Human/Mouse/Rat GRIN2C/NMDAR2C Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Human, mouse, rat ~140 kDa NMDAR NR2C subunit
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Fusion protein from the NR2C subunit of the NMDA Receptor
  • Formulation
    10 μg per vial; lyophilized in 5 mM ammonium bicarbonate.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
  • Immunoprecipitation
    3 μL /200 μg lysate
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of GRIN2C/NMDAR2C by Western Blot. Western blot of 10 μg of rat brain (cerebellum) lysate showing specific immunolabeling of the ~140 kDa NR2C subunit of the NMDA Receptor. The labeling by the antibody was blocked by the N-Terminal NR2C subunit NMDA Receptor fusion protein used as the antigen.
Preparation and Storage
  • Reconstitution
    This antibody should be reconstituted in 50 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    The lyophilized product is stable at ≤ -20° C for at least 1 year. After reconstitution the antibody should be aliquoted and stored at ≤ -20° C.
Background: GRIN2C/NMDAR2C
NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650 - 850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics such as conductance and Mg++ sensitivity. In addition, NR2 subunits mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes that code for NR2 subunits (NR2A-D). The two (or three) NR2 subunits making up the NMDAR may be homodimers or heterodimers. The 2C form generates a low conductance NMDAR. This receptor is particularly abundant in the cerebellum and thalamus. Rat NR2C is a 180 kDa, 1237 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 535 aa extracellular domain (ECD) and a 401 aa cytoplasmic region. The molecule is described as 4-TM. However, the second-TM segment is only partial (or reenterant), and this makes the C-terminus intracellular. The loop connecting TM segments 3 and 4, plus the N-terminal ECD likely constitute the glutamate binding site. Rat NR2C shares 97% and 88% aa sequence identity to mouse and human NR2C, respectively.
  • References:
    1. Stephenson, F.A. (2001) Curr. Drug Targets 2:233.
    2. Cull-candy, S.G. and D.N. Leszkiewicz (2004) Sci. STKE re16.
    3. Prybylowski, K. and R.J. Wenthold (2004) J. Biol. Chem. 279:9673.
    4. Monyer, H. et al. (1992) Science 256:1217.
  • Long Name:
    Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2C
  • Entrez Gene IDs:
    2905 (Human); 14813 (Mouse); 24411 (Rat)
  • Alternate Names:
    glutamate [NMDA] receptor subunit epsilon-3; glutamate receptor, ionotropic, N-methyl D-aspartate 2C; GRIN2C; NMDA R, NR2C Subunit; NMDAR2C; NMDAR2CNR2C; N-methyl D-aspartate receptor subtype 2C; N-methyl-D-aspartate receptor subunit 2C; NR2C
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