Human NEU-1/Sialidase-1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human NEU‑1/Sialidase‑1 by Western Blot.
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Product Details
Citations (1)
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Human NEU-1/Sialidase-1 Antibody Summary

Species Reactivity
Detects human NEU-1/Sialidase-1 in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 688215
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NEU-1/Sialidase-1
Accession # Q99519
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human NEU-1/Sialidase-1, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human NEU‑1/Sialidase‑1 antibody by Western Blot. View Larger

Detection of Human NEU‑1/Sialidase‑1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NEU-1/Sialidase-1 Monoclonal Antibody (Catalog # MAB6860) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NEU-1/Sialidase-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NEU-1/Sialidase-1

Neuraminidase, also called NEU-1 or lysosomal sialidase, is a 45 kDa glycoprotein in the N-acetyl-a neuraminidase family. It is a member of a complex within the secretory lysosomes of melanosomes and hematopoietic cells that removes terminal sialic acids from glycosylated proteins and lipids. Mature human NEU-1 (aa 46‑415) shares 86.5% aa identity with mouse and rat NEU-1. A 225 aa splice variant shares aa 206‑415, but has an alternate N-terminus.

Long Name
N-acetyl-alpha-Neuraminidase 1
Entrez Gene IDs
4758 (Human); 18010 (Mouse); 24591 (Rat)
Alternate Names
Acetylneuraminyl hydrolase; EC; exo-alpha-sialidase; FLJ93471; G9 sialidase; Lysosomal sialidase; N-acetyl-alpha-neuraminidase 1; NANH; NEU; NEU1; NEU-1; SIAL1; sialidase 1 (lysosomal sialidase); Sialidase-1

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Citation for Human NEU-1/Sialidase-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Sialic acid attenuates puromycin aminonucleoside-induced desialylation and oxidative stress in human podocytes.
    Authors: Pawluczyk I, Ghaderi Najafabadi M, Patel S, Desai P, Vashi D, Saleem M, Topham P
    Exp Cell Res, 2014;320(2):258-68.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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