin; rotamase; T-cell

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