Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Chinese Hamster

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all LAR Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LAR
Ala27-Glu1251
Accession # NP_569707

Specificity

Detects human, mouse, and rat LAR in Western blots. In Western blots, this antibody detects both full-length protein and the cleaved LAR extracellular (E) domain. The antibody does not cross react with PTPR sigma.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat LAR Antibody

Detection of Human/Mouse/Rat LAR antibody by Western Blot.

Detection of Human/Mouse/Rat LAR by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Neuro-2A mouse neuroblastoma cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat LAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3004) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Bands were detected for LAR at approximately 207 kDa for full-length LAR and 139 kDa for cleaved LAR (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat LAR Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Neuro-2A mouse neuroblastoma cell line, and Y3-Ag rat myeloid cell line

Reviewed Applications

Read 1 review rated 5 using AF3004 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LAR

Leucocyte Antigen-Related (LAR) tyrosine phosphatase, also known as Protein Tyrosine Phoshatase, Receptor-type F (PTPRF), is an integral membrane protein with a non-glycosylated molecular weight of 207 kDa. The extracellular domain is cleaved near the cell membrane by a subtilisin-like endoprotease to a molecular weight of 139 kDa. Depending on cellular conditions, the extracellular domain may remain associated with the rest of the molecule or can be shed into the extracellular medium. This antibody was made against the extracellular domain of LAR and detects both full-length and cleaved LAR.

Long Name

Leukocyte Antigen-Related Tyrosine Phosphatase

Alternate Names

PTPRF

Entrez Gene IDs

5792 (Human); 104121 (Mouse)

Gene Symbol

PTPRF

UniProt

NP_569707

Additional LAR Products

Product Documents for Human/Mouse/Rat LAR Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat LAR Antibody

For research use only

Related Research Areas

Epigenetics
Phosphatases and Regulators

Citations for Human/Mouse/Rat LAR Antibody

Customer Reviews for Human/Mouse/Rat LAR Antibody (1)

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1 Customer Rating
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  • Human/Mouse/Rat LAR Antibody
    Name: Sijia He
    Application: Western Blot
    Sample Tested: mouse primary hepatocyte
    Species: Mouse
    Verified Customer | Posted 11/01/2018
    Tried this antibody, works very well,the bands were specific and clean, located between 115-250 kDa area. Highly recommend.
    Human/Mouse/Rat LAR Antibody AF3004

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