Home / GRK2 / Human/Mouse/Rat GRK2 Alexa Fluor® 700-conjugated Antibody

Human/Mouse/Rat GRK2 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB43391N Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB43391N-100UG

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Human/Mouse/Rat GRK2 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human GRK2 in direct ELISAs and Western blots.
Source
Monoclonal Rabbit IgG Clone # 2089B
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human GRK2
Pro468-Leu689
Accession # P25098
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Knockout Validated
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: GRK2

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK2 is a member of the beta ARK subfamily, and was originally termed beta ‑adrenergic receptor kinase 1 ( beta ARK1).

Long Name
G protein-coupled Receptor Kinase 2
Entrez Gene IDs
156 (Human); 110355 (Mouse); 25238 (Rat)
Alternate Names
ADRBK1; adrenergic, beta, receptor kinase 1; BARK; BARK1; BARK1beta-ARK-1; Beta-ARK1; Beta-ARK-1; EC 2.7.11; EC 2.7.11.15; FLJ16718; G-protein coupled receptor kinase 2; GRK2; GRK2beta-adrenergic receptor kinase 1

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