Human NEU-1/Sialidase-1 Antibody Summary
Accession # Q99519
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human NEU‑1/Sialidase‑1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NEU-1/Sialidase-1 Monoclonal Antibody (Catalog # MAB6860) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NEU-1/Sialidase-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Neuraminidase, also called NEU-1 or lysosomal sialidase, is a 45 kDa glycoprotein in the N-acetyl-a neuraminidase family. It is a member of a complex within the secretory lysosomes of melanosomes and hematopoietic cells that removes terminal sialic acids from glycosylated proteins and lipids. Mature human NEU-1 (aa 46‑415) shares 86.5% aa identity with mouse and rat NEU-1. A 225 aa splice variant shares aa 206‑415, but has an alternate N-terminus.
Citation for Human NEU-1/Sialidase-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Sialic acid attenuates puromycin aminonucleoside-induced desialylation and oxidative stress in human podocytes.
Authors: Pawluczyk I, Ghaderi Najafabadi M, Patel S, Desai P, Vashi D, Saleem M, Topham P
Exp Cell Res, 2014;320(2):258-68.
Sample Types: Cell Lysates
Applications: Western Blot
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