Human/Mouse/Rat Phospho-GRIN2B/NMDAR2B (Y1336) Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Human, mouse, and rat ~180 kDa NMDA Receptor NR2B subunit phosphorylated at Y1336 in Western blots.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide corresponding to amino acid residues surrounding the phospho-Y1336 of NMDA R, NR2B Subunit
  • Formulation
    100 mL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 mg/mL BSA and 50% glycerol.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detects Phospho-GRIN2B/NMDAR2B (Y1336) by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 180 kDa NR2B subunit of the NMDAR phosphorylated at Y1336 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l PPase). The blot is identical to the control except that it was incubated in l PPase (1200 units for 30 minutes) before being exposed to the Anti-Phospho-NMDA NR2B subunit (Y1336). The immunolabeling is completely eliminated by treatment with l PPase.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    For long-term storage, ≤ ‑20° C is recommended. Product is stable at ≤ ‑20° C for at least 1 year.
Background: GRIN2B/NMDAR2B
NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics and mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes that code for NR2 subunits (NR2A-D). The NR2B subunit generates a high conductance NMDAR. Human NR2B (also NR3;GRIN2B and GluRe2) is a 180-200 kDa, 1458 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 531 aa N-terminal extracellular domain (ECD), and a 646 aa cytoplasmic region. At least three tyrosines are known to be phosphorylated by Fyn in the cytoplasmic region. These include Y1252, Y1336 and Y1472. While much is known about Y1472, little information is available about the consequences of Y1252 and Y1336 phosphorylation.
  • References:
    1. Stephenson, F.A. (2001) Curr. Drug Targets 2:233.
    2. Cull-candy, S.G. and D.N. Leszkiewicz (2004) Sci. STKE re16 (2004).
    3. Prybylowski, K. and R.J. Wenthold (2004) J. Biol. Chem. 279:9673.
    4. Ishii, T. et al. (1993) J. Biol. Chem. 268:2836.
    5. Hess, S.D. et al. (1996) J. Pharmacol. Exp. Ther. 278:808.
    6. Nakazawa, T. et al. (2001) J. Biol. Chem. 276:693.
  • Long Name:
    Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2B
  • Entrez Gene IDs:
    2904 (Human)
  • Alternate Names:
    GRIN2B; NMDA R, NR2B Subunit; NMDAR2B; NR2B
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