Shisa-4 Antibody

R&D Systems | Catalog # AF6365

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Shisa-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Shisa‑4
Glu29-Thr85
Accession # Q96DD7

Specificity

Detects human and mouse Shisa‑4 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Shisa-4 Antibody

Detection of Human Shisa-4 antibody by Western Blot.

Detection of Human Shisa‑4 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Shisa-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6365) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Shisa-4 at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Shisa-4 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Shisa-4

Shisa-4 (Shisa is a form of Japanese sculpture that involves faces/heads; also known as C1orf40, PRMP and TMEM58) is a 26‑32 kDa member of the Shisa-4 subfamily, Shisa family of proteins. It may be found associated with the ER of embryonic limb bud cells, and based on other Shisa proteins, perhaps antagonizes either Wnt or FGF signaling. Shisa-4 is also described as a plasma membrane protein on oligodendroglia that links the ECM to the actin cytoskeleton. Mature human Shisa-4 is a 170 amino acid (aa) type I transmembrane protein. It contains a 60 aa extracellular domain (aa 28‑87) plus an 89 aa cytoplasmic region that possesses a Pro-rich segment (aa 131‑195). Over aa 29‑85, human Shisa-4 shares 96% aa identity with mouse Shisa-4.

Alternate Names

Shisa4, TMEM58

Entrez Gene IDs

149345 (Human); 77552 (Mouse); 360848 (Rat)

Gene Symbol

SHISA4

UniProt

Q96DD7 (Human)

Additional Shisa-4 Products

Product Documents for Shisa-4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Shisa-4 Antibody

For research use only

Related Research Areas

FGF Family
Wnt Signaling Modulators

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