Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 688215
View all NEU-1/Sialidase-1 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NEU-1/Sialidase-1
Lys46-Leu415
Accession # Q99519

Specificity

Detects human NEU-1/Sialidase-1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human NEU‑1/Sialidase‑1 Antibody

Detection of Human NEU‑1/Sialidase‑1 antibody by Western Blot.

Detection of Human NEU‑1/Sialidase‑1 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NEU-1/Sialidase-1 Monoclonal Antibody (Catalog # MAB6860) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NEU-1/Sialidase-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human NEU‑1/Sialidase‑1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human NEU-1/Sialidase-1, see our available Western blot detection antibodies

Western Blot

2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and human pancreas tissue

Reviewed Applications

Read 1 review rated 4 using MAB6860 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NEU-1/Sialidase-1

Neuraminidase, also called NEU-1 or lysosomal sialidase, is a 45 kDa glycoprotein in the N-acetyl-a neuraminidase family. It is a member of a complex within the secretory lysosomes of melanosomes and hematopoietic cells that removes terminal sialic acids from glycosylated proteins and lipids. Mature human NEU-1 (aa 46‑415) shares 86.5% aa identity with mouse and rat NEU-1. A 225 aa splice variant shares aa 206‑415, but has an alternate N-terminus.

Long Name

N-acetyl-alpha-Neuraminidase 1

Alternate Names

NEU1, Sialidase-1

Entrez Gene IDs

4758 (Human); 18010 (Mouse); 24591 (Rat)

Gene Symbol

NEU1

UniProt

Q99519

Additional NEU-1/Sialidase-1 Products

Product Documents for Human NEU‑1/Sialidase‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human NEU‑1/Sialidase‑1 Antibody

For research use only

Citations for Human NEU‑1/Sialidase‑1 Antibody

Customer Reviews for Human NEU‑1/Sialidase‑1 Antibody (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 24200502
    Species: Human
    Verified Customer | Posted 02/25/2015

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