Goat IgG HRP-conjugated Antibody

Name: Goat IgG HRP-conjugated Antibody
Brand: R&D Systems
Rating: 5 (1 reviews)

HAF109 22 Citations 1 Review Datasheet / COA / SDS

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HAF109

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Detection of Goat IgGPrimary Antibody by Western Blot

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Citations (22)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Goat IgG HRP-conjugated Antibody Summary

Species Reactivity

Goat

Specificity

Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with human IgG, rabbit IgG, mouse IgG and less than 1% cross-reactivity with chicken IgY is observed.

Source

Polyclonal Donkey IgG

Purification

Antigen Affinity-purified

Immunogen

Goat IgG

Formulation

Supplied as a 0.2 um filtered solution in PBS with stabilizers.

Label

HRP (Horseradish Peroxidase)

Applications

Recommended Concentration

Sample

Western Blot

Use at a 1:1000 dilution.

Simple Western

Use at a 1:50 dilution.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot

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Detection of Goat IgGPrimary Antibody by Western Blot Western blot shows detection of Goat Anti-Human/Mouse/Rat GRP75/HSPA9BPolyclonal Antibody (Catalog # AF3584)using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody(Catalog # HAF109). This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 2.

Simple Western

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Detection of Goat IgG Primary Antibody by Simple Western^TM. Simple Western lane view shows detection of Goat Anti-Human/Mouse/Rat TC-PTP Polyclonal Antibody (Catalog #AF1930) using a 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody
(Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Product Datasheet

COA

SDS

Citations for Goat IgG HRP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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Treatment with XAV-939 prevents in vitro calcification of human valvular interstitial cells
Authors: C Dittfeld, G Reimann, A Mieting, P Büttner, A Jannasch, K Plötze, G Steiner, SM Tugtekin, K Matschke
PLoS ONE, 2018;13(12):e0208774.
Species: Human
Sample Types: Cell Lysates
Applications: WB
All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo
Authors: R Ibraheim, CQ Song, A Mir, N Amrani, W Xue, EJ Sontheimer
Genome Biol., 2018;19(1):137.
Species: Mouse
Sample Types: Tissue Homogenate
Applications: WB Secondary
Talin Modulation by a Synthetic N-Acylurea Derivative Reduces Angiogenesis in Human Endothelial Cells
Authors: IR Lim, HJ Joo, M Jeong, JH Kim, SC Choi, C Kim, JW Jung, SJ Hong
Int J Mol Sci, 2017;18(1):.
Species: Human
Sample Types: Cell Lysates
Applications: WB
Re-evaluation of IL-10 signaling reveals novel insights on the contribution of the intracellular domain of the IL-10R2 chain
Authors: RHP Wilbers, DR van Raaij, LB Westerhof, J Bakker, G Smant, A Schots
PLoS ONE, 2017;12(10):e0186317.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow
Exosomes from embryonic mesenchymal stem cells alleviate osteoarthritis through balancing synthesis and degradation of cartilage extracellular matrix
Authors: Y Wang, D Yu, Z Liu, F Zhou, J Dai, B Wu, J Zhou, BC Heng, XH Zou, H Ouyang, H Liu
Stem Cell Res Ther, 2017;8(1):189.
Species: Mouse
Sample Types: Cell Lysates
Applications: WB
Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity
Authors: MF Gutknecht, ME Seaman, B Ning, DA Cornejo, E Mugler, PF Antkowiak, CA Moskaluk, S Hu, FH Epstein, KA Kelly
Nat Commun, 2017;8(1):552.
Species: Human
Sample Types: Cell Lysates
Applications: WB
Rebalancing gene haploinsufficiency in vivo by targeting chromatin
Authors: Filomena Gabriella Fulcoli
Nat Commun, 2016;7(0):11688.
Species: Mouse
Sample Types: Whole Cells
Applications: WB
Involvement of a C-terminal motif in the interference of primate lentiviral Vpu proteins with CD1d-mediated antigen presentation.
Authors: Bachle S, Sauter D, Sibitz S, Sandberg J, Kirchhoff F, Moll M
Sci Rep, 2015;5(0):9675.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay
Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas.
Authors: Shahrabi-Farahani, Shokoufe, Wang, Lili, Zwaans, Bernadet, Santana, Jeans M, Shimizu, Akio, Takashima, Seiji, Kreuter, Michael, Coultas, Leigh, D'Amore, Patricia, Arbeit, Jeffrey, Akslen, Lars A, Bielenberg, Diane R
Lab Invest, 2014;94(7):752-65.
Species: Mouse
Sample Types: Whole Cells
Applications: WB
Nrf2-inducing anti-oxidation stress response in the rat liver--new beneficial effect of lansoprazole.
Authors: Yamashita, Yasunobu, Ueyama, Takashi, Nishi, Toshio, Yamamoto, Yuta, Kawakoshi, Akatsuki, Sunami, Shogo, Iguchi, Mikitaka, Tamai, Hideyuki, Ueda, Kazuki, Ito, Takao, Tsuruo, Yoshihir, Ichinose, Masao
PLoS ONE, 2014;9(5):e97419.
Species: Rat
Sample Types: Tissue Homogenates
Applications: WB

Oxidative stress induces mitochondrial dysfunction in a subset of autism lymphoblastoid cell lines in a well-matched case control cohort.
Authors: Rose, Shannon, Frye, Richard, Slattery, John, Wynne, Rebecca, Tippett, Marie, Pavliv, Oleksand, Melnyk, Stepan, James, S Jill
PLoS ONE, 2014;9(1):e85436.
Species: Human
Sample Types: Cell Lysates
Applications: WB

Cleavage of Histone 3 by Cathepsin D in the involuting mammary gland.
Authors: Khalkhali-Ellis, Zhila, Goossens, William, Margaryan, Naira V, Hendrix, Mary J C
PLoS ONE, 2014;9(7):e103230.
Species: Human
Sample Types: Whole Tissue
Applications: IHC Frozen

Galectin-3 negatively regulates dendritic cell production of IL-23/IL-17-axis cytokines in infection by Histoplasma capsulatum.
Authors: Wu S, Yu J, Liu F, Miaw S, Wu-Hsieh B
J Immunol, 2013;190(7):3427-37.
Species: Mouse
Sample Types: Cell Lysates
Applications: WB

Lysophosphatidic Acid Enhances Vascular Endothelial Growth Factor-C Expression in Human Prostate Cancer PC-3 Cells.
Authors: Lin CE, Chen SU, Lin CC
PLoS ONE, 2012;7(7):e41096.
Species: Human
Sample Types: Cell Lysates
Applications: WB

Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression.
Authors: Furukawa K, Sato T, Katsuno T, Nakagawa T, Noguchi Y, Tokumasa A, Yokote K, Yokosuka O, Saito Y
Biochem. Biophys. Res. Commun., 2011;405(4):521-6.
Species: Mouse
Sample Types: Cell Lysates
Applications: WB

Soluble HIV Tat protein removes the IL-7 receptor alpha-chain from the surface of resting CD8 T cells and targets it for degradation.
Authors: Faller EM, Sugden SM, McVey MJ
J. Immunol., 2010;185(5):2854-66.
Species: Human
Sample Types: Cell Lysates
Applications: WB

BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation.
Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D
PLoS ONE, 2010;5(9):e12995.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: WB

Contribution of globular death domains and unstructured linkers to MyD88.IRAK-4 heterodimer formation: an explanation for the antagonistic activity of MyD88s.
Authors: Mendoza-Barbera E, Corral-Rodriguez MA, Soares-Schanoski A, Velarde M, Macieira S, Messerschmidt A, Lopez-Collazo E, Fuentes-Prior P
Biochem. Biophys. Res. Commun., 2009;380(1):183-7.
Species: Human
Sample Types: Cell Lysates
Applications: WB

Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells.
Authors: Eckstein N, Servan K, Hildebrandt B, Politz A, von Jonquieres G, Wolf-Kummeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer HD
Cancer Res., 2009;69(7):2996-3003.
Species: Human
Sample Types: Cell Lysates
Applications: WB

Cilostazol inhibits modified low-density lipoprotein uptake and foam cell formation in mouse peritoneal macrophages.
Authors: Okutsu R, Yoshikawa T, Nagasawa M, Hirose Y, Takase H, Mitani K, Okada K, Miyakoda G, Yabuuchi Y
Atherosclerosis, 2009;204(2):405-11.
Species: Mouse
Sample Types: Cell Lysates
Applications: WB

Evaluation of an LC8-binding peptide for the attachment of artificial cargo to dynein.
Authors: Bergen JM, Pun SH,
Mol. Pharm., 2007;4(1):119-28.
Species: Human
Sample Types: Antibody
Applications: ELISA Development

Plasma storage at -80 degrees C does not protect matrix metalloproteinase-9 from degradation.
Authors: Rouy D, Ernens I, Jeanty C, Wagner DR
Anal. Biochem., 2005;338(2):294-8.
Species: Human
Sample Types: Plasma
Applications: WB

FAQs

Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
- All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.

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