Human/Mouse/Rat GATE-16 Antibody

Catalog # Availability Size / Price Qty
MAB9035
MAB9035-SP
Detection  of Human, Mouse, and Rat GATE-16 by Western Blot.
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Human/Mouse/Rat GATE-16 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat GATE-16 in Western blot.
Source
Monoclonal Rat IgG2B Clone # 853746
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E.coli-derived recombinant human GATE16
Accession # P60520
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Human, Mouse, and Rat GATE-16 by Western Blot. View Larger

Detection of Human, Mouse, and Rat GATE-16 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, mouse brain (cerebellum) tissue, and rat brain (total) tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat GATE-16 Monoclonal Antibody
(Catalog # MAB9035) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for GATE-16 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATE-16

Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), also known as Apg8p2 and GABARAPL2, is a 117 amino acid (aa) polypeptide and a member of the Autophagy-related 8 (Atg8) family of proteins (1). GATE-16/Apg8p2 has 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GATE-16/Apg8p2 is best known for its role in autophagy (2,3). GATE-16/Apg8p2 covalently attaches to phosphatidylethanolamine (PE) the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome formation (4,5). It may also be involved in cargo recruitment to autophagosomes (1).

References
  1. Shpilka, T. et al. (2011) Genome Biol. 12:226.
  2. Wang, H. et al. (1999) Nature 397:69.
  3. Leil, T.A. et al. (2004) J. Neurosci. 24:11429.
  4. Weidberg, H. et al. (2010) EMBO J. 29:1792.
  5. Weidberg, H. et al. (2011) Ann. Rev. Biochem. 80:125
Long Name
Golgi-associated ATPase Enhancer of 16 kDa
Entrez Gene IDs
11345 (Human); 100042211 (Mouse); 64670 (Rat)
Alternate Names
Apg8p2; ATG8; ATG8C; FLC3A; GABARAPL2; gamma-aminobutyric acid receptor-associated protein-like 2; Ganglioside expression factor 2; GATE16; GATE-16; GEF2; GEF-2; GEF2GEF-2; General Protein Transport Factor P16; Golgi-Associated ATPase Enhancer Of 16 KDa; MAP1 light chain 3 related protein; MAP1 light chain 3-related protein

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