Human/Mouse RNA Polymerase II/POLR2A Alexa Fluor® 532-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: RNA Polymerase II/POLR2A

POLR2A (DNA-directed RNA Polymerase II subunit RPB1; also subunit A) is a 200‑220 kDa member of the RNA polymerase beta -chain family of proteins. It is ubiquitously expressed and represents the largest subunit of RNA polymerase II, the catalytic mechanism for the synthesis of mRNA. Human POLR2A is 1970 amino acids (aa) in length. It contains an N-terminus with six RNA polymerase domains (aa 15‑1079), and a C-terminus (CTD) with 52 seven aa repeats (‑YSPTSPS‑) that serve as a platform for polymerase subunit interaction. When these sequences are hyperphosphorylated, this subunit is called RNA pol IIo; when hypophosphorylated, the subunit becomes RNA pol IIa. Isoforms exist that show a 60 aa substitution for aa 1‑95, a 24 aa substitution for aa 1‑445, and an Arg substitution for aa 1498‑1536. Full-length human and mouse POLR2A sequences differ by only one amino acid (Thr1856Ala).

Product Datasheets

Related Research Areas

• Epigenetics