Human/Mouse SSEA-4 PE-conjugated Antibody

(9 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
  • Source
    Monoclonal Mouse IgG3 Clone # MC-813-70
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    2120Ep human embryonal carcinoma cell line
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of SSEA-4 in NTera-2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 PE-conjugated Mono­clonal Antibody (Catalog # FAB1435P, filled histogram) or isotype control antibody (Catalog # IC007P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SSEA-4

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

  • References:
    1. Shevinsky, L.H. et al. (1982) Cell 30:697.
    2. Kannagi, R. et al. (1983) EMBO J. 2:2355.
    3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
    4. Draper, J.S. et al. (2002) J. Anat. 200:249.
  • Long Name:
    Stage-specific Embryonic Antigen-4
  • Alternate Names:
    SSEA4; SSEA-4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. An alternative pluripotent state confers interspecies chimaeric competency.
    Authors: Wu J, Okamura D, Li M, Suzuki K, Luo C, Ma L, He Y, Li Z, Benner C, Tamura I, Krause M, Nery J, Du T, Zhang Z, Hishida T, Takahashi Y, Aizawa E, Kim N, Lajara J, Guillen P, Campistol J, Esteban C, Ross P, Saghatelian A, Ren B, Ecker J, Izpisua Belmonte J
    Nature, 2015;521(7552):316-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Brief report: benchmarking human pluripotent stem cell markers during differentiation into the three germ layers unveils a striking heterogeneity: all markers are not equal.
    Stem Cells, 2012;29(9):1469-74.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells.
    Gene Ther, 2012;19(10):1035-40.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. GMP-compliant isolation and large-scale expansion of bone marrow-derived MSC.
    Authors: Fekete N, Rojewski M, Furst D, Kreja L, Ignatius A, Dausend J, Schrezenmeier H
    PLoS ONE, 2012;7(8):e43255.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Human dermis harbors distinct mesenchymal stromal cell subsets.
    Authors: Vaculik C, Schuster C, Bauer W, Iram N, Pfisterer K, Kramer G, Reinisch A, Strunk D, Elbe-Burger A
    J. Invest. Dermatol., 2012;132(3):563-74.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome.
    Authors: Carvajal-Vergara X, Sevilla A, D'Souza SL
    Nature, 2010;465(7299):808-12.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Expression of an exogenous human Oct-4 promoter identifies tumor-initiating cells in osteosarcoma.
    Authors: Levings PP, McGarry SV, Currie TP, Nickerson DM, McClellan S, Ghivizzani SC, Steindler DA, Gibbs CP
    Cancer Res., 2009;69(14):5648-55.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  8. Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery.
    Authors: Kiprilov EN, Awan A, Desprat R, Velho M, Clement CA, Byskov AG, Andersen CY, Satir P, Bouhassira EE, Christensen ST, Hirsch RE
    J. Cell Biol., 2008;180(5):897-904.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. T lineage differentiation from human embryonic stem cells.
    Authors: Galic Z, Kitchen SG, Kacena A, Subramanian A, Burke B, Cortado R, Zack JA
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(31):11742-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG3 PE-conjugated Antibody

Ctrl IC007P
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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