Human/Mouse/Rat Phospho-GRIN2B/NMDAR2B (Y1472) Antibody

R&D Systems | Catalog # PPS014

R&D Systems
Discontinued Product
PPS014 has been discontinued. View all GRIN2B/NMDAR2B products.

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GRIN2B/NMDAR2B Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Y1472 of NMDA R, NR2B subunit

Specificity

Human, mouse, rat ~180 kDa NMDAR NR2B-subunit protein phosphorylated at Y1472

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Phospho-GRIN2B/NMDAR2B (Y1472) Antibody

Detection of Phospho-GRIN2B/NMDAR2B (Y1472) antibody by Western Blot

Detection of Phospho-GRIN2B/NMDAR2B (Y1472) by Western Blot

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR (Control). The phosphospecificty of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-NMDA NR2B subunit (Y1472). The immunolabeling is completely eliminated by treatment with lambda -PPase.

Applications for Human/Mouse/Rat Phospho-GRIN2B/NMDAR2B (Y1472) Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Rat brain (hippocampus) tissue

Reviewed Applications

Read 1 review rated 3 using PPS014 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 150 mM NaCl, 10 mM HEPES, 100 μg/mL BSA and 50% glycerol, pH 7.5.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GRIN2B/NMDAR2B

Rat NMDA receptor subunit 2B (NR2B) is a 1456 amino acid, 180 kDa, 3-transmembrane member of the glutamate-gated ion channel family. NMDA are heteromultimers of at least two NR1 subunits and two NR2 or two (presumably) NR3 subunits. Upon ligand binding, NMDA receptors open and allow cation transit. The NR1 subunits bind glycine and are essential for the physical formation of the ion channel. NR2 subunits apparently bind glutamate and impart pharmacological properties to the receptor complex. Fully functional receptors require the presence of both glycine and glutamate. The NR1-NR2B complex has the highest affinity for glutamate. Both NR1 and NR2B subunits can be phosphorylated by PKC. On NR1, PKC phosphorylates on serine at 890; on NR2B, PKC phosphorylates on tyrosine at 1472. Phosphorylation is considered to promote ion transit and impact NR2B interaction with downstream signaling molecules.

References

  1. Grosshans, D.R. and M.D. Browning (2001) J. Neurochem. 76:737.
  2. Cheung, H. et al. (2003) J. Neurochem. 86:1441.
  3. Gibb, A.J. (2004) Trends Neurosci. 27:7.
  4. Liu, Y. and J. Zhang (2000) Chin. Med. J. 113:948.

Long Name

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2B

Alternate Names

NMDA R, NR2B Subunit, NMDAR2B, NR2B

Entrez Gene IDs

2904 (Human)

Gene Symbol

GRIN2B

Additional GRIN2B/NMDAR2B Products

Product Documents for Human/Mouse/Rat Phospho-GRIN2B/NMDAR2B (Y1472) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Phospho-GRIN2B/NMDAR2B (Y1472) Antibody

For research use only

Citations for Human/Mouse/Rat Phospho-GRIN2B/NMDAR2B (Y1472) Antibody

Customer Reviews for Human/Mouse/Rat Phospho-GRIN2B/NMDAR2B (Y1472) Antibody (1)

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  • Human/Mouse/Rat Phospho-GRIN2B/NMDAR2B (Y1472) Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: mouse brain lysate
    Species: Mouse
    Verified Customer | Posted 08/16/2017
    Simple Western lane view shows two prominent signals at ~100 kDa and ~181 kDa (expected size: ~180 kDa) in lysates prepared from mouse brains. Results vary (no/additional signals) depending on sample type, protein conc. and lysis buffer.
    This run was performed under reducing conditions using the 12-230 kDa separation system in combination with the rabbit detection system (Lysis buffer from ARY014 proteome profiler kit, protein concentration: ~0.5µg/µl).
    Human/Mouse/Rat Phospho-GRIN2B/NMDAR2B (Y1472) Antibody PPS014

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