phospho-NMDAR2B (Y1336) Antibody

R&D Systems | Catalog # PPS057

R&D Systems
Discontinued Product
PPS057 has been discontinued. View all NMDAR2B products.

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all NMDAR2B Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Y1336 of NMDA R, NR2B Subunit

Specificity

Human, mouse, and rat ~180 kDa NMDA Receptor NR2B subunit phosphorylated at Y1336 in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for phospho-NMDAR2B (Y1336) Antibody

Detects Phospho-GRIN2B/NMDAR2B (Y1336) by Western Blot.

Detects Phospho-GRIN2B/NMDAR2B (Y1336) by Western Blot.

Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 180 kDa NR2B subunit of the NMDAR phosphorylated at Y1336 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l PPase). The blot is identical to the control except that it was incubated in l PPase (1200 units for 30 minutes) before being exposed to the Anti-Phospho-NMDA NR2B subunit (Y1336). The immunolabeling is completely eliminated by treatment with l PPase.

Applications for phospho-NMDAR2B (Y1336) Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 mL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 mg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ ‑20° C is recommended. Product is stable at ≤ ‑20° C for at least 1 year.

Background: NMDAR2B

NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics and mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes that code for NR2 subunits (NR2A-D). The NR2B subunit generates a high conductance NMDAR. Human NR2B (also NR3;GRIN2B and GluRe2) is a 180-200 kDa, 1458 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 531 aa N-terminal extracellular domain (ECD), and a 646 aa cytoplasmic region. At least three tyrosines are known to be phosphorylated by Fyn in the cytoplasmic region. These include Y1252, Y1336 and Y1472. While much is known about Y1472, little information is available about the consequences of Y1252 and Y1336 phosphorylation.

References

  1. Stephenson, F.A. (2001) Curr. Drug Targets 2:233.
  2. Cull-candy, S.G. and D.N. Leszkiewicz (2004) Sci. STKE re16 (2004).
  3. Prybylowski, K. and R.J. Wenthold (2004) J. Biol. Chem. 279:9673.
  4. Ishii, T. et al. (1993) J. Biol. Chem. 268:2836.
  5. Hess, S.D. et al. (1996) J. Pharmacol. Exp. Ther. 278:808.
  6. Nakazawa, T. et al. (2001) J. Biol. Chem. 276:693.

Long Name

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2B

Alternate Names

glutamate [NMDA] receptor subunit epsilon-2, glutamate receptor subunit epsilon-2, glutamate receptor, ionotropic, N-methyl D-aspartate 2B, hNR3, MGC142180, NMDA R, NR2B Subunit, NMDAR2B, NMDAR2BMGC142178, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3

Entrez Gene IDs

2904 (Human)

Gene Symbol

GRIN2B

Additional NMDAR2B Products

Product Documents for phospho-NMDAR2B (Y1336) Antibody

Certificate of Analysis

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Product Specific Notices for phospho-NMDAR2B (Y1336) Antibody

For research use only

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