Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # GK1.5
View all CD4 Primary Antibodies »

Product Specifications

Immunogen

Mouse CTL clone V4

Specificity

Reacts with mouse CD4, an antigen co-receptor on the T cell surface which interacts with MHC II molecules on antigen presenting cells.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse CD4 APC‑conjugated Antibody

Detection of CD4 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CD4 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) or (B) Rat IgG2BAllophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CD4 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes

Reviewed Applications

Read 2 reviews rated 4.5 using FAB554A in the following applications:

Spectra Viewer

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD4

CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

Alternate Names

CD4

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Gene Symbol

CD4

Additional CD4 Products

Product Documents for Mouse CD4 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD4 APC‑conjugated Antibody

For research use only

Citations for Mouse CD4 APC‑conjugated Antibody

Customer Reviews for Mouse CD4 APC‑conjugated Antibody (2)

4.5 out of 5
2 Customer Ratings
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  • Mouse CD4 Biotinylated Antibody
    Name: Debangshu Samanta
    Application: Flow Cytometry
    Sample Tested: Breast cancer tissue
    Species: Mouse
    Verified Customer | Posted 05/03/2019
  • Mouse CD4 Biotinylated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Murine Splenocytes (C57B6)
    Species: Mouse
    Verified Customer | Posted 01/30/2019
    Mouse splenocytes stained with anti-CD4 (RL1).
    Mouse CD4 APC‑conjugated Antibody FAB554A

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