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Human NEU-1/Sialidase-1 Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB6860X Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB6860X-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NEU-1/Sialidase-1 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human NEU-1/Sialidase-1 in direct ELISAs.
Source
Monoclonal Mouse IgG2b Clone # 688215
Purification
Protein A or G purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NEU-1/Sialidase-1
Lys46-Leu415
Accession # Q99519
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: NEU-1/Sialidase-1

Neuraminidase, also called NEU-1 or lysosomal sialidase, is a 45 kDa glycoprotein in the N-acetyl-a neuraminidase family. It is a member of a complex within the secretory lysosomes of melanosomes and hematopoietic cells that removes terminal sialic acids from glycosylated proteins and lipids. Mature human NEU-1 (aa 46‑415) shares 86.5% aa identity with mouse and rat NEU-1. A 225 aa splice variant shares aa 206‑415, but has an alternate N-terminus.

Long Name
N-acetyl-alpha-Neuraminidase 1
Entrez Gene IDs
4758 (Human); 18010 (Mouse); 24591 (Rat)
Alternate Names
Acetylneuraminyl hydrolase; EC 3.2.1.18; exo-alpha-sialidase; FLJ93471; G9 sialidase; Lysosomal sialidase; N-acetyl-alpha-neuraminidase 1; NANH; NEU; NEU1; NEU-1; SIAL1; sialidase 1 (lysosomal sialidase); Sialidase-1

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