Human/Mouse TREM-2 Alexa Fluor® 700-conjugated Antibody FAB17291N: R&D Systems

Human/Mouse TREM-2 Alexa Fluor® 700-conjugated Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse TREM-2 in direct ELISAs and Western blots. Stains TREM-2 transfectants but not TREM-1 transfectants.
  • Source
    Monoclonal Rat IgG2B Clone # 237920
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse TREM-2
    extracellular domain
    Accession # Q99NH8
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TREM‑2 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse TREM‑2 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB17291N, filled histogram) or isotype control antibody (Catalog # IC013N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of TREM‑2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human/Mouse TREM‑2 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB17291N, filled histogram) or isotype control antibody (Catalog # IC013N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of TREM‑2 in THP‑1 Human Cell Line by Flow Cytometry. THP‑1 human acute monocytic leukemia cell line was stained with Rat Anti-Human/Mouse TREM‑2 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB17291N, filled histogram) or isotype control antibody (Catalog # IC013N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of TREM‑2 in HEK Human Cell Line Transfected with Human TREM-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human TREM-2 or (B) human TREM-1 and eGFP was stained with Rat Anti-Human/Mouse TREM‑2 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB17291N). Quadrant markers were set based on control antibody staining (Catalog # IC013N). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TREM-2

TREM-2 is a single Ig domain receptor expressed by cells of myeloid lineage. Two closely related transcripts were reported in mouse and designated TREM-2a and TREM-2b. TREM-2b is a pattern recognition receptor that binds anionic ligands. TREM-2 associates with the adapter protein DAP12 to deliver an activating signal that plays a role in both innate and adaptive immune responses.

  • Long Name:
    Triggering Receptor Expressed on Myeloid Cells 2
  • Entrez Gene IDs:
    54209 (Human)
  • Alternate Names:
    TREM2; TREM-2; Trem2a; Trem2b; Trem2c; TREM-2triggering receptor expressed on myeloid cells 2a; Triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Alexa Fluor® 700-conjugated Antibody

Ctrl IC013N  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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