Human NFATC1 Antibody

Catalog # Availability Size / Price Qty
AF5640
AF5640-SP
Detection of Human NFATC1 by Western Blot.
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Product Details
Citations (1)
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Human NFATC1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NFATC1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human NFATC1
Ala48-Ser406
Accession # O95644
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human NFATC1 antibody by Western Blot. View Larger

Detection of Human NFATC1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Ramos human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human NFATC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5640) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for NFATC1 at approximately 110 - 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human NFATC1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human NFATC1 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for NFATC1 at approximately 124 kDa (as indicated) using 10 µg/mL of Goat Anti-Human NFATC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5640) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFATC1

NFATC1 (Nuclear factor of activated T cells c1; also NFAT2a) is a 120 kDa member of the NFAT family of transcription factors. NFATC1 is found in T cells and mast cells, and regulates cytokine transcription. NFATC1 is normally cytoplasmic and phosphorylated at Ser172. Upon a rise in intracellular Ca+2, dephosphorylation occurs via calcineurin, and NFATC1 enters the nucleus. Human NFATC1 is 943 amino acids (aa) in length. It contains a calcineurin-binding site (aa 118‑123), two transactivation domains (aa 126‑218 and 703‑943), two NLSs (aa 265‑267 and 682‑684), an NES (aa 310-321) and an RHD that binds DNA (aa 410‑592). There are multiple isoforms. Individually or in common, there is an alternate start site at Met473, a deletion of aa 826‑943, a 29 aa substitution for aa 1‑42, and a 19 aa substitution for aa 698‑943.

Long Name
Nuclear Factor of Activated T Cells C1
Entrez Gene IDs
4772 (Human); 18018 (Mouse)
Alternate Names
MGC138448; NFAT transcription complex cytosolic component; NFAT2; NFAT2cytoplasmic 1; NFATC; NF-ATC; NFATC1; NF-ATc1; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1

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Citation for Human NFATC1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Human venous valve disease caused by mutations in FOXC2 and GJC2
    Authors: Oliver Lyons, Prakash Saha, Christopher Seet, Adam Kuchta, Andrew Arnold, Steven Grover et al.
    Journal of Experimental Medicine

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