Human/Mouse/Rat PSD‑95 Antibody

R&D Systems | Catalog # PPS059

R&D Systems
Discontinued Product
PPS059 has been discontinued. View all PSD-95 products.

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PSD-95 Primary Antibodies »

Product Specifications

Immunogen

PSD-95

Specificity

Human, mouse, and rat ~95 kDa PSD-95 in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat PSD‑95 Antibody

Detection of PSD-95 antibody by Western Blot.

Detection of PSD‑95 by Western Blot.

Western blot of rat hippocampus lysate showing specific immunolabeling of the approximately 95 kDa PSD-95 protein.

Applications for Human/Mouse/Rat PSD‑95 Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 mL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 mg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ ‑20° C is recommended. Product is stable at ≤ ‑20° C for at least 1 year.

Background: PSD-95

PSD-95 (postsynaptic density-95 kDa; also SAP90) is a 95 kDa member of the MAGUK (membrane-associated guanylate kinase) family of molecules. It is a multidomain protein that serves as a scaffold, or anchor, for the organization of structural and signal transduction pathways in neurons. It is capable of forming multimers, generating large complexes that impact the size and strength of synapses. The molecule is highly conserved across species, being 99% amino acid identical, human to rodent. In rats, PSD-95 is 724 amino acids (aa) in length, and contains three consecutive 80 aa PDZ domains (aa 65 - 393), followed by an SH3 domain (aa 432-490) and a C-terminal guanylate kinase domain (aa 534-709). The PZD (PSD/Discs-large/ZO-1) domains in PSD-95 are globular structures that bind NMDA receptor subunits and neuroligin (a transmembrane intercellular neuron adhesion molecule). The SH3 domain binds proline-rich motifs on enzymes, and may interact with either kainate receptor subunits or SHANK, another scaffold protein. The guanylate kinase binds microtubules-associated protein and kainate receptor subunits. PSD-95 is regulated by palmitoylation on C3 and C5. When present, palmitate promotes AMPA receptor clustering and neurotransmission. Phosphorylation also regulates PSD-95 activity. Both S290 and S295 are known to be phosphorylated. These modifications are suggested to retain PSD-95 in the synapse and/or regulate PZD interactions with target proteins.

References

  1. Sierralta, J. and C. Mendoza (2004) Brain Res. Rev. 47:105.
  2. Kim, E. and M. Sheng (2004) Nat. Rev. Neurosci. 5:771.
  3. Hata, Y. and Y. Takai (1999) Cell. Mol. Life Sci. 56:461.
  4. Fukata, M. et al. (2004) Neuron 44:987.
  5. Sabio, G. et al. (2004) Biochem. J. 380:19.
  6. Jaffe, H. et al. (2004) Biochem. Biophys. Res. Commun. 321:210.

Long Name

Postsynaptic Density Protein 95/Disks Large Homolog 4

Alternate Names

DLG4, PSD95, SAP90

Entrez Gene IDs

1742 (Human)

Gene Symbol

DLG4

Additional PSD-95 Products

Product Documents for Human/Mouse/Rat PSD‑95 Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat PSD‑95 Antibody

For research use only

Citations for Human/Mouse/Rat PSD‑95 Antibody

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