Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all FKBP25 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FKBP25
Ala2-Asp224
Accession # Q00688

Specificity

Detects endogenous human, mouse, and rat FKBP25 in Western blots. In Western blots, this antibody does not cross-react with recombinant human FKBP12, FKBP38, FKBP51, or FKBP52.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat FKBP25 Antibody

Detection of Human/Mouse/Rat FKBP25 antibody by Western Blot.

Detection of Human/Mouse/Rat FKBP25 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, CHP-100 human neuroblastoma cell line, Neuro-2A mouse neuroblastoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP25 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3955) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FKBP25 at approximately 25 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Applications for Human/Mouse/Rat FKBP25 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: MCF-7 human breast cancer cell line, CHP-100 human neuroblastoma cell line, Neuro-2A mouse neuroblastoma cell line, and PPC-12 rat adrenal pheochromocytoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FKBP25

FK506 binding protein, 25 kilodalton molecular weight (FKBP25), also called FKBP3, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans‑proline residues critical for proper folding of proteins. The macrolide immunosuppressant rapamycin is a potent inhibitor of FKBP25. FKBP25 is localized in the nucleus, where it alters the activity of histone deacetylases and transcription factors. FKBP25 is found in many tissues, including the brain, thymus, and spleen.

Long Name

25 kDa FK506 Binding Protein

Alternate Names

FKBP3

Entrez Gene IDs

2287 (Human); 30795 (Mouse); 299104 (Rat)

Gene Symbol

FKBP3

UniProt

Q00688

Additional FKBP25 Products

Product Documents for Human/Mouse/Rat FKBP25 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat FKBP25 Antibody

For research use only

Related Research Areas

Phosphatases and Regulators

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