Human/Mouse UNC13A Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse UNC13A in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human UNC13A
    Asp1525-Gly1698
    Accession # Q9UPW8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse UNC13A by Western Blot. Western blot shows lysates of human brain (hippocampus) tissue and mouse brain tissue. PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse UNC13A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6214) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for UNC13A at approximately 190-210 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: UNC13A

UNC13A (UNCoordinated 13A; also Munc13-1) is a 190‑210 kDa member of the UNC-13 family, C2 domain protein superfamily of molecules. It is expressed at synaptic junctions, particularly those involved in GABA, acetylcholine and glutamine transmission, and appears to be necessary for neurotransmitter release. In the presynaptic region, UNC13A likely interacts with syntaxin 1, converting it from a closed to an open configuration, thus allowing for SNARE complex formation. Human UNC13A is 1703 amino acids (aa) in length. It contains one C2 domain that mediates dimerization (aa 1‑79), a coiled‑coil region (aa 320‑357), one DAG Zn‑finger region (aa 553‑603), a second C2 domain (aa 663‑769), two MHD domains (aa 1093‑1512) and a third C2 domain (aa 1532‑1637). Western blots of mouse UNC13A show 105 kDa and 70 kDa forms. Over aa 1525‑1698, human UNC13A shares 98% aa identity with mouse UNC13A.

  • Long Name:
    UNC-13 homolog A
  • Entrez Gene IDs:
    23025 (Human); 382018 (Mouse); 64829 (Rat)
  • Alternate Names:
    KIAA1032Munc13-1protein unc-13 homolog A; Munc13-1; unc-13 homolog A (C. elegans); UNC13A
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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