Secreted Frizzled-related protein (sFRP-3), also known as FRZB, Fritz, Frzb1 and FRP-3, is expressed in the developing bone and cartilage as well as several adult tissues. It functions as an antagonist of Wnt signaling.
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA Detection (Matched Antibody Pair)
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 Clone # 133912
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human sFRP-3
Ala33-Asn325
Accession # AAB51298
Ala33-Asn325
Accession # AAB51298
Specificity
Detects human sFRP-3 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant mouse sFRP-3 is observed and no cross-reactivity with recombinant human sFRP-1, -2, or -4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for sFRP-3 Antibody (133912) [Biotin]
Human sFRP-3 ELISA Standard Curve
Recombinant Human sFRP‑3 (Catalog # 192-SF) was serially diluted and captured by Mouse Anti-Human/Mouse sFRP‑3 Monoclonal Antibody (Catalog # MAB1921) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Mouse sFRP‑3 Monoclonal Antibody (Catalog # MAB192) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for sFRP-3 Antibody (133912) [Biotin]
Human/Mouse sFRP-3 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: sFRP-3
Long Name
Secreted Frizzled Related Protein 3
Alternate Names
Fritz, FRZB, Frzb1, sFRP3
Gene Symbol
FRZB
UniProt
Additional sFRP-3 Products
Product Documents for sFRP-3 Antibody (133912) [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for sFRP-3 Antibody (133912) [Biotin]
For research use only
Related Research Areas
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