Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all STAT4 Primary Antibodies »

Product Specifications

Immunogen

Mouse STAT4 synthetic peptide
aa 675-749

Specificity

Detects human and mouse STAT4. No cross-reactivity with the other STATs has been detected by immunoprecipitation or by Western blotting.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human/Mouse STAT4 Antibody

Application
Recommended Usage

Immunoprecipitation

1 µg/106 cells
Sample: Human peripheral blood lymphocytes, see our available Western blot detection antibodies

Western Blot

0.2 µg/mL
Sample: Human peripheral blood lymphocytes

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STAT4

Signal transduction and activator of transcription 4 (STAT4) is a member of the Jak/STAT signal transduction pathway. STAT4 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, homo or heterodimerizes with other STAT proteins, which then translocate to the nucleus and activate transcription of specific genes. STAT4 is expressed in subset of tissues including spleen, heart, brain, peripheral blood cells and testis. STAT4 is involved in mediating IL-12 response in lymphocytes and regulating T helper cell differentiation.

Long Name

Signal Transducer and Activator of Transcription 4

Alternate Names

signal transducer and activator of transcription 4, SLEB11

Entrez Gene IDs

6775 (Human); 20849 (Mouse)

Gene Symbol

STAT4

Additional STAT4 Products

Product Documents for Human/Mouse STAT4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse STAT4 Antibody

For research use only

Citations for Human/Mouse STAT4 Antibody

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FAQs

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