Human/Mouse/Rat Jagged 1 APC-conjugated Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat Jagged 1. In direct ELISAs, no cross‑reactivity with recombinant human Jagged 2 is observed. Stains human Jagged 1 transfectants but not the parental cell line or human Jagged 2 transfectants in flow cytometry. 
  • Source
    Monoclonal Mouse IgG2B Clone # 188331
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0-derived recombinant human Jagged 1
    Ser32-Asp296
    Accession # P78504
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Jagged 1 in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human/Mouse/Rat Jagged 1 APC‑conjugated Monoclonal Antibody (Catalog # FAB1277A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Jagged 1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Mouse Anti-Human/Mouse/Rat Jagged 1 APC‑conjugated Monoclonal Antibody (Catalog # FAB1277A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Jagged 1

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185‑229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 shares 97% and 96% aa identity with mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1‑8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).

  • References:
    1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
    2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
    3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
    4. Lai, E.C. (2004) Development 131:965.
    5. Milner, L.A. et al. (1994) Blood 83:2057.  
    6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504
    7. Linheng, L. et al. (1998) Immunity 8:43.
    8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
    9. Oda, T. et al. (1997) Nat. Genet. 16:235.
    10. Oda, T. et al. (1997) Genomics 43:376.
  • Entrez Gene IDs:
    182 (Human); 29146 (Rat)
  • Alternate Names:
    AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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