Detection of Human/Mouse/Rat FKBP25 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, CHP-100 human neuroblastoma cell line, Neuro-2A mouse neuroblastoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP25 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3955) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FKBP25 at approximately 25 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
FK506 binding protein, 25 kilodalton molecular weight (FKBP25), also called FKBP3, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans‑proline residues critical for proper folding of proteins. The macrolide immunosuppressant rapamycin is a potent inhibitor of FKBP25. FKBP25 is localized in the nucleus, where it alters the activity of histone deacetylases and transcription factors. FKBP25 is found in many tissues, including the brain, thymus, and spleen.
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