Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 343255
View all Nanos2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Nanos2
Met1-Arg138
Accession # P60321

Specificity

Detects human Nanos2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Nanos1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Nanos2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Nanos2

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nanos2

Nanos2 is a cytoplasmic RNA-binding protein that belongs to the Nanos family of translational repressors. The 138 amino acid (aa) protein contains a 55 aa nanos‑type zinc finger domain with two C2HC motifs. Human and mouse Nanos2 share identity 75% aa sequence identity overall but 95% within the zinc finger RNA binding domain. In the mouse, Nanos2 is located in male germ cells and is essential for spermatogenesis.

Alternate Names

nanos homolog 2, nanos homolog 2 (Drosophila), NOS2NOS-2

Entrez Gene IDs

339345 (Human)

Gene Symbol

NANOS2

UniProt

P60321

Additional Nanos2 Products

Product Documents for Human Nanos2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Nanos2 Antibody

For research use only

Related Research Areas

Primordial and Mature Germ Cells

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