Human/Mouse Shisa-4 Alexa Fluor® 350-conjugated Antibody

Catalog #: AF6365U Datasheet / COA / SDS

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AF6365U-100UG

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All Shisa-4 Antibodies

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Human/Mouse Shisa-4 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Shisa‑4 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Shisaâ€‘4
Glu29-Thr85
Accession # Q96DD7

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Shisa-4

Shisa-4 (Shisa is a form of Japanese sculpture that involves faces/heads; also known as C1orf40, PRMP and TMEM58) is a 26‑32 kDa member of the Shisa-4 subfamily, Shisa family of proteins. It may be found associated with the ER of embryonic limb bud cells, and based on other Shisa proteins, perhaps antagonizes either Wnt or FGF signaling. Shisa-4 is also described as a plasma membrane protein on oligodendroglia that links the ECM to the actin cytoskeleton. Mature human Shisa-4 is a 170 amino acid (aa) type I transmembrane protein. It contains a 60 aa extracellular domain (aa 28‑87) plus an 89 aa cytoplasmic region that possesses a Pro-rich segment (aa 131‑195). Over aa 29‑85, human Shisa-4 shares 96% aa identity with mouse Shisa-4.

Entrez Gene IDs

149345 (Human); 77552 (Mouse); 360848 (Rat)

Alternate Names

C1orf40; chromosome 1 open reading frame 40; FLJ18233; hShisa4; MGC129812; MGC129813; protein shisa-4; shisa homolog 4 (Xenopus laevis); Shisa4; Shisa-4; TMEM58; Transmembrane protein 58MGC102893