Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Neurolysin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Neurolysin
aa 38-704

Specificity

Detects human, mouse, and rat Neurolysin Neurolysin in Western blots and human Neurolysin in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Meprin alpha, rhMeprin beta subunit, and rhTHOP1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Neurolysin Antibody

Detection of Human, Mouse, and Rat Neurolysin antibody by Western Blot.

Detection of Human, Mouse, and Rat Neurolysin by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, bEnd.3 mouse endothelioma cell line, rat cortical neuron tissue, and human brain (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Neurolysin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3814) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurolysin at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat Neurolysin Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Neurolysin (Catalog # 3814-ZN), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line, bEnd.3 mouse endothelioma cell line, rat cortical neuron tissue, and human brain (cortex) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Neurolysin

Neurolysin, also known as Oligopeptidase M (mitochondrial peptidase), soluble angiotensin II-binding protein and endopeptidase EC 3.4.24.16 (1, 2), is a homologue of Thimet Ologopeptidase (THOP1), a zinc peptidase of the M3 family that also includes mitochondrial intermediate peptidase. Neurolysin expresses in two forms with the difference of an N-terminal transit peptide that targets the localization to mitochondria (1). The shorter form exists in cytosol. The recombinant human Neurolysin is expressed without the transit peptide and is isolated from the cytosol. Like THOP1, Neurolysin is capable of cleaving a number of vasoactive peptides such as bradykinin and neurotensin (3). All known substrates of THOP1 and Neurolysin contain 17 or fewer amino acids.

References

  1. Serizawa, A. et al. (1995) J. Biol. Chem. 270:2092.
  2. Barett, A.J. and J.M. Chen (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 356, Elsevier Academic Press, San Diego.
  3. Norman, M.U. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1978.

Alternate Names

AGTBP, Neurotensin Endopeptidase, NLN, Oligopeptidase M

Entrez Gene IDs

57486 (Human); 75805 (Mouse); 117041 (Rat)

Gene Symbol

NLN

Additional Neurolysin Products

Product Documents for Human/Mouse/Rat Neurolysin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat Neurolysin Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Customer Reviews for Human/Mouse/Rat Neurolysin Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat Neurolysin Antibody and earn rewards!

Have you used Human/Mouse/Rat Neurolysin Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies