Secreted Frizzled-related protein (sFRP-3), also known as FRZB, Fritz, Frzb1 and FRP-3, is expressed in the developing bone and cartilage as well as several adult tissues. It functions as an antagonist of Wnt signaling.
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Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA Detection (Matched Antibody Pair)
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 Clone # 133912
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human sFRP-3
Ala33-Asn325
Accession # AAB51298
Ala33-Asn325
Accession # AAB51298
Specificity
Detects human sFRP-3 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant mouse sFRP-3 is observed and no cross-reactivity with recombinant human sFRP-1, -2, or -4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for sFRP-3 Antibody (133912) [Biotin]
Human sFRP-3 ELISA Standard Curve
Recombinant Human sFRP‑3 (Catalog # 192-SF) was serially diluted and captured by Mouse Anti-Human/Mouse sFRP‑3 Monoclonal Antibody (Catalog # MAB1921) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Mouse sFRP‑3 Monoclonal Antibody (Catalog # MAB192) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for sFRP-3 Antibody (133912) [Biotin]
Human/Mouse sFRP-3 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: sFRP-3
Long Name
Secreted Frizzled Related Protein 3
Alternate Names
Fritz, FRZB, Frzb1, sFRP3
Gene Symbol
FRZB
UniProt
Additional sFRP-3 Products
Product Documents for sFRP-3 Antibody (133912) [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for sFRP-3 Antibody (133912) [Biotin]
For research use only
Related Research Areas
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