Human/Mouse S1P5/EDG-8 APC-conjugated Antibody Summary
Accession # Q9H228
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Human S1P5/EDG-8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human S1P5/EDG-8 and eGFP was stained with and either (A) Mouse Anti-Human S1P5/EDG‑8 APC‑conjugated Monoclonal Antibody (Catalog # FAB3964A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Mouse S1P5/EDG-8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P5/EDG-8 and eGFP was stained with and either (A) Mouse Anti-Human S1P5/EDG-8 APC-conjugated Monoclonal Antibody (Catalog # FAB3964A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
S1P5 is also known as EDG-8 and nerve growth factor-related G-protein-coupled receptor-1 (NRG-1). S1P5 is a 398 amino acid (aa) seven-transmembrane receptor putative glycoprotein that binds the lysolipid phosphoric acid mediator, sphingosine 1-phosphate. Extracellular portions of human S1P5 show 96% and 97% aa identity with mouse and rat S1P5, respectively. Isoform 1 is expressed at a low level in peripheral tissues. Isoform 2 has an alternate C-terminal that is 88 aa shorter and is expressed mainly in brain, spleen, and PBMC. S1P5 is upregulated in large granular lymphocytic leukemias.
Citation for Human/Mouse S1P5/EDG-8 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Cytomegalovirus Infection Impairs the Mobilization of Tissue-Resident Innate Lymphoid Cells into the Peripheral Blood Compartment in Response to Acute Exercise
Authors: E Cho, B Theall, J Stampley, J Granger, NM Johannsen, BA Irving, G Spielmann
Sample Types: Whole Cells
Applications: Flow Cytometry
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