Detection of Human Shisa‑4 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Shisa‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6365) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Shisa‑4 at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Shisa-4 (Shisa is a form of Japanese sculpture that involves faces/heads; also known as C1orf40, PRMP and TMEM58) is a 26‑32 kDa member of the Shisa-4 subfamily, Shisa family of proteins. It may be found associated with the ER of embryonic limb bud cells, and based on other Shisa proteins, perhaps antagonizes either Wnt or FGF signaling. Shisa-4 is also described as a plasma membrane protein on oligodendroglia that links the ECM to the actin cytoskeleton. Mature human Shisa-4 is a 170 amino acid (aa) type I transmembrane protein. It contains a 60 aa extracellular domain (aa 28‑87) plus an 89 aa cytoplasmic region that possesses a Pro-rich segment (aa 131‑195). Over aa 29‑85, human Shisa-4 shares 96% aa identity with mouse Shisa-4.
Entrez Gene IDs:
149345 (Human); 77552 (Mouse); 360848 (Rat)
C1orf40; chromosome 1 open reading frame 40; FLJ18233; hShisa4; MGC129812; MGC129813; protein shisa-4; shisa homolog 4 (Xenopus laevis); Shisa4; Shisa-4; TMEM58; Transmembrane protein 58MGC102893
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