Human/Mouse SSEA-1 Alexa Fluor® 594-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
SSEA‑1 in D3 Mouse Cell Line. SSEA‑1 was detected in immersion fixed D3 mouse embryonic stem cell line using Mouse Anti-Human/Mouse SSEA‑1 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # IC2155T; red) at 10 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).
- Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
- Fox, N. et al. (1983) Cancer Res. 43:669.
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