Human/Mouse/Rat LAR Alexa Fluor® 532-conjugated Antibody

Catalog #: AF3004X Datasheet / COA / SDS

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AF3004X-100UG

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Human/Mouse/Rat LAR Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat LAR in Western blots. In Western blots, this antibody detects both full-length protein and the cleaved LAR extracellular (E) domain. The antibody does not cross react with PTPR sigma.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LAR
Ala27-Glu1251
Accession # NP_569707

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: LAR

Leucocyte Antigen-Related (LAR) tyrosine phosphatase, also known as Protein Tyrosine Phoshatase, Receptor-type F (PTPRF), is an integral membrane protein with a non-glycosylated molecular weight of 207 kDa. The extracellular domain is cleaved near the cell membrane by a subtilisin-like endoprotease to a molecular weight of 139 kDa. Depending on cellular conditions, the extracellular domain may remain associated with the rest of the molecule or can be shed into the extracellular medium. This antibody was made against the extracellular domain of LAR and detects both full-length and cleaved LAR.

Long Name

Leukocyte Antigen-Related Tyrosine Phosphatase

Entrez Gene IDs

5792 (Human); 104121 (Mouse)

Alternate Names

LAR; LCA-homolog; protein tyrosine phosphatase, receptor type, F; PTPRF; receptor type, F polypeptide; receptor-linked protein-tyrosine phosphatase LAR; receptor-type tyrosine-protein phosphatase F

Product Datasheets

Product Datasheet

COA

Related Research Areas

FAQs

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