Human NFATC1 Antibody

R&D Systems | Catalog # AF5640

R&D Systems
Discontinued Product
AF5640 has been discontinued. View all NFATC1 products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NFATC1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NFATC1
Ala48-Ser406
Accession # O95644

Specificity

Detects human NFATC1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human NFATC1 Antibody

Detection of Human NFATC1 antibody by Western Blot.

Detection of Human NFATC1 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Ramos human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human NFATC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5640) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for NFATC1 at approximately 110 - 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human NFATC1 antibody by Simple WesternTM.

Detection of Human NFATC1 by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for NFATC1 at approximately 124 kDa (as indicated) using 10 µg/mL of Goat Anti-Human NFATC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5640) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human NFATC1 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, Ramos human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NFATC1

NFATC1 (Nuclear factor of activated T cells c1; also NFAT2a) is a 120 kDa member of the NFAT family of transcription factors. NFATC1 is found in T cells and mast cells, and regulates cytokine transcription. NFATC1 is normally cytoplasmic and phosphorylated at Ser172. Upon a rise in intracellular Ca+2, dephosphorylation occurs via calcineurin, and NFATC1 enters the nucleus. Human NFATC1 is 943 amino acids (aa) in length. It contains a calcineurin-binding site (aa 118‑123), two transactivation domains (aa 126‑218 and 703‑943), two NLSs (aa 265‑267 and 682‑684), an NES (aa 310-321) and an RHD that binds DNA (aa 410‑592). There are multiple isoforms. Individually or in common, there is an alternate start site at Met473, a deletion of aa 826‑943, a 29 aa substitution for aa 1‑42, and a 19 aa substitution for aa 698‑943.

Long Name

Nuclear Factor of Activated T Cells C1

Alternate Names

NFAT2

Entrez Gene IDs

4772 (Human); 18018 (Mouse)

Gene Symbol

NFATC1

UniProt

O95644

Additional NFATC1 Products

Product Documents for Human NFATC1 Antibody

Certificate of Analysis

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Product Specific Notices for Human NFATC1 Antibody

For research use only

Citations for Human NFATC1 Antibody

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