Human/Mouse/Rat GRK2 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB43391-100
MAB43391-SP
Detection  of Human, Mouse, and Rat GRK2 by Western Blot.
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Human/Mouse/Rat GRK2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human GRK2 in direct ELISAs and Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2089B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human GRK2
Pro468-Leu689
Accession # P25098
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human, Mouse, and Rat GRK2 by Western Blot. View Larger

Detection of Human, Mouse, and Rat GRK2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line, Ramos human Burkitt's lymphoma cell line, LL/2 mouse Lewis lung carcinoma cell line, H4‑II‑E‑C3 rat hepatoma cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat GRK2 Monoclonal Antibody (Catalog # MAB43391) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for GRK2 at approximately 76 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection  of Human GRK2 by Simple WesternTM. View Larger

Detection of Human GRK2 by Simple WesternTM. Simple Western lane view shows lysates of Ramos human Burkitt's lymphoma cell line and THP‑1 human acute monocytic leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for GRK2 at approximately 80 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse/
Rat GRK2 Monoclonal Antibody (Catalog # MAB43391). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GRK2

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK2 is a member of the beta ARK subfamily, and was originally termed beta ‑adrenergic receptor kinase 1 ( beta ARK1).

Long Name
G protein-coupled Receptor Kinase 2
Entrez Gene IDs
156 (Human); 110355 (Mouse); 25238 (Rat)
Alternate Names
ADRBK1; adrenergic, beta, receptor kinase 1; BARK; BARK1; BARK1beta-ARK-1; Beta-ARK1; Beta-ARK-1; EC 2.7.11; EC 2.7.11.15; FLJ16718; G-protein coupled receptor kinase 2; GRK2; GRK2beta-adrenergic receptor kinase 1

Product Datasheets

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