Human/Mouse SPRY2 Alexa Fluor® 750-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: SPRY2

SPRY2 (sprouty homolog 2) is a 35 kDa member of the sprouty family of proteins. It is widely expressed in embryonic and adult tissues, including endothelium. SPRY2 is considered a negative regulator of Ras/ERK signaling. For example, it appears to form a trimeric complex with PKCδ and PKD1, blocking ERK phosphorylation. It also interacts with a number of other molecules, including CBL and SIAH2, which likely limit its availability. Human SPRY2 is 315 amino acids (aa) in length and contains one CBL-TKB binding site (aa 53‑59) that is phosphorylated at Tyr55, a Ser-rich region (aa 125‑131), a Cys-rich domain (aa 178‑301) and a PxxPxR motif that mediates ERK inhibitory activity. Over aa 1‑175, human SPRY2 shares 94% aa identity with mouse SPRY2.

Product Datasheets

Related Research Areas

• FGF Family
• Phospholipases, Small GTPases, and Other Molecules in the Akt Pathway