Human/Mouse/Rat GRK2 Antibody

Recombinant Monoclonal Antibody..
  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human GRK2 in direct ELISAs and Western blots.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 2089B
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    E.coli-derived recombinant human GRK2
    Pro468-Leu689
    Accession # P25098
  • Formulation
    Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Simple Western
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat GRK2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line, Ramos human Burkitt's lymphoma cell line, LL/2 mouse Lewis lung carcinoma cell line, H4‑II‑E‑C3 rat hepatoma cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat GRK2 Monoclonal Antibody (Catalog # MAB43391) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for GRK2 at approximately 76 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human GRK2 by Simple WesternTM. Simple Western lane view shows lysates of Ramos human Burkitt's lymphoma cell line and THP‑1 human acute monocytic leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for GRK2 at approximately 80 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse/
Rat GRK2 Monoclonal Antibody (Catalog # MAB43391). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: GRK2

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK2 is a member of the beta ARK subfamily, and was originally termed beta ‑adrenergic receptor kinase 1 ( beta ARK1).

  • Long Name:
    G protein-coupled Receptor Kinase 2
  • Entrez Gene IDs:
    156 (Human); 110355 (Mouse); 25238 (Rat)
  • Alternate Names:
    ADRBK1; adrenergic, beta, receptor kinase 1; BARK; BARK1; BARK1beta-ARK-1; Beta-ARK1; Beta-ARK-1; EC 2.7.11; EC 2.7.11.15; FLJ16718; G-protein coupled receptor kinase 2; GRK2; GRK2beta-adrenergic receptor kinase 1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 26  
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Molecular Weight Markers
Description Application Cat# Citations Images  

Full-Range Molecular Weight Marker, Biotinylated

MW001
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG Horseradish Peroxidase-conjugated Antibody

WB HAF008 12  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 1  
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Donkey Anti-Rabbit IgG NL557 Affinit Purified PAb

Flow, IHC, ICC NL004 6
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 2
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 2  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG Antibody

WB AF008 1
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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