NIPP1 (Nuclear Inhibitory polypeptide of Protein Phosphatase 1; also ARD-1 and PPP1R8) is a 41 kDa intracellular protein that belongs to no known molecular family. Although it is ubiquitously expressed, it does undergo alternative splicing, and distinct isoforms are associated with distinct tissues. In the nucleus, NIPP1 complexes with PP1c, targeting it to either RNA or chromatin. This is accompanied by the additional recruitment of other molecules such as Sap155 and PRC2. When nonphosphorylated, NIPP1 blocks PP1c activity; when phosphorylated, NIPP1 allows for PP1c activity, and this impacts the processes of both pre-mRNA splicing and gene silencing. Human NIPP1 is 351 amino acids (aa) in length. It contains an N-terminal FHA domain (aa 49-101), an NLS (aa 185-209), a PP1 binding site (aa 191-210), another NLS (aa 210-240) and an RNA binding site (aa 330-351). There are two potential splice variants. Both possess alternative start sites, one at Met143 (called NIPP1 beta ), and another at Met225 (called ARD-1 or NIPP1 gamma ). Full-length human NIPP1 shares 98% aa sequence identity with mouse NIPP1.
Human/Mouse/Rat NIPP1 Alexa Fluor® 405‑conjugated Antibody
R&D Systems | Catalog # AF7240V
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Ala2-Ile351
Accession # Q12972
Specificity
Clonality
Host
Isotype
Applications for Human/Mouse/Rat NIPP1 Alexa Fluor® 405‑conjugated Antibody
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
Background: NIPP1
Long Name
Alternate Names
Gene Symbol
UniProt
Additional NIPP1 Products
Product Documents for Human/Mouse/Rat NIPP1 Alexa Fluor® 405‑conjugated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse/Rat NIPP1 Alexa Fluor® 405‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
Related Research Areas
Customer Reviews
There are currently no reviews for this product. Be the first to review Human/Mouse/Rat NIPP1 Alexa Fluor® 405‑conjugated Antibody and earn rewards!
Have you used Human/Mouse/Rat NIPP1 Alexa Fluor® 405‑conjugated Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars