Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 282503
View all S1P5/EDG-8 Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human S1P5/EDG‑8
Met1-Asp398
Accession # Q9H228

Specificity

Detects human and mouse S1P

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse S1P5/EDG-8 APC-conjugated Antibody

Detection of S1P5/EDG-8 antibody in HEK293 Human Cell Line Transfected with Human S1P5/EDG-8 and eGFP antibody by Flow Cytometry.

Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Human S1P5/EDG-8 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human S1P5/EDG-8 and eGFP was stained with and either (A) Mouse Anti-Human S1P5/EDG‑8 APC‑conjugated Monoclonal Antibody (Catalog # FAB3964A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse S1P5/EDG-8 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human S1P5/EDG-8 and eGFP and HEK293 human embryonic kidney cell line transfected with mouse S1P5/EDG-8 and eGFP

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P5/EDG-8

S1P5 is also known as EDG-8 and nerve growth factor-related G-protein-coupled receptor-1 (NRG-1). S1P5 is a 398 amino acid (aa) seven-transmembrane receptor putative glycoprotein that binds the lysolipid phosphoric acid mediator, sphingosine 1-phosphate. Extracellular portions of human S1P5 show 96% and 97% aa identity with mouse and rat S1P5, respectively. Isoform 1 is expressed at a low level in peripheral tissues. Isoform 2 has an alternate C-terminal that is 88 aa shorter and is expressed mainly in brain, spleen, and PBMC. S1P5 is upregulated in large granular lymphocytic leukemias.

Long Name

Endothelial Differentiation Gene 8

Alternate Names

EDG8, LPB4, S1PR5, SPPR-1, SPPR-2

Entrez Gene IDs

53637 (Human); 94226 (Mouse); 60399 (Rat)

Gene Symbol

S1PR5

UniProt

Q9H228

Additional S1P5/EDG-8 Products

Product Documents for Human/Mouse S1P5/EDG-8 APC-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse S1P5/EDG-8 APC-conjugated Antibody

For research use only

Citations for Human/Mouse S1P5/EDG-8 APC-conjugated Antibody

Customer Reviews for Human/Mouse S1P5/EDG-8 APC-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse S1P5/EDG-8 APC-conjugated Antibody and earn rewards!

Have you used Human/Mouse S1P5/EDG-8 APC-conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies