Human/Mouse SSEA-4 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1435P-100
FAB1435P-025
Detection of SSEA-4 in NTera-2 Human Cell Line by Flow Cytometry.
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Product Details
Citations (11)
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Human/Mouse SSEA-4 PE-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
Source
Monoclonal Mouse IgG3 Clone # MC-813-70
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
2120Ep human embryonal carcinoma cell line
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of SSEA-4 in NTera-2 Human Cell Line by Flow Cytometry. View Larger

Detection of SSEA-4 in NTera-2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 PE-conjugated Mono­clonal Antibody (Catalog # FAB1435P, filled histogram) or isotype control antibody (Catalog # IC007P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SSEA-4

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

References
  1. Shevinsky, L.H. et al. (1982) Cell 30:697.
  2. Kannagi, R. et al. (1983) EMBO J. 2:2355.
  3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
  4. Draper, J.S. et al. (2002) J. Anat. 200:249.
Long Name
Stage-specific Embryonic Antigen-4
Alternate Names
SSEA4; SSEA-4

Product Datasheets

Citations for Human/Mouse SSEA-4 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. A Marfan syndrome human induced pluripotent stem cell line with a heterozygous FBN1 c.4082G>A mutation, ISMMSi002-B, for disease modeling
    Authors: S Klein, JL Dvornik, AR Yarrabothu, C Schaniel
    Stem Cell Res, 2017;23(0):73-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. An alternative pluripotent state confers interspecies chimaeric competency.
    Authors: Wu J, Okamura D, Li M, Suzuki K, Luo C, Ma L, He Y, Li Z, Benner C, Tamura I, Krause M, Nery J, Du T, Zhang Z, Hishida T, Takahashi Y, Aizawa E, Kim N, Lajara J, Guillen P, Campistol J, Esteban C, Ross P, Saghatelian A, Ren B, Ecker J, Izpisua Belmonte J
    Nature, 2015;521(7552):316-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  3. Human dermis harbors distinct mesenchymal stromal cell subsets.
    Authors: Vaculik C, Schuster C, Bauer W, Iram N, Pfisterer K, Kramer G, Reinisch A, Strunk D, Elbe-Burger A
    J. Invest. Dermatol., 2012;132(3):563-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  4. Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells.
    Gene Ther, 2012;19(10):1035-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  5. GMP-compliant isolation and large-scale expansion of bone marrow-derived MSC.
    Authors: Fekete N, Rojewski M, Furst D, Kreja L, Ignatius A, Dausend J, Schrezenmeier H
    PLoS ONE, 2012;7(8):e43255.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  6. Brief report: benchmarking human pluripotent stem cell markers during differentiation into the three germ layers unveils a striking heterogeneity: all markers are not equal.
    Stem Cells, 2012;29(9):1469-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  7. Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome.
    Authors: Carvajal-Vergara X, Sevilla A, D'Souza SL
    Nature, 2010;465(7299):808-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  8. Expression of an exogenous human Oct-4 promoter identifies tumor-initiating cells in osteosarcoma.
    Authors: Levings PP, McGarry SV, Currie TP, Nickerson DM, McClellan S, Ghivizzani SC, Steindler DA, Gibbs CP
    Cancer Res., 2009;69(14):5648-55.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  9. Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery.
    Authors: Kiprilov EN, Awan A, Desprat R, Velho M, Clement CA, Byskov AG, Andersen CY, Satir P, Bouhassira EE, Christensen ST, Hirsch RE
    J. Cell Biol., 2008;180(5):897-904.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. T lineage differentiation from human embryonic stem cells.
    Authors: Galic Z, Kitchen SG, Kacena A, Subramanian A, Burke B, Cortado R, Zack JA
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(31):11742-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  11. Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation.
    Authors: Semenov O, Koestenbauer S, Riegel M, Zech N, Zimmermann R, Zisch A, Malek A
    Am J Obstet Gynecol, 0;202(2):193.e1-193.e1.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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