Human/Mouse STAT4 Antibody

Catalog # Availability Size / Price Qty
PAF-ST4
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human/Mouse STAT4 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse STAT4. No cross-reactivity with the other STATs has been detected by immunoprecipitation or by Western blotting.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse STAT4 synthetic peptide
aa 675-749
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
Human peripheral blood lymphocytes
Immunoprecipitation
1 µg/106 cells
Human peripheral blood lymphocytes, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT4

Signal transduction and activator of transcription 4 (STAT4) is a member of the Jak/STAT signal transduction pathway. STAT4 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, homo or heterodimerizes with other STAT proteins, which then translocate to the nucleus and activate transcription of specific genes. STAT4 is expressed in subset of tissues including spleen, heart, brain, peripheral blood cells and testis. STAT4 is involved in mediating IL-12 response in lymphocytes and regulating T helper cell differentiation.

Long Name
Signal Transducer and Activator of Transcription 4
Entrez Gene IDs
6775 (Human); 20849 (Mouse)
Alternate Names
signal transducer and activator of transcription 4; SLEB11; STAT4

Product Datasheets

Citation for Human/Mouse STAT4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Leukemia inhibitory factor is linked to regulatory transplantation tolerance.
    Authors: Metcalfe SM, Watson TJ, Shurey S, Adams E, Green CJ
    Transplantation, 2005;79(6):726-30.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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