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- Species ReactivityMouse
- SpecificityReacts with mouse CD4, an antigen co-receptor on the T cell surface which interacts with MHC II molecules on antigen presenting cells.
- SourceMonoclonal Rat IgG2B Clone # GK1.5
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenMouse CTL clone V4
- FormulationSupplied in a saline solution containing BSA and Sodium Azide.
- LabelAllophycocyanin
Applications
-
Recommended
ConcentrationSample
-
Flow Cytometry10 µL/106 cellsSee below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
|
Flow Cytometry
|
Detection of CD4 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE‑conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB554A) or (B) Rat IgG2B Allophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins. |
Preparation and Storage
- ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageProtect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD4
CD4
is a type I membrane glycoprotein belonging to the immunoglobulin
superfamily. It is expressed predominantly on thymocytes and a subset of
mature T lymphocytes. CD4 functions in collaboration with the T cell
receptor in the recognition of peptide antigens that are presented by
class II major histocompatibility complexes. CD4 also has been shown to
be a coreceptor of HIV entry and specifically binds gp120, the external
envelope glycoprotein of HIV.
- Entrez Gene IDs:920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 493775 (Feline)
- Alternate Names:CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4
Related Research Areas
- Conventional/Classical Dendritic Cells
- gamma delta T Cells
- Infectious Diseases
- Leukocyte Receptors/Markers in Allergy and Asthma
- Leukocyte Receptors/Markers in Tfh Cell Differentiation and Function
- LTi Cells
- Lymphoid Lineage Markers
- Natural Killer T (NKT) Cells
- Regulatory T Cells (Tregs)
- Th1 Cells
- Th17 Cells
- Th2 Cells
- Th22 Cells
- Th9 Cells
Isotype Controls
Description
Application
Cat#
Citations
Images
Rat IgG2B APC‑conjugated Isotype Control
Ctrl IC013A 4View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
|---|---|---|---|---|
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Staining Reagents
Description
Application
Cat#
Citations
Images
Flow Cytometry Staining Buffer (1X)
Flow FC001 6View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
|---|---|---|---|---|
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Flow Cytometry Mouse Lyse Buffer (10X)
Flow FC003 4View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
|---|---|---|---|---|
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Flow Cytometry Human Lyse Buffer (10X)
Flow FC002 1View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
|---|---|---|---|---|
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Calculators
Reconstitution Calculator
Reconstitution Calculator
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
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