ZIC3 Antibody

R&D Systems | Catalog # AF5310

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ZIC3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ZIC3
His380-Val467
Accession # O60481

Specificity

Detects endogenous human and mouse ZIC3 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for ZIC3 Antibody

Detection of Human/Mouse ZIC3 antibody by Western Blot.

Detection of Human/Mouse ZIC3 by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse ZIC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5310) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ZIC3 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for ZIC3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line

Reviewed Applications

Read 1 review rated 4 using AF5310 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ZIC3

ZIC3 (zinc-finger protein of the cerebellum 3) is a 50 kDa (predicted) member of the GLI C2H2-type Zn-finger protein family. It is expressed in cerebellum, and apparently plays a key role in left-right body axis formation. Human ZIC3 is 467 amino acids (aa) in length. It contains N‑terminal poly-Ala (aa 46‑55) and poly-His (aa 87‑97) segments, followed by five C2H2-type Zn-fingers (aa 253‑410). Within the zinc finger region, there is an overlapping NES (aa 290‑349) and two NLS's (aa 367‑382 and 403‑410). ZIC3 reportedly binds to GLI-3. There is one potential splice form that shows a 49 aa substitution for aa 409‑467, and a second splice form that shows a three aa addition to the C‑terminus. Over aa 380‑467, human ZIC3 displays absolute/100% identity to mouse ZIC3.

Long Name

Zic Family Member 3

Alternate Names

Heterotaxy 1, HTX1, ZNF203

Entrez Gene IDs

7547 (Human); 22773 (Mouse); 367944 (Rat)

Gene Symbol

ZIC3

UniProt

O60481

Additional ZIC3 Products

Product Documents for ZIC3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for ZIC3 Antibody

For research use only

Related Research Areas

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Customer Reviews for ZIC3 Antibody (1)

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  • Human/Mouse ZIC3 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/17/2021
    ZIC3 Antibody AF5310

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