Human Nanog Antibody [NL493]

R&D Systems | Catalog # NL1997G

R&D Systems
Discontinued Product
NL1997G has been discontinued. View all Nanog products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry

Cited:

Flow Cytometry, Immunocytochemistry

Label

NL493 (Excitation = 493 nm, Emission = 514 nm)

Antibody Source

Polyclonal Goat IgG
View all Nanog Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Nanog

Specificity

Detects human Nanog in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Nanog is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Nanog Antibody [NL493]

Application
Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Nanog

Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1-3).

References

  1. Mitsui, K. et al. (2003) Cell 11:631.
  2. Chambers, I. et al. (2003) Cell 113:643.
  3. Hart, A.H. et al. (2004) Dev. Dyn. 230:187.

Long Name

Nanog Homeobox

Alternate Names

FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox

Entrez Gene IDs

79923 (Human); 71950 (Mouse)

Gene Symbol

NANOG

UniProt

ABZ92376

Additional Nanog Products

Product Documents for Human Nanog Antibody [NL493]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Nanog Antibody [NL493]

For research use only

Citations for Human Nanog Antibody [NL493]

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