Goat IgG HRP-conjugated Antibody

Catalog # Availability Size / Price Qty
HAF109
Detection of Goat IgGPrimary Antibody by Western Blot
2 Images
Product Details
Citations (20)
FAQs
Supplemental Products
Reviews (1)

Goat IgG HRP-conjugated Antibody Summary

Species Reactivity
Goat
Specificity
Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with human IgG, rabbit IgG, mouse IgG and less than 1% cross-reactivity with chicken IgY is observed.
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Goat IgG
Formulation
Supplied as a 0.2 um filtered solution in PBS with stabilizers.
Label
HRP (Horseradish Peroxidase)

Applications

Recommended Concentration
Sample
Western Blot
Use at a 1:1000 dilution.
 
Simple Western
Use at a 1:50 dilution.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Goat IgGPrimary Antibody by Western Blot View Larger

Detection of Goat IgGPrimary Antibody by Western Blot Western blot shows detection of Goat Anti-Human/Mouse/Rat GRP75/HSPA9BPolyclonal Antibody (Catalog # AF3584)using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody(Catalog # HAF109).  This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 2.

Simple Western Detection of Goat IgG Primary Antibody by Simple WesternTM. View Larger

Detection of Goat IgG Primary Antibody by Simple WesternTM. Simple Western lane view shows detection of Goat Anti-Human/Mouse/Rat TC-PTP Polyclonal Antibody (Catalog #AF1930) using a 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody
(Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Citations for Goat IgG HRP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Re-evaluation of IL-10 signaling reveals novel insights on the contribution of the intracellular domain of the IL-10R2 chain
    Authors: RHP Wilbers, DR van Raaij, LB Westerhof, J Bakker, G Smant, A Schots
    PLoS ONE, 2017;12(10):e0186317.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  2. Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity
    Authors: MF Gutknecht, ME Seaman, B Ning, DA Cornejo, E Mugler, PF Antkowiak, CA Moskaluk, S Hu, FH Epstein, KA Kelly
    Nat Commun, 2017;8(1):552.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  3. Exosomes from embryonic mesenchymal stem cells alleviate osteoarthritis through balancing synthesis and degradation of cartilage extracellular matrix
    Authors: Y Wang, D Yu, Z Liu, F Zhou, J Dai, B Wu, J Zhou, BC Heng, XH Zou, H Ouyang, H Liu
    Stem Cell Res Ther, 2017;8(1):189.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  4. Talin Modulation by a Synthetic N-Acylurea Derivative Reduces Angiogenesis in Human Endothelial Cells
    Authors: IR Lim, HJ Joo, M Jeong, JH Kim, SC Choi, C Kim, JW Jung, SJ Hong
    Int J Mol Sci, 2017;18(1):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  5. Rebalancing gene haploinsufficiency in vivo by targeting chromatin
    Authors: Filomena Gabriella Fulcoli
    Nat Commun, 2016;7(0):11688.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: WB
  6. Involvement of a C-terminal motif in the interference of primate lentiviral Vpu proteins with CD1d-mediated antigen presentation.
    Authors: Bachle S, Sauter D, Sibitz S, Sandberg J, Kirchhoff F, Moll M
    Sci Rep, 2015;5(0):9675.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  7. Cleavage of Histone 3 by Cathepsin D in the involuting mammary gland.
    Authors: Khalkhali-Ellis, Zhila, Goossens, William, Margaryan, Naira V, Hendrix, Mary J C
    PLoS ONE, 2014;9(7):e103230.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  8. Oxidative stress induces mitochondrial dysfunction in a subset of autism lymphoblastoid cell lines in a well-matched case control cohort.
    Authors: Rose, Shannon, Frye, Richard, Slattery, John, Wynne, Rebecca, Tippett, Marie, Pavliv, Oleksand, Melnyk, Stepan, James, S Jill
    PLoS ONE, 2014;9(1):e85436.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  9. Nrf2-inducing anti-oxidation stress response in the rat liver--new beneficial effect of lansoprazole.
    Authors: Yamashita, Yasunobu, Ueyama, Takashi, Nishi, Toshio, Yamamoto, Yuta, Kawakoshi, Akatsuki, Sunami, Shogo, Iguchi, Mikitaka, Tamai, Hideyuki, Ueda, Kazuki, Ito, Takao, Tsuruo, Yoshihir, Ichinose, Masao
    PLoS ONE, 2014;9(5):e97419.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: WB
  10. Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas.
    Authors: Shahrabi-Farahani, Shokoufe, Wang, Lili, Zwaans, Bernadet, Santana, Jeans M, Shimizu, Akio, Takashima, Seiji, Kreuter, Michael, Coultas, Leigh, D'Amore, Patricia, Arbeit, Jeffrey, Akslen, Lars A, Bielenberg, Diane R
    Lab Invest, 2014;94(7):752-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: WB
  11. Galectin-3 negatively regulates dendritic cell production of IL-23/IL-17-axis cytokines in infection by Histoplasma capsulatum.
    Authors: Wu S, Yu J, Liu F, Miaw S, Wu-Hsieh B
    J Immunol, 2013;190(7):3427-37.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  12. Lysophosphatidic Acid Enhances Vascular Endothelial Growth Factor-C Expression in Human Prostate Cancer PC-3 Cells.
    Authors: Lin CE, Chen SU, Lin CC
    PLoS ONE, 2012;7(7):e41096.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  13. Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression.
    Authors: Furukawa K, Sato T, Katsuno T, Nakagawa T, Noguchi Y, Tokumasa A, Yokote K, Yokosuka O, Saito Y
    Biochem. Biophys. Res. Commun., 2011;405(4):521-6.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  14. BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation.
    Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D
    PLoS ONE, 2010;5(9):e12995.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  15. Soluble HIV Tat protein removes the IL-7 receptor alpha-chain from the surface of resting CD8 T cells and targets it for degradation.
    Authors: Faller EM, Sugden SM, McVey MJ
    J. Immunol., 2010;185(5):2854-66.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  16. Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells.
    Authors: Eckstein N, Servan K, Hildebrandt B, Politz A, von Jonquieres G, Wolf-Kummeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer HD
    Cancer Res., 2009;69(7):2996-3003.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  17. Contribution of globular death domains and unstructured linkers to MyD88.IRAK-4 heterodimer formation: an explanation for the antagonistic activity of MyD88s.
    Authors: Mendoza-Barbera E, Corral-Rodriguez MA, Soares-Schanoski A, Velarde M, Macieira S, Messerschmidt A, Lopez-Collazo E, Fuentes-Prior P
    Biochem. Biophys. Res. Commun., 2009;380(1):183-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  18. Cilostazol inhibits modified low-density lipoprotein uptake and foam cell formation in mouse peritoneal macrophages.
    Authors: Okutsu R, Yoshikawa T, Nagasawa M, Hirose Y, Takase H, Mitani K, Okada K, Miyakoda G, Yabuuchi Y
    Atherosclerosis, 2009;204(2):405-11.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  19. Evaluation of an LC8-binding peptide for the attachment of artificial cargo to dynein.
    Authors: Bergen JM, Pun SH,
    Mol. Pharm., 2007;4(1):119-28.
    Species: Human
    Sample Types: Antibody
    Applications: ELISA Development
  20. Plasma storage at -80 degrees C does not protect matrix metalloproteinase-9 from degradation.
    Authors: Rouy D, Ernens I, Jeanty C, Wagner DR
    Anal. Biochem., 2005;338(2):294-8.
    Species: Human
    Sample Types: Plasma
    Applications: WB

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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Reviews for Goat IgG HRP-conjugated Antibody

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Goat IgG HRP-conjugated Antibody
By Anonymous on 05/01/2018
Application: ELISA Sample Tested: Serum Species: Mouse