Human/Mouse S1P5/EDG-8 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3964A
Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Human S1P5/EDG-8 and eGFP by Flow Cytometry.
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Product Details
Citations (1)
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Human/Mouse S1P5/EDG-8 APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse S1P5/EDG‑8. Stains human and mouse S1P5/EDG‑8 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 282503
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human S1P5/EDG‑8
Met1-Asp398
Accession # Q9H228
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of S1P<SUB>5</SUB>/EDG-8 antibody in HEK293 Human Cell Line Transfected with Human S1P<SUB>5</SUB>/EDG-8 and eGFP antibody by Flow Cytometry. View Larger

Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Human S1P5/EDG-8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human S1P5/EDG-8 and eGFP was stained with and either (A) Mouse Anti-Human S1P5/EDG‑8 APC‑conjugated Monoclonal Antibody (Catalog # FAB3964A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of S1P<sub>5</sub>/EDG-8 antibody in HEK293 Human Cell Line Transfected with Mouse S1P<sub>5</sub>/EDG-8 and eGFP antibody by Flow Cytometry. View Larger

Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Mouse S1P5/EDG-8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P5/EDG-8 and eGFP was stained with and either (A) Mouse Anti-Human S1P5/EDG-8 APC-conjugated Monoclonal Antibody (Catalog # FAB3964A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P5/EDG-8

S1P5 is also known as EDG-8 and nerve growth factor-related G-protein-coupled receptor-1 (NRG-1). S1P5 is a 398 amino acid (aa) seven-transmembrane receptor putative glycoprotein that binds the lysolipid phosphoric acid mediator, sphingosine 1-phosphate. Extracellular portions of human S1P5 show 96% and 97% aa identity with mouse and rat S1P5, respectively. Isoform 1 is expressed at a low level in peripheral tissues. Isoform 2 has an alternate C-terminal that is 88 aa shorter and is expressed mainly in brain, spleen, and PBMC. S1P5 is upregulated in large granular lymphocytic leukemias.

Long Name
Endothelial Differentiation Gene 8
Entrez Gene IDs
53637 (Human); 94226 (Mouse); 60399 (Rat)
Alternate Names
EDG8; Edg-8; EDG8S1P receptor Edg-8; Endothelial differentiation G-protein-coupled receptor 8; endothelial differentiation, sphingolipid G-protein-coupled receptor, 8; LPB4; S1P receptor 5; S1P5; S1PR5; sphingosine 1-phosphate receptor 5; sphingosine 1-phosphate receptor EDG8; Sphingosine 1-phosphate receptor Edg-8; sphingosine-1-phosphate receptor 5; SPPR-1; SPPR-2

Product Datasheets

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Citation for Human/Mouse S1P5/EDG-8 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Cytomegalovirus Infection Impairs the Mobilization of Tissue-Resident Innate Lymphoid Cells into the Peripheral Blood Compartment in Response to Acute Exercise
    Authors: E Cho, B Theall, J Stampley, J Granger, NM Johannsen, BA Irving, G Spielmann
    Viruses, 2021;13(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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