Detection of Human and Mouse RNA Polymerase II/POLR2A by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, MCF‑7 human breast cancer cell line, and HT‑2 mouse T cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse RNA Polymerase II/POLR2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6160) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for RNA Polymerase II/POLR2A at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RNA Polymerase II/POLR2A
POLR2A (DNA-directed RNA Polymerase II subunit RPB1; also subunit A) is a 200‑220 kDa member of the RNA polymerase beta -chain family of proteins. It is ubiquitously expressed and represents the largest subunit of RNA polymerase II, the catalytic mechanism for the synthesis of mRNA. Human POLR2A is 1970 amino acids (aa) in length. It contains an N-terminus with six RNA polymerase domains (aa 15‑1079), and a C-terminus (CTD) with 52 seven aa repeats (‑YSPTSPS‑) that serve as a platform for polymerase subunit interaction. When these sequences are hyperphosphorylated, this subunit is called RNA pol IIo; when hypophosphorylated, the subunit becomes RNA pol IIa. Isoforms exist that show a 60 aa substitution for aa 1‑95, a 24 aa substitution for aa 1‑445, and an Arg substitution for aa 1498‑1536. Full-length human and mouse POLR2A sequences differ by only one amino acid (Thr1856Ala).
DNA-directed RNA Polymerase II Subunit RPB1
Entrez Gene IDs:
5430 (Human); 20020 (Mouse); 363633 (Rat)
DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 126.96.36.199; EC 188.8.131.52; hRPB220; hsRPB1; MGC75453; POLR2; POLR2A; POLRA; polymerase (RNA) II (DNA directed) polypeptide A (220kD); polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; RNA Pol 2; RNA Polymerase 2; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RNAPII; RPB1; RPBh1; RpIILS; RPO2; RPOL2
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