SPRY2 (sprouty homolog 2) is a 35 kDa member of the sprouty family of proteins. It is widely expressed in embryonic and adult tissues, including endothelium. SPRY2 is considered a negative regulator of Ras/ERK signaling. For example, it appears to form a trimeric complex with PKC delta and PKD1, blocking ERK phosphorylation. It also interacts with a number of other molecules, including CBL and SIAH2, which likely limit its availability. Human SPRY2 is 315 amino acids (aa) in length and contains one CBL-TKB binding site (aa 53‑59) that is phosphorylated at Tyr55, a Ser-rich region (aa 125‑131), a Cys-rich domain (aa 178‑301) and a PxxPxR motif that mediates ERK inhibitory activity. Over aa 1‑175, human SPRY2 shares 94% aa identity with mouse SPRY2.
Human/Mouse SPRY2 Antibody
R&D Systems | Catalog # AF6157
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Met1-Ala175
Accession # O43597
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse SPRY2 Antibody
Detection of Human and Mouse SPRY2 by Western Blot.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, PC-3 human prostate cancer cell line, and C2C12 mouse myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse SPRY2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6157) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SPRY2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Mouse SPRY2 by Simple WesternTM.
Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and SH-SY5Y human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SPRY2 at approximately 45 kDa (as indicated) using 50 µg/mL of Goat Anti-Human/Mouse SPRY2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6157) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Applications for Human/Mouse SPRY2 Antibody
Simple Western
Sample: C2C12 mouse myoblast cell line and SH‑SY5Y human neuroblastoma cell line
Western Blot
Sample: SH‑SY5Y human neuroblastoma cell line, PC‑3 human prostate cancer cell line, and C2C12 mouse myoblast cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: SPRY2
Long Name
Alternate Names
Gene Symbol
UniProt
Additional SPRY2 Products
Product Documents for Human/Mouse SPRY2 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse SPRY2 Antibody
For research use only
Related Research Areas
Citations for Human/Mouse SPRY2 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars