Human Mre11 Antibody

Catalog # Availability Size / Price Qty
AF2284
AF2284-SP

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Detection of Human Mre11 by Western Blot.
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Product Details
Citations (1)
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Human Mre11 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Mre11 in Western blots.
Source
Polyclonal Chicken IgY
Purification
Antigen Affinity-purified from egg yolks
Immunogen
E. coli-derived recombinant human Mre11
Glu423-Arg708
Accession # P49959
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Mre11 antibody by Western Blot. View Larger

Detection of Human Mre11 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Human Mre11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2284) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. A specific band was detected for Mre11 at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

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Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mre11

The Mre11 protein exhibits both exo- and endonuclease activities and is a component of the Mre11/Rad50/Nbs1 (MRN) complex. The MRN complex binds chromatin in regions of DNA double-strand breaks (DSB) during the normal cell cycle and in response to genotoxic stress, and is involved in control of DSB repair during homologous recombination.

Long Name
Meiotic Recombination 11 Homolog A
Entrez Gene IDs
4361 (Human)
Alternate Names
ATLDmeiotic recombination (S. cerevisiae) 11 homolog A; AT-like disease; DNA recombination and repair protein; double-strand break repair protein MRE11A; endo/exonuclease Mre11; HNGS1; Meiotic recombination 11 homolog 1; Meiotic recombination 11 homolog A; MRE11 homolog 1; MRE11 homolog A; MRE11 meiotic recombination 11 homolog A (S. cerevisiae); Mre11; MRE11A; MRE11B

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Citation for Human Mre11 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. After double-strand break induction by UV-A, homologous recombination and nonhomologous end joining cooperate at the same DSB if both systems are available.
    Authors: Rapp A, Greulich KO
    J. Cell. Sci., 2004-09-14;117(0):4935-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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