Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Flow Cytometry

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG3 Clone # MC-813-70
View all SSEA-4 Primary Antibodies »

Product Specifications

Immunogen

2120Ep human embryonal carcinoma cell line

Specificity

Recognizes a carbohydrate epitope of SSEA-4 (1, 2).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Human/Mouse SSEA‑4 PE‑conjugated Antibody

Detection of SSEA-4 antibody in NTera-2 Human Cell Line antibody by Flow Cytometry.

Detection of SSEA-4 in NTera-2 Human Cell Line by Flow Cytometry.

NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 PE-conjugated Mono-clonal Antibody (Catalog # FAB1435P, filled histogram) or isotype control antibody (Catalog # IC007P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse SSEA‑4 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: NTera‑2 human testicular embryonic carcinoma cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SSEA-4

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

References

  1. Shevinsky, L.H. et al. (1982) Cell 30:697.
  2. Kannagi, R. et al. (1983) EMBO J. 2:2355.
  3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
  4. Draper, J.S. et al. (2002) J. Anat. 200:249.

Long Name

Stage-specific Embryonic Antigen-4

Alternate Names

SSEA4

Additional SSEA-4 Products

Product Documents for Human/Mouse SSEA‑4 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse SSEA‑4 PE‑conjugated Antibody

For research use only

Related Research Areas

Mesenchymal Stem Cell Markers

Citations for Human/Mouse SSEA‑4 PE‑conjugated Antibody

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