Human/Mouse ROR gamma t/RORC2/NR1F3 Alexa Fluor® 488-conjugated Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects recombinant human ROR gamma t/RORC2/NR1F3 by direct ELISAs and detects human and mouse ROR gamma t/RORC2/NR1F3 in flow cytometry.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1181A
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    E.coli-derived recombinant human ROR gamma t/RORC2/NR1F3
    Met1-Gln100
    Accession # P51449
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of ROR gamma t/RORC2/NR1F3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Rabbit Anti-Human/Mouse ROR gamma t/RORC2/NR1F3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC9125G) and Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # IC1051G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
Detection of ROR gamma t/RORC2/NR1F3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Rabbit Anti-Human/Mouse ROR gamma t/RORC2/NR1F3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC9125G) and Rat Anti-Mouse CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB554A). Quadrant markers were set based on control antibody staining (Catalog # IC1051G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ROR gamma t/RORC2/NR1F3
Retinoic acid-related Orphan Receptor gamma (ROR gamma, TOR, RORC; NR1F3) is a member of the orphan nuclear receptor family. ROR gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. ROR gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5' A/T-rich extensions. An N-terminal isoform of ROR gamma, ROR gamma t, has been shown to be specifically expressed in the thymus.
  • Long Name:
    Retinoic Acid-Related Orphan Receptor gamma t
  • Entrez Gene IDs:
    6097 (Human)
  • Alternate Names:
    ROR gamma t; RORC2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Alexa Fluor® 488-conjugated Control

Ctrl IC105G
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Rabbit IgG Alexa Fluor® 488-conjugated Antibody

Ctrl IC1051G
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