Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ILKAP/PP2C delta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ILKAP
Met1-His392
Accession # Q9H0C8

Specificity

Detects endogenous human ILKAP in Western blots. In direct ELISAs, this antibody shows weak cross-reactivity with PP2C alpha (4%), PP2C beta (6%), and PP2C gamma (1%).

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat ILKAP/PP2C delta Antibody

Detection of Human/Mouse/Rat ILKAP/PP2Cd antibody by Western Blot.

Detection of Human/Mouse/Rat ILKAP/PP2C delta by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat ILKAP/PP2Cd Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4554) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ILKAP/PP2C delta at approximately 47 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Applications for Human/Mouse/Rat ILKAP/PP2C delta Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ILKAP/PP2C delta

Integrin Linked Kinase 1 Associated Phosphatase (ILKAP), also called PP2Cdelta, is a serine/threonine phosphatase that is stimulated by Mn2+ ions but inhibited by Mg2+ and is insensitive to the phosphatase inhibitor okadaic acid. It binds to ILK1 and dephosphorylates it, decreasing the stimulation of the GSK-3 beta pathway by cell adhesion without affecting Akt signaling. Transfection studies have shown that ILKAP activity inhibits cell cycle progression from the G1 to S phase. ILKAP is expressed in many tissues but is highest in skeletal and cardiac muscle. Its gene is found in a region of chromosome 2 that is frequently deleted in oral cancers.

Long Name

Integrin-Linked Kinase-Associated Serine/Threonine Phosphatase 2C

Alternate Names

PP2C delta

Entrez Gene IDs

80895 (Human); 67444 (Mouse); 64538 (Rat)

Gene Symbol

ILKAP

UniProt

Q9H0C8

Additional ILKAP/PP2C delta Products

Product Documents for Human/Mouse/Rat ILKAP/PP2C delta Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat ILKAP/PP2C delta Antibody

For research use only

Related Research Areas

Epigenetics
Phosphatases and Regulators

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