Shisa-4 (Shisa is a form of Japanese sculpture that involves faces/heads; also known as C1orf40, PRMP and TMEM58) is a 26‑32 kDa member of the Shisa-4 subfamily, Shisa family of proteins. It may be found associated with the ER of embryonic limb bud cells, and based on other Shisa proteins, perhaps antagonizes either Wnt or FGF signaling. Shisa-4 is also described as a plasma membrane protein on oligodendroglia that links the ECM to the actin cytoskeleton. Mature human Shisa-4 is a 170 amino acid (aa) type I transmembrane protein. It contains a 60 aa extracellular domain (aa 28‑87) plus an 89 aa cytoplasmic region that possesses a Pro-rich segment (aa 131‑195). Over aa 29‑85, human Shisa-4 shares 96% aa identity with mouse Shisa-4.
Human/Mouse Shisa‑4 Alexa Fluor™ Plus 488‑conjugated Antibody
R&D Systems | Catalog # AF6365AFP488
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Specificity
Detects human and mouse Shisa‑4 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human/Mouse Shisa‑4 Alexa Fluor™ Plus 488‑conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Shisa-4
Alternate Names
Shisa4, TMEM58
Gene Symbol
SHISA4
UniProt
Additional Shisa-4 Products
Product Documents for Human/Mouse Shisa‑4 Alexa Fluor™ Plus 488‑conjugated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse Shisa‑4 Alexa Fluor™ Plus 488‑conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
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- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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