Human/Mouse SSEA-4 Antibody

Catalog # Availability Size / Price Qty
MAB1435
MAB1435-SP
Detection of SSEA‑4 in NTera‑2 Human Cell Line by Flow Cytometry.
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Product Details
Citations (43)
FAQs
Supplemental Products
Reviews (9)

Human/Mouse SSEA-4 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
Source
Monoclonal Mouse IgG3 Clone # MC-813-70
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
2120Ep human embryonal carcinoma cell line
Formulation
Lyophilized from a 0.2 μm filtered solution in MES with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of SSEA-4 antibody in NTera-2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of SSEA‑4 in NTera‑2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # MAB1435, filled histogram) or isotype control antibody (Catalog # MAB007, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Immunocytochemistry SSEA‑4 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC). View Larger

SSEA‑4 in BG01V Human Stem Cells. SSEA-4 was detected in immersion fixed BG01V human embryonic stem cells cultured on irradiated mouse embryonic fibroblasts (Catalog # PSC001) using 10 µg/mL Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # MAB1435) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SSEA-4

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

References
  1. Shevinsky, L.H. et al. (1982) Cell 30:697.
  2. Kannagi, R. et al. (1983) EMBO J. 2:2355.
  3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
  4. Draper, J.S. et al. (2002) J. Anat. 200:249.
Long Name
Stage-specific Embryonic Antigen-4
Alternate Names
SSEA4; SSEA-4

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Citations for Human/Mouse SSEA-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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  1. Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient
    Authors: J Lotila, T Hyvärinen, H Skottman, L Airas, S Narkilahti, S Hagman
    Oncogene, 2022;63(0):102865.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Generation of iPSC line FAMRCi010-A from patient with restrictive cardiomyopathy carrying genetic variant FLNC p.Gly2011Arg
    Authors: K Perepelina, A Khudiakov, N Rodina, A Boytsov, T Vavilova, A Zlotina, P Sokolnikov, A Kostareva
    Stem Cell Research, 2021;59(0):102639.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Comparative evaluation of isogenic mesodermal and ectomesodermal chondrocytes from human iPSCs for cartilage regeneration
    Authors: MS Lee, MJ Stebbins, H Jiao, HC Huang, EM Leiferman, BE Walczak, SP Palecek, EV Shusta, WJ Li
    Science Advances, 2021;7(21):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Generation of iPSC line (FAMRCi009-A) from patient with familial progressive cardiac conduction disorder carrying genetic variant FLNC p.Val2264Met
    Authors: N Rodina, A Khudiakov, K Perepelina, A Muravyev, A Boytsov, A Zlotina, P Sokolnikov, A Kostareva
    Stem Cell Research, 2021;59(0):102640.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. The combination of dibenzazepine and a DOT1L inhibitor enables a stable maintenance of human na�ve-state pluripotency in non-hypoxic conditions
    Authors: W Isono, T Kawasaki, JK Ichida, T Ayabe, O Hiraike, A Umezawa, H Akutsu
    Regenerative Therapy, 2020;15(0):161-168.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Generation of two induced pluripotent stem cell lines (FAMRCi005-A and FAMRCi005-B) from patient carrying genetic variant LMNA p.Asp357Val
    Authors: P Klauzen, K Perepelina, A Khudiakov, A Zlotina, Y Fomicheva, T Pervunina, T Vershinina, A Kostareva, A Malashiche
    Stem Cell Res, 2020;43(0):101719.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Generation of two iPSC lines (FAMRCi004-A and FAMRCi004-B) from patient with familial progressive cardiac conduction disorder carrying genetic variant DSP p.His1684Arg
    Authors: A Khudiakov, K Perepelina, P Klauzen, A Zlotina, K Gusev, E Kaznacheye, A Malashiche, A Kostareva
    Stem Cell Res, 2020;43(0):101720.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Grafted human induced pluripotent stem cells improve the outcome of spinal cord injury: modulation of the lesion microenvironment
    Authors: T Bellák, Z Fekécs, D Török, Z Táncos, C Nemes, Z Tézsla, L Gál, S Polgári, J Kobolák, A Dinnyés, A Nógrádi, K Pajer
    Scientific Reports, 2020;10(1):22414.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Generation of two iPSC lines (FAMRCi006-A and FAMRCi006-B) from patient with dilated cardiomyopathy and Emery-Dreifuss muscular dystrophy associated with genetic variant LMNAp.Arg527Pro
    Authors: K Perepelina, P Klauzen, A Khudiakov, A Zlotina, Y Fomicheva, D Rudenko, M Gordeev, A Sergushich, A Malashiche, A Kostareva
    Stem Cell Res, 2020;43(0):101714.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Generation of two iPSC lines (FAMRCi007-A and FAMRCi007-B) from patient with Emery-Dreifuss muscular dystrophy and heart rhythm abnormalities carrying genetic variant LMNA p.Arg249Gln
    Authors: K Perepelina, A Kostina, P Klauzen, A Khudiakov, M Rabino, S Crasto, A Zlotina, Y Fomicheva, A Sergushich, M Oganesian, A Dmitriev, A Kostareva, E Di Pasqual, A Malashiche
    Stem Cell Res, 2020;47(0):101895.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Activation of PDGF pathway links LMNA mutation to dilated cardiomyopathy
    Authors: J Lee, V Termglinch, S Diecke, I Itzhaki, CK Lam, P Garg, E Lau, M Greenhaw, T Seeger, H Wu, JZ Zhang, X Chen, IP Gil, M Ameen, K Sallam, JW Rhee, JM Churko, R Chaudhary, T Chour, PJ Wang, MP Snyder, HY Chang, I Karakikes, JC Wu
    Nature, 2019;0(0):.
  12. Ciliary gene RPGRIP1L is required for hypothalamic arcuate neuron development
    Authors: L Wang, AJ De Solis, Y Goffer, KE Birkenbach, SE Engle, R Tanis, JM Levenson, X Li, R Rausch, M Purohit, JY Lee, J Tan, MC De Rosa, CA Doege, HL Aaron, GJ Martins, JC Brüning, D Egli, R Costa, N Berbari, RL Leibel, G Stratigopo
    JCI Insight, 2019;4(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Effects of the Extracts from Fruit and Stem of Camellia japonica on Induced Pluripotency and Wound Healing
    Authors: H Jeon, JY Kim, JK Choi, E Han, CL Song, J Lee, YS Cho
    J Clin Med, 2018;7(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. Small non-coding RNA landscape of extracellular vesicles from human stem cells
    Authors: S Kaur, AG Abu-Shahba, RO Paananen, H Hongisto, H Hiidenmaa, H Skottman, R Seppänen-K, B Mannerströ
    Sci Rep, 2018;8(1):15503.
    Species: Human
    Sample Types: Whole Cells
    Applications: Differentiation
  15. DMSO increases efficiency of genome editing at two non-coding loci
    Authors: G Stratigopo, MC De Rosa, CA LeDuc, RL Leibel, CA Doege
    PLoS ONE, 2018;13(6):e0198637.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
    Authors: PA De Sousa, R Steeg, E Wachter, K Bruce, J King, M Hoeve, S Khadun, G McConnachi, J Holder, A Kurtz, S Seltmann, J Dewender, S Reimann, G Stacey, O O'Shea, C Chapman, L Healy, H Zimmermann, B Bolton, T Rawat, I Atkin, A Veiga, B Kuebler, BM Serano, T Saric, J Hescheler, O Brüstle, M Peitz, C Thiele, N Geijsen, B Holst, C Clausen, M Lako, L Armstrong, SK Gupta, AJ Kvist, R Hicks, A Jonebring, G Brolén, A Ebneth, A Cabrera-So, P Foerch, M Geraerts, TC Stummann, S Harmon, C George, I Streeter, L Clarke, H Parkinson, PW Harrison, A Faulconbri, L Cherubin, T Burdett, C Trigueros, MJ Patel, C Lucas, B Hardy, R Predan, J Dokler, M Brajnik, O Keminer, O Pless, P Gribbon, C Claussen, A Ringwald, B Kreisel, A Courtney, TE Allsopp
    Stem Cell Res, 2017;20(0):105-114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. A Comprehensive TALEN-Based Knockout Library for Generating Human Induced Pluripotent Stem Cell-Based Models for Cardiovascular Diseases
    Authors: I Karakikes, V Termglinch, DA Cepeda, J Lee, S Diecke, A Hendel, I Itzhaki, M Ameen, R Shrestha, H Wu, N Ma, NY Shao, T Seeger, NA Woo, KD Wilson, E Matsa, MH Porteus, V Sebastiano, JC Wu
    Circ. Res, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. The effects of glomerular and tubular renal progenitors and derived extracellular vesicles on recovery from acute kidney injury
    Authors: A Ranghino, S Bruno, B Bussolati, A Moggio, V Dimuccio, M Tapparo, L Biancone, P Gontero, B Frea, G Camussi
    Stem Cell Res Ther, 2017;8(1):24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Generation of iPSC line from desmin-related cardiomyopathy patient carrying splice site mutation of DES gene
    Authors: A Khudiakov, D Kostina, A Zlotina, T Nikulina, A Sergushich, A Gudkova, A Tomilin, A Malashiche, A Kostareva
    Stem Cell Res, 2017;24(0):77-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Effect of microgravity on the mesenchymal stem cell characteristics of limbal fibroblasts
    Authors: SI Pao, KH Chien, HT Lin, MC Tai, JT Chen, CM Liang
    J Chin Med Assoc, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. High expression FUT1 and B3GALT5 is an independent predictor of postoperative recurrence and survival in hepatocellular carcinoma
    Authors: HH Kuo, RJ Lin, JT Hung, CB Hsieh, TH Hung, FY Lo, MY Ho, CT Yeh, YL Huang, J Yu, AL Yu
    Sci Rep, 2017;7(1):10750.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  22. Alloimmune Responses of Humanized Mice to Human Pluripotent Stem Cell Therapeutics
    Authors: NG Kooreman, PE de Almeida, JP Stack, RV Nelakanti, S Diecke, NY Shao, RJ Swijnenbur, V Sanchez-Fr, E Matsa, C Liu, AJ Connolly, JF Hamming, PHA Quax, MA Brehm, DL Greiner, LD Shultz, JC Wu
    Cell Rep, 2017;20(8):1978-1990.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Modeling Glanzmann thrombasthenia using patient specific iPSCs and restoring platelet aggregation function by CD41 overexpression
    Authors: L Hu, L Du, Y Zhao, W Li, Q Ouyang, D Zhou, G Lu, G Lin
    Stem Cell Res, 2017;20(0):14-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  24. A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity
    Authors: O Guillevic, S Ferratge, J Pascaud, C Driancourt, J Boyer-Di-P, G Uzan
    PLoS ONE, 2016;11(4):e0152993.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  25. Characterization and molecular profiling of PSEN1 familial Alzheimer's disease iPSC-derived neural progenitors.
    Authors: Sproul A, Jacob S, Pre D, Kim S, Nestor M, Navarro-Sobrino M, Santa-Maria I, Zimmer M, Aubry S, Steele J, Kahler D, Dranovsky A, Arancio O, Crary J, Gandy S, Noggle S
    PLoS ONE, 2014;9(1):e84547.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Telomerase-mediated telomere elongation from human blastocysts to embryonic stem cells.
    Authors: Zeng S, Liu L, Sun Y, Xie P, Hu L, Yuan D, Chen D, Ouyang Q, Lin G, Lu G
    J Cell Sci, 2014;127(0):752-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  27. Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells.
    Authors: Luo, Lan, Kawakatsu, Miho, Guo, Chao-Wan, Urata, Yoshishi, Huang, Wen-Jing, Ali, Haytham, Doi, Hanako, Kitajima, Yuriko, Tanaka, Takayuki, Goto, Shinji, Ono, Yusuke, Xin, Hong-Bo, Hamano, Kimikazu, Li, Tao-Shen
    Sci Rep, 2014;4(0):3779.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  28. Human dermis harbors distinct mesenchymal stromal cell subsets.
    Authors: Vaculik C, Schuster C, Bauer W, Iram N, Pfisterer K, Kramer G, Reinisch A, Strunk D, Elbe-Burger A
    J. Invest. Dermatol., 2012;132(3):563-74.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  29. Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
    Authors: Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM
    Stem Cells Dev., 2012;21(3):404-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells.
    Authors: Kim SY, Kim MJ, Jung H
    Stem Cells Dev., 2012;21(8):1272-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  31. Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
    BMC Vet Res, 2012;8(0):150.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium.
    Authors: Hayashi, Ryuhei, Ishikawa, Yuki, Ito, Miyuki, Kageyama, Tomofumi, Takashiba, Kuniko, Fujioka, Tsuyoshi, Tsujikawa, Motokazu, Miyoshi, Hiroyuki, Yamato, Masayuki, Nakamura, Yukio, Nishida, Kohji
    PLoS ONE, 2012;7(9):e45435.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  33. Generation of HIV-1 resistant and functional macrophages from hematopoietic stem cell-derived induced pluripotent stem cells.
    Authors: Kambal A, Mitchell G, Cary W, Gruenloh W, Jung Y, Kalomoiris S, Nacey C, McGee J, Lindsey M, Fury B, Bauer G, Nolta JA, Anderson JS
    Mol. Ther., 2011;19(3):584-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  34. A bioinformatic assay for pluripotency in human cells.
    Authors: Muller FJ, Schuldt BM, Williams R, Mason D, Altun G, Papapetrou EP, Danner S, Goldmann JE, Herbst A, Schmidt NO, Aldenhoff JB, Laurent LC, Loring JF
    Nat. Methods, 2011;8(4):315-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  35. Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
    Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH
    FEBS Lett., 2009;583(3):561-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  36. Tumor initiating potential of side population cells in human gastric cancer.
    Authors: Fukuda K, Saikawa Y, Ohashi M, Kumagai K, Kitajima M, Okano H, Matsuzaki Y, Kitagawa Y
    Int. J. Oncol., 2009;34(5):1201-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  37. Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells.
    Authors: Fong H, Hohenstein KA, Donovan PJ
    Stem Cells, 2008;26(8):1931-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  38. Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.
    Authors: Reed SA, Johnson SE
    J. Cell. Physiol., 2008;215(2):329-36.
    Species: Equine
    Sample Types: Whole Cells
    Applications: ICC
  39. Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells.
    Authors: Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ
    Stem Cells, 2008;26(6):1436-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  40. Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.
    Authors: Josephson R, Ording CJ, Liu Y, Shin S, Lakshmipathy U, Toumadje A, Love B, Chesnut JD, Andrews PW, Rao MS, Auerbach JM
    Stem Cells, 2007;25(2):437-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  41. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
    Authors: Rajesh&lt;/LastName&gt;&lt;ForeNam D&lt;/Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
    Stem Cells, 2007;25(2):490-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  42. Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells.
    Authors: Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH
    FEBS Lett., 2006;580(25):5869-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human/Mouse SSEA-4 Antibody

Average Rating: 4.2 (Based on 9 Reviews)

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Human/Mouse SSEA-4 Antibody
By Anonymous on 08/23/2021
Application: Flow Sample Tested: iPS2 human induced pluripotent stem cells Species: Human

Human/Mouse SSEA-4 Antibody
By Anonymous on 08/17/2021
Application: ICC/IF Sample Tested: differentiated corneal epithelial cells Species: Mouse

1:200 dilution worked well.


Human/Mouse SSEA-4 Antibody
By Julieta O'Flaherty on 12/11/2020
Application: ICC/IF Sample Tested: iPS2 human induced pluripotent stem cells Species: Human

I used to check for SSEA-4 in my iPSCs and it worked great


Human/Mouse SSEA-4 Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse SSEA-4 Antibody
By Anonymous on 11/20/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Human/Mouse SSEA-4 Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse SSEA-4 Antibody
By Anonymous on 11/07/2018
Application: ELISA Sample Tested: Plasma,Serum Species: Human and Mouse

Human/Mouse SSEA-4 Antibody
By Anonymous on 03/12/2018
Application: ICC/IF Sample Tested: fibrotic liver Species: Human

Human/Mouse SSEA-4 Antibody
By Simon Liu on 11/23/2017
Application: ICC/IF Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human