Goat IgG HRP-conjugated Antibody
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Summary
Data Examples
Preparation and Storage
Reconstitution Calculator
Background
Product Datasheets
Related Research Areas
Goat IgG HRP-conjugated Antibody Summary
Species Reactivity
Goat
Specificity
Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with human IgG, rabbit IgG, mouse IgG and less than 1% cross-reactivity with chicken IgY is observed.
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Goat IgG
Formulation
Supplied as a 0.2 um filtered solution in PBS with stabilizers.
Label
HRP (Horseradish Peroxidase)
Applications
Recommended Concentration
Sample
Western Blot
Use at a 1:1000 dilution.
Simple Western
Use at a 1:50 dilution.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Western Blot
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Detection of Goat IgGPrimary Antibody by Western Blot Western blot shows detection of Goat Anti-Human/Mouse/Rat GRP75/HSPA9BPolyclonal Antibody (Catalog # AF3584)using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody(Catalog # HAF109). This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 2.
Simple Western
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Detection of Goat IgG Primary Antibody by Simple WesternTM.
Simple Western lane view shows detection of Goat Anti-Human/Mouse/Rat TC-PTP Polyclonal Antibody (Catalog #AF1930) using a 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody
(Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Reconstitution Calculator
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
IgG
Product Datasheets
Citations for Goat IgG HRP-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Proteomics analysis of colon cancer progression
Authors: S Saleem, S Tariq, I Aleem, Sadr-Ul Sh, M Tahseen, A Atiq, S Hassan, M Abu Bakar, S Khattak, AA Syed, AH Ahmad, M Hussain, MA Yusuf, C Sutton
Clin Proteomics, 2019;16(0):44.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Induction of 3-hydroxy-3-methylglutaryl-CoA reductase mediates statin resistance in breast cancer cells
Authors: A Göbel, D Breining, M Rauner, LC Hofbauer, TD Rachner
Cell Death Dis, 2019;10(2):91.
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A Disintegrin and Metalloproteinase 9 Domain (ADAM9) Is a Major Susceptibility Factor in the Early Stages of Encephalomyocarditis Virus Infection
Authors: LE Bazzone, M King, CR MacKay, PP Kyawe, P Meraner, D Lindstrom, J Rojas-Quin, CA Owen, JP Wang, AL Brass, EA Kurt-Jones, RW Finberg
MBio, 2019;10(1):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Enhanced Ability of Oligomeric Nanobodies Targeting MERS Coronavirus Receptor-Binding Domain
Authors: L He, W Tai, J Li, Y Chen, Y Gao, J Li, S Sun, Y Zhou, L Du, G Zhao
Viruses, 2019;11(2):.
Species: Virus
Sample Types:
Applications: Western Blot -
Treatment with XAV-939 prevents in vitro calcification of human valvular interstitial cells
Authors: C Dittfeld, G Reimann, A Mieting, P Büttner, A Jannasch, K Plötze, G Steiner, SM Tugtekin, K Matschke
PLoS ONE, 2018;13(12):e0208774.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo
Authors: R Ibraheim, CQ Song, A Mir, N Amrani, W Xue, EJ Sontheimer
Genome Biol., 2018;19(1):137.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Secondary Antibody -
Talin Modulation by a Synthetic N-Acylurea Derivative Reduces Angiogenesis in Human Endothelial Cells
Authors: IR Lim, HJ Joo, M Jeong, JH Kim, SC Choi, C Kim, JW Jung, SJ Hong
Int J Mol Sci, 2017;18(1):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Re-evaluation of IL-10 signaling reveals novel insights on the contribution of the intracellular domain of the IL-10R2 chain
Authors: RHP Wilbers, DR van Raaij, LB Westerhof, J Bakker, G Smant, A Schots
PLoS ONE, 2017;12(10):e0186317.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity
Authors: MF Gutknecht, ME Seaman, B Ning, DA Cornejo, E Mugler, PF Antkowiak, CA Moskaluk, S Hu, FH Epstein, KA Kelly
Nat Commun, 2017;8(1):552.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Exosomes from embryonic mesenchymal stem cells alleviate osteoarthritis through balancing synthesis and degradation of cartilage extracellular matrix
Authors: Y Wang, D Yu, Z Liu, F Zhou, J Dai, B Wu, J Zhou, BC Heng, XH Zou, H Ouyang, H Liu
Stem Cell Res Ther, 2017;8(1):189.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Rebalancing gene haploinsufficiency in vivo by targeting chromatin
Authors: Filomena Gabriella Fulcoli
Nat Commun, 2016;7(0):11688.
Species: Mouse
Sample Types: Whole Cells
Applications: Western Blot -
Involvement of a C-terminal motif in the interference of primate lentiviral Vpu proteins with CD1d-mediated antigen presentation.
Authors: Bachle S, Sauter D, Sibitz S, Sandberg J, Kirchhoff F, Moll M
Sci Rep, 2015;5(0):9675.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay -
Oxidative stress induces mitochondrial dysfunction in a subset of autism lymphoblastoid cell lines in a well-matched case control cohort.
Authors: Rose, Shannon, Frye, Richard, Slattery, John, Wynne, Rebecca, Tippett, Marie, Pavliv, Oleksand, Melnyk, Stepan, James, S Jill
PLoS ONE, 2014;9(1):e85436.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cleavage of Histone 3 by Cathepsin D in the involuting mammary gland.
Authors: Khalkhali-Ellis, Zhila, Goossens, William, Margaryan, Naira V, Hendrix, Mary J C
PLoS ONE, 2014;9(7):e103230.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas.
Authors: Shahrabi-Farahani, Shokoufe, Wang, Lili, Zwaans, Bernadet, Santana, Jeans M, Shimizu, Akio, Takashima, Seiji, Kreuter, Michael, Coultas, Leigh, D'Amore, Patricia, Arbeit, Jeffrey, Akslen, Lars A, Bielenberg, Diane R
Lab Invest, 2014;94(7):752-65.
Species: Mouse
Sample Types: Whole Cells
Applications: Western Blot -
Nrf2-inducing anti-oxidation stress response in the rat liver--new beneficial effect of lansoprazole.
Authors: Yamashita, Yasunobu, Ueyama, Takashi, Nishi, Toshio, Yamamoto, Yuta, Kawakoshi, Akatsuki, Sunami, Shogo, Iguchi, Mikitaka, Tamai, Hideyuki, Ueda, Kazuki, Ito, Takao, Tsuruo, Yoshihir, Ichinose, Masao
PLoS ONE, 2014;9(5):e97419.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Galectin-3 negatively regulates dendritic cell production of IL-23/IL-17-axis cytokines in infection by Histoplasma capsulatum.
Authors: Wu S, Yu J, Liu F, Miaw S, Wu-Hsieh B
J Immunol, 2013;190(7):3427-37.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Lysophosphatidic Acid Enhances Vascular Endothelial Growth Factor-C Expression in Human Prostate Cancer PC-3 Cells.
Authors: Lin CE, Chen SU, Lin CC
PLoS ONE, 2012;7(7):e41096.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression.
Authors: Furukawa K, Sato T, Katsuno T, Nakagawa T, Noguchi Y, Tokumasa A, Yokote K, Yokosuka O, Saito Y
Biochem. Biophys. Res. Commun., 2011;405(4):521-6.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Soluble HIV Tat protein removes the IL-7 receptor alpha-chain from the surface of resting CD8 T cells and targets it for degradation.
Authors: Faller EM, Sugden SM, McVey MJ
J. Immunol., 2010;185(5):2854-66.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation.
Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D
PLoS ONE, 2010;5(9):e12995.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Cilostazol inhibits modified low-density lipoprotein uptake and foam cell formation in mouse peritoneal macrophages.
Authors: Okutsu R, Yoshikawa T, Nagasawa M, Hirose Y, Takase H, Mitani K, Okada K, Miyakoda G, Yabuuchi Y
Atherosclerosis, 2009;204(2):405-11.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Contribution of globular death domains and unstructured linkers to MyD88.IRAK-4 heterodimer formation: an explanation for the antagonistic activity of MyD88s.
Authors: Mendoza-Barbera E, Corral-Rodriguez MA, Soares-Schanoski A, Velarde M, Macieira S, Messerschmidt A, Lopez-Collazo E, Fuentes-Prior P
Biochem. Biophys. Res. Commun., 2009;380(1):183-7.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells.
Authors: Eckstein N, Servan K, Hildebrandt B, Politz A, von Jonquieres G, Wolf-Kummeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer HD
Cancer Res., 2009;69(7):2996-3003.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Evaluation of an LC8-binding peptide for the attachment of artificial cargo to dynein.
Authors: Bergen JM, Pun SH,
Mol. Pharm., 2007;4(1):119-28.
Species: Human
Sample Types: Antibody
Applications: ELISA Development -
Plasma storage at -80 degrees C does not protect matrix metalloproteinase-9 from degradation.
Authors: Rouy D, Ernens I, Jeanty C, Wagner DR
Anal. Biochem., 2005;338(2):294-8.
Species: Human
Sample Types: Plasma
Applications: Western Blot
FAQs
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Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
- All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
Secondary Antibodies
Reviews for Goat IgG HRP-conjugated Antibody
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Goat IgG HRP-conjugated Antibody
By Anonymous
on 05/01/2018
Application: ELISA
Sample Tested: Serum
Species:
Mouse
