Human/Mouse SSEA-4 Antibody

Catalog # Availability Size / Price Qty
MAB1435
MAB1435-SP
Human/Mouse SSEA-4 Antibody in Data
2 Images
Product Details
Citations (33)
FAQs
Supplemental Products
Reviews (7)

Human/Mouse SSEA-4 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
Source
Monoclonal Mouse IgG3 Clone # MC-813-70
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
2120Ep human embryonal carcinoma cell line
Formulation
Lyophilized from a 0.2 μm filtered solution in MES with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of SSEA‑4 in NTera‑2 Human Cell Line by Flow Cytometry. View Larger

Detection of SSEA‑4 in NTera‑2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # MAB1435, filled histogram) or isotype control antibody (Catalog # MAB007, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Immunocytochemistry SSEA‑4 in BG01V Human Stem Cells. View Larger

SSEA‑4 in BG01V Human Stem Cells. SSEA-4 was detected in immersion fixed BG01V human embryonic stem cells cultured on irradiated mouse embryonic fibroblasts (Catalog # PSC001) using 10 µg/mL Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # MAB1435) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SSEA-4

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

References
  1. Shevinsky, L.H. et al. (1982) Cell 30:697.
  2. Kannagi, R. et al. (1983) EMBO J. 2:2355.
  3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
  4. Draper, J.S. et al. (2002) J. Anat. 200:249.
Long Name
Stage-specific Embryonic Antigen-4
Alternate Names
SSEA4; SSEA-4

Product Datasheets

Citations for Human/Mouse SSEA-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Activation of PDGF pathway links LMNA mutation to dilated cardiomyopathy
    Authors: J Lee, V Termglinch, S Diecke, I Itzhaki, CK Lam, P Garg, E Lau, M Greenhaw, T Seeger, H Wu, JZ Zhang, X Chen, IP Gil, M Ameen, K Sallam, JW Rhee, JM Churko, R Chaudhary, T Chour, PJ Wang, MP Snyder, HY Chang, I Karakikes, JC Wu
    Nature, 2019;0(0):.
  2. Ciliary gene RPGRIP1L is required for hypothalamic arcuate neuron development
    Authors: L Wang, AJ De Solis, Y Goffer, KE Birkenbach, SE Engle, R Tanis, JM Levenson, X Li, R Rausch, M Purohit, JY Lee, J Tan, MC De Rosa, CA Doege, HL Aaron, GJ Martins, JC Brüning, D Egli, R Costa, N Berbari, RL Leibel, G Stratigopo
    JCI Insight, 2019;4(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Effects of the Extracts from Fruit and Stem of Camellia japonica on Induced Pluripotency and Wound Healing
    Authors: H Jeon, JY Kim, JK Choi, E Han, CL Song, J Lee, YS Cho
    J Clin Med, 2018;7(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Small non-coding RNA landscape of extracellular vesicles from human stem cells
    Authors: S Kaur, AG Abu-Shahba, RO Paananen, H Hongisto, H Hiidenmaa, H Skottman, R Seppänen-K, B Mannerströ
    Sci Rep, 2018;8(1):15503.
    Species: Human
    Sample Types: Whole Cells
    Applications: Differentiation
  5. DMSO increases efficiency of genome editing at two non-coding loci
    Authors: G Stratigopo, MC De Rosa, CA LeDuc, RL Leibel, CA Doege
    PLoS ONE, 2018;13(6):e0198637.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. A Comprehensive TALEN-Based Knockout Library for Generating Human Induced Pluripotent Stem Cell-Based Models for Cardiovascular Diseases
    Authors: I Karakikes, V Termglinch, DA Cepeda, J Lee, S Diecke, A Hendel, I Itzhaki, M Ameen, R Shrestha, H Wu, N Ma, NY Shao, T Seeger, NA Woo, KD Wilson, E Matsa, MH Porteus, V Sebastiano, JC Wu
    Circ. Res, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Modeling Glanzmann thrombasthenia using patient specific iPSCs and restoring platelet aggregation function by CD41 overexpression
    Authors: L Hu, L Du, Y Zhao, W Li, Q Ouyang, D Zhou, G Lu, G Lin
    Stem Cell Res, 2017;20(0):14-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
    Authors: PA De Sousa, R Steeg, E Wachter, K Bruce, J King, M Hoeve, S Khadun, G McConnachi, J Holder, A Kurtz, S Seltmann, J Dewender, S Reimann, G Stacey, O O'Shea, C Chapman, L Healy, H Zimmermann, B Bolton, T Rawat, I Atkin, A Veiga, B Kuebler, BM Serano, T Saric, J Hescheler, O Brüstle, M Peitz, C Thiele, N Geijsen, B Holst, C Clausen, M Lako, L Armstrong, SK Gupta, AJ Kvist, R Hicks, A Jonebring, G Brolén, A Ebneth, A Cabrera-So, P Foerch, M Geraerts, TC Stummann, S Harmon, C George, I Streeter, L Clarke, H Parkinson, PW Harrison, A Faulconbri, L Cherubin, T Burdett, C Trigueros, MJ Patel, C Lucas, B Hardy, R Predan, J Dokler, M Brajnik, O Keminer, O Pless, P Gribbon, C Claussen, A Ringwald, B Kreisel, A Courtney, TE Allsopp
    Stem Cell Res, 2017;20(0):105-114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. High expression FUT1 and B3GALT5 is an independent predictor of postoperative recurrence and survival in hepatocellular carcinoma
    Authors: HH Kuo, RJ Lin, JT Hung, CB Hsieh, TH Hung, FY Lo, MY Ho, CT Yeh, YL Huang, J Yu, AL Yu
    Sci Rep, 2017;7(1):10750.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. Alloimmune Responses of Humanized Mice to Human Pluripotent Stem Cell Therapeutics
    Authors: NG Kooreman, PE de Almeida, JP Stack, RV Nelakanti, S Diecke, NY Shao, RJ Swijnenbur, V Sanchez-Fr, E Matsa, C Liu, AJ Connolly, JF Hamming, PHA Quax, MA Brehm, DL Greiner, LD Shultz, JC Wu
    Cell Rep, 2017;20(8):1978-1990.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Effect of microgravity on the mesenchymal stem cell characteristics of limbal fibroblasts
    Authors: SI Pao, KH Chien, HT Lin, MC Tai, JT Chen, CM Liang
    J Chin Med Assoc, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Generation of iPSC line from desmin-related cardiomyopathy patient carrying splice site mutation of DES gene
    Authors: A Khudiakov, D Kostina, A Zlotina, T Nikulina, A Sergushich, A Gudkova, A Tomilin, A Malashiche, A Kostareva
    Stem Cell Res, 2017;24(0):77-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. The effects of glomerular and tubular renal progenitors and derived extracellular vesicles on recovery from acute kidney injury
    Authors: A Ranghino, S Bruno, B Bussolati, A Moggio, V Dimuccio, M Tapparo, L Biancone, P Gontero, B Frea, G Camussi
    Stem Cell Res Ther, 2017;8(1):24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity
    Authors: O Guillevic, S Ferratge, J Pascaud, C Driancourt, J Boyer-Di-P, G Uzan
    PLoS ONE, 2016;11(4):e0152993.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  15. Characterization and molecular profiling of PSEN1 familial Alzheimer's disease iPSC-derived neural progenitors.
    Authors: Sproul A, Jacob S, Pre D, Kim S, Nestor M, Navarro-Sobrino M, Santa-Maria I, Zimmer M, Aubry S, Steele J, Kahler D, Dranovsky A, Arancio O, Crary J, Gandy S, Noggle S
    PLoS ONE, 2014;9(1):e84547.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Telomerase-mediated telomere elongation from human blastocysts to embryonic stem cells.
    Authors: Zeng S, Liu L, Sun Y, Xie P, Hu L, Yuan D, Chen D, Ouyang Q, Lin G, Lu G
    J Cell Sci, 2014;127(0):752-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells.
    Authors: Luo, Lan, Kawakatsu, Miho, Guo, Chao-Wan, Urata, Yoshishi, Huang, Wen-Jing, Ali, Haytham, Doi, Hanako, Kitajima, Yuriko, Tanaka, Takayuki, Goto, Shinji, Ono, Yusuke, Xin, Hong-Bo, Hamano, Kimikazu, Li, Tao-Shen
    Sci Rep, 2014;4(0):3779.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
    BMC Vet Res, 2012;8(0):150.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells.
    Authors: Kim SY, Kim MJ, Jung H
    Stem Cells Dev., 2012;21(8):1272-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium.
    Authors: Hayashi, Ryuhei, Ishikawa, Yuki, Ito, Miyuki, Kageyama, Tomofumi, Takashiba, Kuniko, Fujioka, Tsuyoshi, Tsujikawa, Motokazu, Miyoshi, Hiroyuki, Yamato, Masayuki, Nakamura, Yukio, Nishida, Kohji
    PLoS ONE, 2012;7(9):e45435.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
    Authors: Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM
    Stem Cells Dev., 2012;21(3):404-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Human dermis harbors distinct mesenchymal stromal cell subsets.
    Authors: Vaculik C, Schuster C, Bauer W, Iram N, Pfisterer K, Kramer G, Reinisch A, Strunk D, Elbe-Burger A
    J. Invest. Dermatol., 2012;132(3):563-74.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  23. Generation of HIV-1 resistant and functional macrophages from hematopoietic stem cell-derived induced pluripotent stem cells.
    Authors: Kambal A, Mitchell G, Cary W, Gruenloh W, Jung Y, Kalomoiris S, Nacey C, McGee J, Lindsey M, Fury B, Bauer G, Nolta JA, Anderson JS
    Mol. Ther., 2011;19(3):584-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  24. A bioinformatic assay for pluripotency in human cells.
    Authors: Muller FJ, Schuldt BM, Williams R, Mason D, Altun G, Papapetrou EP, Danner S, Goldmann JE, Herbst A, Schmidt NO, Aldenhoff JB, Laurent LC, Loring JF
    Nat. Methods, 2011;8(4):315-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  25. Tumor initiating potential of side population cells in human gastric cancer.
    Authors: Fukuda K, Saikawa Y, Ohashi M, Kumagai K, Kitajima M, Okano H, Matsuzaki Y, Kitagawa Y
    Int. J. Oncol., 2009;34(5):1201-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
    Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH
    FEBS Lett., 2009;583(3):561-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  27. Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells.
    Authors: Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ
    Stem Cells, 2008;26(6):1436-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  28. Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells.
    Authors: Fong H, Hohenstein KA, Donovan PJ
    Stem Cells, 2008;26(8):1931-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  29. Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.
    Authors: Reed SA, Johnson SE
    J. Cell. Physiol., 2008;215(2):329-36.
    Species: Equine
    Sample Types: Whole Cells
    Applications: ICC
  30. Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.
    Authors: Josephson R, Ording CJ, Liu Y, Shin S, Lakshmipathy U, Toumadje A, Love B, Chesnut JD, Andrews PW, Rao MS, Auerbach JM
    Stem Cells, 2007;25(2):437-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
    Authors: Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
    Stem Cells, 2007;25(2):490-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells.
    Authors: Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH
    FEBS Lett., 2006;580(25):5869-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse SSEA-4 Antibody

Average Rating: 4 (Based on 7 Reviews)

5 Star
28.57%
4 Star
42.86%
3 Star
28.57%
2 Star
0%
1 Star
0%

Have you used Human/Mouse SSEA-4 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse SSEA-4 Antibody
By Anonymous on 12/11/2020
Application: ICC/IF Sample Tested: iPS2 human induced pluripotent stem cells Species: Human

I used to check for SSEA-4 in my iPSCs and it worked great


Human/Mouse SSEA-4 Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse SSEA-4 Antibody
By Anonymous on 11/20/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Human/Mouse SSEA-4 Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse SSEA-4 Antibody
By Anonymous on 11/07/2018
Application: ELISA Sample Tested: Serum,Plasma Species: Human,Mouse

Human/Mouse SSEA-4 Antibody
By Anonymous on 03/12/2018
Application: ICC/IF Sample Tested: fibrotic liver Species: Human

Human/Mouse SSEA-4 Antibody
By Anonymous on 11/23/2017
Application: ICC/IF Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human