Human/Mouse SSEA-4 Antibody

(25 citations)
(1 Review)
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
  • Source
    Monoclonal Mouse IgG3 Clone # MC-813-70
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    2120Ep human embryonal carcinoma cell line
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in MES with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of SSEA‑4 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA‑4 Monoclonal Antibody (Catalog # MAB1435, filled histogram) or isotype control antibody (Catalog # MAB007, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Immunocytochemistry
SSEA‑4 in BG01V Human Stem Cells. SSEA‑4 was detected in immersion fixed BG01V human embryonic stem cells cultured on irradiated mouse embryonic fibroblasts (Catalog # PSC001) using 10 µg/mL Mouse Anti-Human/Mouse SSEA‑4 Monoclonal Antibody (Catalog # MAB1435) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SSEA-4

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

  • References:
    1. Shevinsky, L.H. et al. (1982) Cell 30:697.
    2. Kannagi, R. et al. (1983) EMBO J. 2:2355.
    3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
    4. Draper, J.S. et al. (2002) J. Anat. 200:249.
  • Long Name:
    Stage-specific Embryonic Antigen-4
  • Alternate Names:
    SSEA4; SSEA-4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Effect of microgravity on the mesenchymal stem cell characteristics of limbal fibroblasts
    Authors: SI Pao, KH Chien, HT Lin, MC Tai, JT Chen, CM Liang
    J Chin Med Assoc, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Alloimmune Responses of Humanized Mice to Human Pluripotent Stem Cell Therapeutics
    Authors: NG Kooreman, PE de Almeida, JP Stack, RV Nelakanti, S Diecke, NY Shao, RJ Swijnenbur, V Sanchez-Fr, E Matsa, C Liu, AJ Connolly, JF Hamming, PHA Quax, MA Brehm, DL Greiner, LD Shultz, JC Wu
    Cell Rep, 2017;20(8):1978-1990.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. The effects of glomerular and tubular renal progenitors and derived extracellular vesicles on recovery from acute kidney injury
    Authors: A Ranghino, S Bruno, B Bussolati, A Moggio, V Dimuccio, M Tapparo, L Biancone, P Gontero, B Frea, G Camussi
    Stem Cell Res Ther, 2017;8(1):24.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
    Authors: PA De Sousa, R Steeg, E Wachter, K Bruce, J King, M Hoeve, S Khadun, G McConnachi, J Holder, A Kurtz, S Seltmann, J Dewender, S Reimann, G Stacey, O O'Shea, C Chapman, L Healy, H Zimmermann, B Bolton, T Rawat, I Atkin, A Veiga, B Kuebler, BM Serano, T Saric, J Hescheler, O Brüstle, M Peitz, C Thiele, N Geijsen, B Holst, C Clausen, M Lako, L Armstrong, SK Gupta, AJ Kvist, R Hicks, A Jonebring, G Brolén, A Ebneth, A Cabrera-So, P Foerch, M Geraerts, TC Stummann, S Harmon, C George, I Streeter, L Clarke, H Parkinson, PW Harrison, A Faulconbri, L Cherubin, T Burdett, C Trigueros, MJ Patel, C Lucas, B Hardy, R Predan, J Dokler, M Brajnik, O Keminer, O Pless, P Gribbon, C Claussen, A Ringwald, B Kreisel, A Courtney, TE Allsopp
    Stem Cell Res, 2017;20(0):105-114.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Modeling Glanzmann thrombasthenia using patient specific iPSCs and restoring platelet aggregation function by CD41 overexpression
    Authors: L Hu, L Du, Y Zhao, W Li, Q Ouyang, D Zhou, G Lu, G Lin
    Stem Cell Res, 2017;20(0):14-20.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  6. A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity
    Authors: O Guillevic, S Ferratge, J Pascaud, C Driancourt, J Boyer-Di-P, G Uzan
    PLoS ONE, 2016;11(4):e0152993.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Fresh
  7. Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells.
    Authors: Luo, Lan, Kawakatsu, Miho, Guo, Chao-Wan, Urata, Yoshishi, Huang, Wen-Jing, Ali, Haytham, Doi, Hanako, Kitajima, Yuriko, Tanaka, Takayuki, Goto, Shinji, Ono, Yusuke, Xin, Hong-Bo, Hamano, Kimikazu, Li, Tao-Shen
    Sci Rep, 2014;4(0):3779.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  8. Telomerase-mediated telomere elongation from human blastocysts to embryonic stem cells.
    Authors: Zeng S, Liu L, Sun Y, Xie P, Hu L, Yuan D, Chen D, Ouyang Q, Lin G, Lu G
    J Cell Sci, 2014;127(0):752-62.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. Characterization and molecular profiling of PSEN1 familial Alzheimer's disease iPSC-derived neural progenitors.
    Authors: Sproul A, Jacob S, Pre D, Kim S, Nestor M, Navarro-Sobrino M, Santa-Maria I, Zimmer M, Aubry S, Steele J, Kahler D, Dranovsky A, Arancio O, Crary J, Gandy S, Noggle S
    PLoS ONE, 2014;9(1):e84547.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  10. Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium.
    Authors: Hayashi, Ryuhei, Ishikawa, Yuki, Ito, Miyuki, Kageyama, Tomofumi, Takashiba, Kuniko, Fujioka, Tsuyoshi, Tsujikawa, Motokazu, Miyoshi, Hiroyuki, Yamato, Masayuki, Nakamura, Yukio, Nishida, Kohji
    PLoS ONE, 2012;7(9):e45435.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  11. Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells.
    Authors: Kim SY, Kim MJ, Jung H
    Stem Cells Dev., 2012;21(8):1272-86.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  12. Human dermis harbors distinct mesenchymal stromal cell subsets.
    Authors: Vaculik C, Schuster C, Bauer W, Iram N, Pfisterer K, Kramer G, Reinisch A, Strunk D, Elbe-Burger A
    J. Invest. Dermatol., 2012;132(3):563-74.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  13. Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
    Authors: Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM
    Stem Cells Dev., 2012;21(3):404-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  14. Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
    BMC Vet Res, 2012;8(0):150.
    Species: Canine
    Sample Type: Whole Cells
    Application: Flow
  15. A bioinformatic assay for pluripotency in human cells.
    Authors: Muller FJ, Schuldt BM, Williams R, Mason D, Altun G, Papapetrou EP, Danner S, Goldmann JE, Herbst A, Schmidt NO, Aldenhoff JB, Laurent LC, Loring JF
    Nat. Methods, 2011;8(4):315-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  16. Generation of HIV-1 resistant and functional macrophages from hematopoietic stem cell-derived induced pluripotent stem cells.
    Authors: Kambal A, Mitchell G, Cary W, Gruenloh W, Jung Y, Kalomoiris S, Nacey C, McGee J, Lindsey M, Fury B, Bauer G, Nolta JA, Anderson JS
    Mol. Ther., 2011;19(3):584-93.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  17. Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
    Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH
    FEBS Lett., 2009;583(3):561-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  18. Tumor initiating potential of side population cells in human gastric cancer.
    Authors: Fukuda K, Saikawa Y, Ohashi M, Kumagai K, Kitajima M, Okano H, Matsuzaki Y, Kitagawa Y
    Int. J. Oncol., 2009;34(5):1201-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  19. Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.
    Authors: Reed SA, Johnson SE
    J. Cell. Physiol., 2008;215(2):329-36.
    Species: Equine
    Sample Type: Whole Cells
    Application: ICC
  20. Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells.
    Authors: Fong H, Hohenstein KA, Donovan PJ
    Stem Cells, 2008;26(8):1931-8.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  21. Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells.
    Authors: Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ
    Stem Cells, 2008;26(6):1436-43.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  22. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
    Authors: Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
    Stem Cells, 2007;25(2):490-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  23. Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.
    Authors: Josephson R, Ording CJ, Liu Y, Shin S, Lakshmipathy U, Toumadje A, Love B, Chesnut JD, Andrews PW, Rao MS, Auerbach JM
    Stem Cells, 2007;25(2):437-46.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  24. Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells.
    Authors: Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH
    FEBS Lett., 2006;580(25):5869-74.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 25 Citations
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Average Rating: 3 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human/Mouse SSEA-4 Antibody MAB1435
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 ICC/IF Human Anonymous 11/23/2017
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Immunocytochemistry/Immunofluorescence Human/Mouse SSEA-4 Antibody MAB1435
Immunocytochemistry/Immunofluorescence: Human/Mouse SSEA-4 Antibody [MAB1435]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedHUVEC human umbilical vein endothelial cells
SpeciesHuman

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