Human/Mouse SSEA-4 APC-conjugated Antibody

(6 citations)
(1 Review)
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
  • Source
    Monoclonal Mouse IgG3 Clone # MC-813-70
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    2120Ep human embryonal carcinoma cell line
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of SSEA‑4 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA‑4 APC‑conjugated Monoclonal Antibody (Catalog # FAB1435A, filled histogram) or isotype control antibody (Catalog # IC007A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SSEA-4

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

  • References:
    1. Shevinsky, L.H. et al. (1982) Cell 30:697.
    2. Kannagi, R. et al. (1983) EMBO J. 2:2355.
    3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
    4. Draper, J.S. et al. (2002) J. Anat. 200:249.
  • Long Name:
    Stage-specific Embryonic Antigen-4
  • Alternate Names:
    SSEA4; SSEA-4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Prospectively Isolated Human Bone Marrow Cell-Derived MSCs Support Primitive Human CD34-Negative Hematopoietic Stem Cells.
    Authors: Matsuoka Y, Nakatsuka R, Sumide K, Kawamura H, Takahashi M, Fujioka T, Uemura Y, Asano H, Sasaki Y, Inoue M, Ogawa H, Takahashi T, Hino M, Sonoda Y
    Stem Cells, 2015;33(5):1554-65.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. JMJD5 regulates cell cycle and pluripotency in human embryonic stem cells.
    Authors: Zhu H, Hu S, Baker J
    Stem Cells, 2014;32(8):2098-110.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Human embryonic stem cell-derived mesenchymal stroma cells (hES-MSCs) engraft in vivo and support hematopoiesis without suppressing immune function: implications for off-the shelf ES-MSC therapies.
    Authors: Li O, Tormin A, Sundberg B, Hyllner J, Le Blanc K, Scheding S
    PLoS ONE, 2013;8(1):e55319.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. High concentration of synthetic serum, stepwise equilibration and slow cooling as an efficient technique for large-scale cryopreservation of human embryonic stem cells.
    Authors: Lee JY, Lee JE, Kim DK, Yoon TK, Chung HM, Lee DR
    Fertil. Steril., 2010;93(3):976-85.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Evaluation of ABCG2 expression in human embryonic stem cells: crossing the same river twice?
    Authors: Sarkadi B, Orban TI, Szakacs G, Varady G, Schamberger A, Erdei Z, Szebenyi K, Homolya L, Apati A
    Stem Cells, 2010;28(1):174-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Expression and functional analysis of G1 to S regulatory components reveals an important role for CDK2 in cell cycle regulation in human embryonic stem cells.
    Authors: Neganova I, Zhang X, Atkinson S, Lako M
    Oncogene, 2009;28(1):20-30.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG3 APC-conjugated Antibody

Ctrl IC007A 1  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Flow Cytometry Human/Mouse SSEA‑4 APC‑conjugated Antibody FAB1435A
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 Flow Human Anonymous 10/26/2015
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Flow Cytometry Human/Mouse SSEA‑4 APC‑conjugated Antibody FAB1435A
Flow Cytometry: Human/Mouse SSEA‑4 APC‑conjugated Antibody [FAB1435A]

Summary

ApplicationFlow Cytometry
Sample TestedHuman embryonic stem cells (H9 from Wicell)
SpeciesHuman

Other Experimental Details

Other Experimental DetailshPSC were grown for 20 passages as monolayers on matrigel.
Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: PBS+.1% FBS
Dilution: 1:20

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