Human/Mouse SSEA-4 Antibody Summary
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data

Detection of SSEA‑4 in NTera‑2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # MAB1435, filled histogram) or isotype control antibody (Catalog # MAB007, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

SSEA‑4 in BG01V Human Stem Cells. SSEA-4 was detected in immersion fixed BG01V human embryonic stem cells cultured on irradiated mouse embryonic fibroblasts (Catalog # PSC001) using 10 µg/mL Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # MAB1435) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SSEA-4
SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).
Product Datasheets
Citations for Human/Mouse SSEA-4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient
Authors: J Lotila, T Hyvärinen, H Skottman, L Airas, S Narkilahti, S Hagman
Oncogene, 2022;63(0):102865.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Generation of iPSC line FAMRCi010-A from patient with restrictive cardiomyopathy carrying genetic variant FLNC p.Gly2011Arg
Authors: K Perepelina, A Khudiakov, N Rodina, A Boytsov, T Vavilova, A Zlotina, P Sokolnikov, A Kostareva
Stem Cell Research, 2021;59(0):102639.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Comparative evaluation of isogenic mesodermal and ectomesodermal chondrocytes from human iPSCs for cartilage regeneration
Authors: MS Lee, MJ Stebbins, H Jiao, HC Huang, EM Leiferman, BE Walczak, SP Palecek, EV Shusta, WJ Li
Science Advances, 2021;7(21):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Generation of iPSC line (FAMRCi009-A) from patient with familial progressive cardiac conduction disorder carrying genetic variant FLNC p.Val2264Met
Authors: N Rodina, A Khudiakov, K Perepelina, A Muravyev, A Boytsov, A Zlotina, P Sokolnikov, A Kostareva
Stem Cell Research, 2021;59(0):102640.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The combination of dibenzazepine and a DOT1L inhibitor enables a stable maintenance of human na�ve-state pluripotency in non-hypoxic conditions
Authors: W Isono, T Kawasaki, JK Ichida, T Ayabe, O Hiraike, A Umezawa, H Akutsu
Regenerative Therapy, 2020;15(0):161-168.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of two induced pluripotent stem cell lines (FAMRCi005-A and FAMRCi005-B) from patient carrying genetic variant LMNA p.Asp357Val
Authors: P Klauzen, K Perepelina, A Khudiakov, A Zlotina, Y Fomicheva, T Pervunina, T Vershinina, A Kostareva, A Malashiche
Stem Cell Res, 2020;43(0):101719.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of two iPSC lines (FAMRCi004-A and FAMRCi004-B) from patient with familial progressive cardiac conduction disorder carrying genetic variant DSP p.His1684Arg
Authors: A Khudiakov, K Perepelina, P Klauzen, A Zlotina, K Gusev, E Kaznacheye, A Malashiche, A Kostareva
Stem Cell Res, 2020;43(0):101720.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Grafted human induced pluripotent stem cells improve the outcome of spinal cord injury: modulation of the lesion microenvironment
Authors: T Bellák, Z Fekécs, D Török, Z Táncos, C Nemes, Z Tézsla, L Gál, S Polgári, J Kobolák, A Dinnyés, A Nógrádi, K Pajer
Scientific Reports, 2020;10(1):22414.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of two iPSC lines (FAMRCi006-A and FAMRCi006-B) from patient with dilated cardiomyopathy and Emery-Dreifuss muscular dystrophy associated with genetic variant LMNAp.Arg527Pro
Authors: K Perepelina, P Klauzen, A Khudiakov, A Zlotina, Y Fomicheva, D Rudenko, M Gordeev, A Sergushich, A Malashiche, A Kostareva
Stem Cell Res, 2020;43(0):101714.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of two iPSC lines (FAMRCi007-A and FAMRCi007-B) from patient with Emery-Dreifuss muscular dystrophy and heart rhythm abnormalities carrying genetic variant LMNA p.Arg249Gln
Authors: K Perepelina, A Kostina, P Klauzen, A Khudiakov, M Rabino, S Crasto, A Zlotina, Y Fomicheva, A Sergushich, M Oganesian, A Dmitriev, A Kostareva, E Di Pasqual, A Malashiche
Stem Cell Res, 2020;47(0):101895.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Activation of PDGF pathway links LMNA mutation to dilated cardiomyopathy
Authors: J Lee, V Termglinch, S Diecke, I Itzhaki, CK Lam, P Garg, E Lau, M Greenhaw, T Seeger, H Wu, JZ Zhang, X Chen, IP Gil, M Ameen, K Sallam, JW Rhee, JM Churko, R Chaudhary, T Chour, PJ Wang, MP Snyder, HY Chang, I Karakikes, JC Wu
Nature, 2019;0(0):.
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Ciliary gene RPGRIP1L is required for hypothalamic arcuate neuron development
Authors: L Wang, AJ De Solis, Y Goffer, KE Birkenbach, SE Engle, R Tanis, JM Levenson, X Li, R Rausch, M Purohit, JY Lee, J Tan, MC De Rosa, CA Doege, HL Aaron, GJ Martins, JC Brüning, D Egli, R Costa, N Berbari, RL Leibel, G Stratigopo
JCI Insight, 2019;4(3):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Effects of the Extracts from Fruit and Stem of Camellia japonica on Induced Pluripotency and Wound Healing
Authors: H Jeon, JY Kim, JK Choi, E Han, CL Song, J Lee, YS Cho
J Clin Med, 2018;7(11):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Small non-coding RNA landscape of extracellular vesicles from human stem cells
Authors: S Kaur, AG Abu-Shahba, RO Paananen, H Hongisto, H Hiidenmaa, H Skottman, R Seppänen-K, B Mannerströ
Sci Rep, 2018;8(1):15503.
Species: Human
Sample Types: Whole Cells
Applications: Differentiation -
DMSO increases efficiency of genome editing at two non-coding loci
Authors: G Stratigopo, MC De Rosa, CA LeDuc, RL Leibel, CA Doege
PLoS ONE, 2018;13(6):e0198637.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
Authors: PA De Sousa, R Steeg, E Wachter, K Bruce, J King, M Hoeve, S Khadun, G McConnachi, J Holder, A Kurtz, S Seltmann, J Dewender, S Reimann, G Stacey, O O'Shea, C Chapman, L Healy, H Zimmermann, B Bolton, T Rawat, I Atkin, A Veiga, B Kuebler, BM Serano, T Saric, J Hescheler, O Brüstle, M Peitz, C Thiele, N Geijsen, B Holst, C Clausen, M Lako, L Armstrong, SK Gupta, AJ Kvist, R Hicks, A Jonebring, G Brolén, A Ebneth, A Cabrera-So, P Foerch, M Geraerts, TC Stummann, S Harmon, C George, I Streeter, L Clarke, H Parkinson, PW Harrison, A Faulconbri, L Cherubin, T Burdett, C Trigueros, MJ Patel, C Lucas, B Hardy, R Predan, J Dokler, M Brajnik, O Keminer, O Pless, P Gribbon, C Claussen, A Ringwald, B Kreisel, A Courtney, TE Allsopp
Stem Cell Res, 2017;20(0):105-114.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A Comprehensive TALEN-Based Knockout Library for Generating Human Induced Pluripotent Stem Cell-Based Models for Cardiovascular Diseases
Authors: I Karakikes, V Termglinch, DA Cepeda, J Lee, S Diecke, A Hendel, I Itzhaki, M Ameen, R Shrestha, H Wu, N Ma, NY Shao, T Seeger, NA Woo, KD Wilson, E Matsa, MH Porteus, V Sebastiano, JC Wu
Circ. Res, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
The effects of glomerular and tubular renal progenitors and derived extracellular vesicles on recovery from acute kidney injury
Authors: A Ranghino, S Bruno, B Bussolati, A Moggio, V Dimuccio, M Tapparo, L Biancone, P Gontero, B Frea, G Camussi
Stem Cell Res Ther, 2017;8(1):24.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Generation of iPSC line from desmin-related cardiomyopathy patient carrying splice site mutation of DES gene
Authors: A Khudiakov, D Kostina, A Zlotina, T Nikulina, A Sergushich, A Gudkova, A Tomilin, A Malashiche, A Kostareva
Stem Cell Res, 2017;24(0):77-80.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Effect of microgravity on the mesenchymal stem cell characteristics of limbal fibroblasts
Authors: SI Pao, KH Chien, HT Lin, MC Tai, JT Chen, CM Liang
J Chin Med Assoc, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
High expression FUT1 and B3GALT5 is an independent predictor of postoperative recurrence and survival in hepatocellular carcinoma
Authors: HH Kuo, RJ Lin, JT Hung, CB Hsieh, TH Hung, FY Lo, MY Ho, CT Yeh, YL Huang, J Yu, AL Yu
Sci Rep, 2017;7(1):10750.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Alloimmune Responses of Humanized Mice to Human Pluripotent Stem Cell Therapeutics
Authors: NG Kooreman, PE de Almeida, JP Stack, RV Nelakanti, S Diecke, NY Shao, RJ Swijnenbur, V Sanchez-Fr, E Matsa, C Liu, AJ Connolly, JF Hamming, PHA Quax, MA Brehm, DL Greiner, LD Shultz, JC Wu
Cell Rep, 2017;20(8):1978-1990.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Modeling Glanzmann thrombasthenia using patient specific iPSCs and restoring platelet aggregation function by CD41 overexpression
Authors: L Hu, L Du, Y Zhao, W Li, Q Ouyang, D Zhou, G Lu, G Lin
Stem Cell Res, 2017;20(0):14-20.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity
Authors: O Guillevic, S Ferratge, J Pascaud, C Driancourt, J Boyer-Di-P, G Uzan
PLoS ONE, 2016;11(4):e0152993.
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr -
Characterization and molecular profiling of PSEN1 familial Alzheimer's disease iPSC-derived neural progenitors.
Authors: Sproul A, Jacob S, Pre D, Kim S, Nestor M, Navarro-Sobrino M, Santa-Maria I, Zimmer M, Aubry S, Steele J, Kahler D, Dranovsky A, Arancio O, Crary J, Gandy S, Noggle S
PLoS ONE, 2014;9(1):e84547.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Telomerase-mediated telomere elongation from human blastocysts to embryonic stem cells.
Authors: Zeng S, Liu L, Sun Y, Xie P, Hu L, Yuan D, Chen D, Ouyang Q, Lin G, Lu G
J Cell Sci, 2014;127(0):752-62.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells.
Authors: Luo, Lan, Kawakatsu, Miho, Guo, Chao-Wan, Urata, Yoshishi, Huang, Wen-Jing, Ali, Haytham, Doi, Hanako, Kitajima, Yuriko, Tanaka, Takayuki, Goto, Shinji, Ono, Yusuke, Xin, Hong-Bo, Hamano, Kimikazu, Li, Tao-Shen
Sci Rep, 2014;4(0):3779.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Human dermis harbors distinct mesenchymal stromal cell subsets.
Authors: Vaculik C, Schuster C, Bauer W, Iram N, Pfisterer K, Kramer G, Reinisch A, Strunk D, Elbe-Burger A
J. Invest. Dermatol., 2012;132(3):563-74.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
Authors: Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM
Stem Cells Dev., 2012;21(3):404-10.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells.
Authors: Kim SY, Kim MJ, Jung H
Stem Cells Dev., 2012;21(8):1272-86.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
BMC Vet Res, 2012;8(0):150.
Species: Canine
Sample Types: Whole Cells
Applications: Flow Cytometry -
Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium.
Authors: Hayashi, Ryuhei, Ishikawa, Yuki, Ito, Miyuki, Kageyama, Tomofumi, Takashiba, Kuniko, Fujioka, Tsuyoshi, Tsujikawa, Motokazu, Miyoshi, Hiroyuki, Yamato, Masayuki, Nakamura, Yukio, Nishida, Kohji
PLoS ONE, 2012;7(9):e45435.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of HIV-1 resistant and functional macrophages from hematopoietic stem cell-derived induced pluripotent stem cells.
Authors: Kambal A, Mitchell G, Cary W, Gruenloh W, Jung Y, Kalomoiris S, Nacey C, McGee J, Lindsey M, Fury B, Bauer G, Nolta JA, Anderson JS
Mol. Ther., 2011;19(3):584-93.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
A bioinformatic assay for pluripotency in human cells.
Authors: Muller FJ, Schuldt BM, Williams R, Mason D, Altun G, Papapetrou EP, Danner S, Goldmann JE, Herbst A, Schmidt NO, Aldenhoff JB, Laurent LC, Loring JF
Nat. Methods, 2011;8(4):315-7.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH
FEBS Lett., 2009;583(3):561-7.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Tumor initiating potential of side population cells in human gastric cancer.
Authors: Fukuda K, Saikawa Y, Ohashi M, Kumagai K, Kitajima M, Okano H, Matsuzaki Y, Kitagawa Y
Int. J. Oncol., 2009;34(5):1201-7.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells.
Authors: Fong H, Hohenstein KA, Donovan PJ
Stem Cells, 2008;26(8):1931-8.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.
Authors: Reed SA, Johnson SE
J. Cell. Physiol., 2008;215(2):329-36.
Species: Equine
Sample Types: Whole Cells
Applications: ICC -
Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells.
Authors: Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ
Stem Cells, 2008;26(6):1436-43.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.
Authors: Josephson R, Ording CJ, Liu Y, Shin S, Lakshmipathy U, Toumadje A, Love B, Chesnut JD, Andrews PW, Rao MS, Auerbach JM
Stem Cells, 2007;25(2):437-46.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
Authors: Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
Stem Cells, 2007;25(2):490-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells.
Authors: Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH
FEBS Lett., 2006;580(25):5869-74.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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Reviews for Human/Mouse SSEA-4 Antibody
Average Rating: 4.2 (Based on 9 Reviews)
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1:200 dilution worked well.
I used to check for SSEA-4 in my iPSCs and it worked great