Human Mre11 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Mre11 in Western blots.
  • Source
    Polyclonal Chicken IgY
  • Purification
    Antigen Affinity-purified from egg yolks
  • Immunogen
    E. coli-derived recombinant human Mre11
    Glu423-Arg708
    Accession # P49959
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Mre11 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Human Mre11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2284) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. A specific band was detected for Mre11 at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mre11

The Mre11 protein exhibits both exo- and endonuclease activities and is a component of the Mre11/Rad50/Nbs1 (MRN) complex. The MRN complex binds chromatin in regions of DNA double-strand breaks (DSB) during the normal cell cycle and in response to genotoxic stress, and is involved in control of DSB repair during homologous recombination.

  • Long Name:
    Meiotic Recombination 11 Homolog A
  • Entrez Gene IDs:
    4361 (Human)
  • Alternate Names:
    ATLDmeiotic recombination (S. cerevisiae) 11 homolog A; AT-like disease; DNA recombination and repair protein; double-strand break repair protein MRE11A; endo/exonuclease Mre11; HNGS1; Meiotic recombination 11 homolog 1; Meiotic recombination 11 homolog A; MRE11 homolog 1; MRE11 homolog A; MRE11 meiotic recombination 11 homolog A (S. cerevisiae); Mre11; MRE11A; MRE11MRE11B
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. After double-strand break induction by UV-A, homologous recombination and nonhomologous end joining cooperate at the same DSB if both systems are available.
    Authors: Rapp A, Greulich KO
    J. Cell. Sci., 2004;117(0):4935-45.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Isotype Controls
Description Application Cat# Citations Images  

Normal Chicken IgY Control

Ctrl AB-101-C 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat Anti-Chicken IgY NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL016 1
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Goat Anti-Chicken IgY NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL017 1
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Goat Anti-Chicken IgY Antibody

WB AF010 1
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Goat Anti-Chicken IgY NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL018
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Goat Anti-Chicken IgY Biotinylated Antibody

WB BAF010
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