Human Nanos2 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB2870N Datasheet / COA / SDS

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FAB2870N-100UG

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Human Nanos2 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Nanos2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Nanos1 is observed.

Source

Monoclonal Mouse IgG1 Clone # 343255

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Nanos2
Met1-Arg138
Accession # P60321

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Nanos2

Nanos2 is a cytoplasmic RNA-binding protein that belongs to the Nanos family of translational repressors. The 138 amino acid (aa) protein contains a 55 aa nanos‑type zinc finger domain with two C2HC motifs. Human and mouse Nanos2 share identity 75% aa sequence identity overall but 95% within the zinc finger RNA binding domain. In the mouse, Nanos2 is located in male germ cells and is essential for spermatogenesis.

Entrez Gene IDs

339345 (Human)

Alternate Names

nanos homolog 2 (Drosophila); nanos homolog 2; Nanos2; NOS2NOS-2