Human/Mouse/Rat Phospho-GRIN2B/NMDAR2B (Y1472) Antibody

Catalog # Availability Size / Price Qty
PPS014
Detection of Phospho-GRIN2B/NMDAR2B (Y1472) by Western Blot
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Citations (1)
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Human/Mouse/Rat Phospho-GRIN2B/NMDAR2B (Y1472) Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Human, mouse, rat ~180 kDa NMDAR NR2B-subunit protein phosphorylated at Y1472
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Y1472 of NMDA R, NR2B subunit
Formulation
100 μL in 150 mM NaCl, 10 mM HEPES, 100 μg/mL BSA and 50% glycerol, pH 7.5.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Phospho-GRIN2B/NMDAR2B (Y1472) antibody by Western Blot View Larger

Detection of Phospho-GRIN2B/NMDAR2B (Y1472) by Western Blot Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR (Control). The phosphospecificty of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-NMDA NR2B subunit (Y1472). The immunolabeling is completely eliminated by treatment with lambda -PPase.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GRIN2B/NMDAR2B

Rat NMDA receptor subunit 2B (NR2B) is a 1456 amino acid, 180 kDa, 3-transmembrane member of the glutamate-gated ion channel family. NMDA are heteromultimers of at least two NR1 subunits and two NR2 or two (presumably) NR3 subunits. Upon ligand binding, NMDA receptors open and allow cation transit. The NR1 subunits bind glycine and are essential for the physical formation of the ion channel. NR2 subunits apparently bind glutamate and impart pharmacological properties to the receptor complex. Fully functional receptors require the presence of both glycine and glutamate. The NR1-NR2B complex has the highest affinity for glutamate. Both NR1 and NR2B subunits can be phosphorylated by PKC. On NR1, PKC phosphorylates on serine at 890; on NR2B, PKC phosphorylates on tyrosine at 1472. Phosphorylation is considered to promote ion transit and impact NR2B interaction with downstream signaling molecules.

References
  1. Grosshans, D.R. and M.D. Browning (2001) J. Neurochem. 76:737.
  2. Cheung, H. et al. (2003) J. Neurochem. 86:1441.
  3. Gibb, A.J. (2004) Trends Neurosci. 27:7.
  4. Liu, Y. and J. Zhang (2000) Chin. Med. J. 113:948.
Long Name
Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2B
Entrez Gene IDs
2904 (Human)
Alternate Names
GRIN2B; NMDA R, NR2B Subunit; NMDAR2B; NR2B

Product Datasheets

Citation for Human/Mouse/Rat Phospho-GRIN2B/NMDAR2B (Y1472) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Interleukin-1-induced interleukin-6 synthesis is mediated by the neutral sphingomyelinase/Src kinase pathway in neurones.
    Authors: Tsakiri N, Kimber I, Rothwell NJ, Pinteaux E
    Br. J. Pharmacol., 2007;153(4):775-83.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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