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Mouse CD4 APC-conjugated Antibody

Catalog #: FAB554A
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB554A-100
FAB554A-025
Detection of CD4 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (2)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD4 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Reacts with mouse CD4, an antigen co-receptor on the T cell surface which interacts with MHC II molecules on antigen presenting cells.
Source
Monoclonal Rat IgG2B Clone # GK1.5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse CTL clone V4
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD4 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD4 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) or (B) Rat IgG2BAllophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD4

CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

Entrez Gene IDs
920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)
Alternate Names
CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Citations for Mouse CD4 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Mitigation of Tacrolimus-Associated Nephrotoxicity by PLGA Nanoparticulate Delivery Following Multiple Dosing to Mice while Maintaining its Immunosuppressive Activity
    Authors: A Alshamsan, Z Binkhathla, MA Kalam, W Qamar, H Kfouri, M Alghonaim, A Lavasanifa
    Sci Rep, 2020;10(1):6675.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Ouabain Attenuates Sepsis-Induced Immunosuppression in Mice by Activation and Anti-Apoptosis of T Cells
    Authors: R Niu, H Gao, Y Zhou, J Zhang
    Med. Sci. Monit., 2018;24(0):2720-2727.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Isotype Controls

Rat IgG2B APC‑conjugated Isotype Control

IC013A
5 Citations
1 Image
Ctrl

Staining Reagents

Flow Cytometry Human Lyse Buffer (10X)

FC002
2 Citations
1 Review
Flow

Flow Cytometry Mouse Lyse Buffer (10X)

FC003
5 Citations
1 Review
Flow

Flow Cytometry Staining Buffer (1X)

FC001
26 Citations
5 Reviews
Flow

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