The Mre11 protein exhibits both exo- and endonuclease activities and is a component of the Mre11/Rad50/Nbs1 (MRN) complex. The MRN complex binds chromatin in regions of DNA double-strand breaks (DSB) during the normal cell cycle and in response to genotoxic stress, and is involved in control of DSB repair during homologous recombination.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Chicken IgY
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Product Specifications
Immunogen
E. coli-derived recombinant human Mre11
Glu423-Arg708
Accession # P49959
Glu423-Arg708
Accession # P49959
Specificity
Detects human Mre11 in Western blots.
Clonality
Polyclonal
Host
Chicken
Isotype
IgY
Scientific Data Images for Human Mre11 Antibody
Detection of Human Mre11 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Human Mre11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2284) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. A specific band was detected for Mre11 at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Applications for Human Mre11 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line
Sample: HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified from egg yolks
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Mre11
Long Name
Meiotic Recombination 11 Homolog A
Alternate Names
MRE11A
Entrez Gene IDs
4361 (Human)
Gene Symbol
MRE11
UniProt
Additional Mre11 Products
Product Documents for Human Mre11 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Mre11 Antibody
For research use only
Citations for Human Mre11 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars