|Detection of Human, Mouse, and Rat NEDP1/SENP8 by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line, beta TC‑6 mouse beta cell insulinoma cell line, and NRK‑49F rat fibroblasts. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat NEDP1/SENP8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7760) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NEDP1/SENP8 at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
NEDP1 (NEDD8-specific protease 1; also DEN1/Deneddylase-1 Protease, cysteine 2 and SENP8/Sentrin-specific protease 8) is a 24-26 kDa cytoplasmic cysteine protease that belongs to the Ulp/peptidase C48 family of enzymes. It is widely expressed, and is involved in NEDD8-associated ubiquitination. NEDD8 is a 9 kDa polypeptide that covalently binds to, and activates cullin-1, a molecule associated with a ubiquitin ligase complex. NEDD8-activated cullin promotes the transfer of ubiquitin to a limited number of target substrates involved in the cell cycle. This has the effect of controlling the levels of cell cycle regulators within the cell. NEDP1 influences these activities in two ways; first, it converts preNEDD8 into its mature form, and second, it deconjugates NEDD8 from its bound substrates. Human NEDP1 is 212 amino acids (aa) in length. It possesses an acetylated initiating methionine plus a protease domain that spans aa 11-174. Over an equivalent sequence, full-length human NEDP1 shares 92% aa sequence identity with mouse NEDP1. The mouse NEDP1 molecule contains a nine aa extension at the C-terminus not shared by the human molecule.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided