Human/Mouse/Rat Plexin A2 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5486A
Detection of Plexin A2 in bEnd.3 Mouse Cell Line by Flow Cytometry.
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Human/Mouse/Rat Plexin A2 APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects mouse and rat Plexin A2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Plexin A1, rmPlexin A3, and recombinant human Plexin B1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Plexin A2
Met35-Pro1237
Accession # P70207
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Plexin A2 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Plexin A2 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Human/Mouse/Rat Plexin A2 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5486A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Plexin A2 antibody in PC-12 Rat Cell Line antibody by Flow Cytometry. View Larger

Detection of Plexin A2 in PC‑12 Rat Cell Line by Flow Cytometry. PC-12 rat adrenal pheochromocytoma cell line was stained with Goat Anti-Human/Mouse/Rat Plexin A2 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5486A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Plexin A2 antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of Plexin A2 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human/Mouse/Rat Plexin A2 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5486A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Plexin A2

Plexin A2 (also OCT) is a 230-250 kDa member of the plexin-A subfamily, plexin family of proteins. It is found on cerebellar granule cells, sensory neurons, CD31+ LYVE+ lymphoid endothelial cells, osteoblasts, and cardiac neural crest cells. It blocks neurite outgrowth and lymphatic capillary formation, but promotes cell migration and osteogenesis, and does so by serving as a receptor for Sema6A and B, and as a coreceptor with neuropilins for Sema3A and C. Notably, it would appear to contribute to the receptor complex for BMPs by interacting with both BMPR1A/ALK3 and BMPR1B/ALK6 in conjunction with BMPR2. Mature mouse Plexin A2 is an 1860 amino acid (aa) type I transmembrane homodimeric glycoprotein. It contains a 1203 aa extracellular domain (ECD) and a 636 aa cytoplasmic region. The ECD contains one Sema domain (aa 35-508), three PSI domains (aa 510-855) and four IPT regions (aa 858-1228). Over aa 35-1237, mouse Plexin A2 shares 96% aa sequence identity with human Plexin A2.

Entrez Gene IDs
5362 (Human); 18845 (Mouse); 289392 (Rat)
Alternate Names
FLJ11751; FLJ30634; KIAA0463plexin 2; OCT; OCTSemaphorin receptor OCT; Plexin A2; PLXN2; PLXN2plexin-A2; PLXNA2; transmembrane protein OCT

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