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Goat IgG HRP-conjugated Antibody

  (20 citations)
(1 Review)     
Datasheet / CoA / SDS
Detection of Goat IgGPrimary Antibody by Western Blot

Western Blot

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Detection of Goat IgG Primary Antibody by Simple Western<sup>TM</sup>.

Simple Western

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Product Details
Citations (20)
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Goat
  • Specificity
    Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with human IgG, rabbit IgG, mouse IgG and less than 1% cross-reactivity with chicken IgY is observed.
  • Source
    Polyclonal Donkey IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Goat IgG
  • Formulation
    Supplied as a 0.2 um filtered solution in PBS with stabilizers.
  • Label
    HRP (Horseradish Peroxidase)
Product Datasheets
Product Datasheet

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    Use at a 1:1000 dilution.
  • Simple Western
    Use at a 1:50 dilution.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Western Blot
Western Blot Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)]
click image to view larger
Detection of Goat IgGPrimary Antibody by Western Blot Western blot shows detection of Goat Anti-Human/Mouse/Rat GRP75/HSPA9BPolyclonal Antibody (Catalog # AF3584)using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody(Catalog # HAF109).  This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 2.
Simple Western
Simple Western Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)]
click image to view larger
Detection of Goat IgG Primary Antibody by Simple WesternTM. Simple Western lane view shows detection of Goat Anti-Human/Mouse/Rat TC-PTP Polyclonal Antibody (Catalog #AF1930) using a 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody
(Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Do not freeze.
    • 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
Filter your results:

Species
 Applications
 Sample Type
  1. Re-evaluation of IL-10 signaling reveals novel insights on the contribution of the intracellular domain of the IL-10R2 chain
    Authors: RHP Wilbers, DR van Raaij, LB Westerhof, J Bakker, G Smant, A Schots
    PLoS ONE, 2017;12(10):e0186317.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity
    Authors: MF Gutknecht, ME Seaman, B Ning, DA Cornejo, E Mugler, PF Antkowiak, CA Moskaluk, S Hu, FH Epstein, KA Kelly
    Nat Commun, 2017;8(1):552.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Exosomes from embryonic mesenchymal stem cells alleviate osteoarthritis through balancing synthesis and degradation of cartilage extracellular matrix
    Authors: Y Wang, D Yu, Z Liu, F Zhou, J Dai, B Wu, J Zhou, BC Heng, XH Zou, H Ouyang, H Liu
    Stem Cell Res Ther, 2017;8(1):189.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Talin Modulation by a Synthetic N-Acylurea Derivative Reduces Angiogenesis in Human Endothelial Cells
    Authors: IR Lim, HJ Joo, M Jeong, JH Kim, SC Choi, C Kim, JW Jung, SJ Hong
    Int J Mol Sci, 2017;18(1):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Rebalancing gene haploinsufficiency in vivo by targeting chromatin
    Authors: Filomena Gabriella Fulcoli
    Nat Commun, 2016;7(0):11688.
    Species: Mouse
    Sample Type: Whole Cells
    Application: WB
  6. Involvement of a C-terminal motif in the interference of primate lentiviral Vpu proteins with CD1d-mediated antigen presentation.
    Authors: Bachle S, Sauter D, Sibitz S, Sandberg J, Kirchhoff F, Moll M
    Sci Rep, 2015;5(0):9675.
    Species: Human
    Sample Type: Cell Lysates
    Application: Bioassay
  7. Oxidative stress induces mitochondrial dysfunction in a subset of autism lymphoblastoid cell lines in a well-matched case control cohort.
    Authors: Rose, Shannon, Frye, Richard, Slattery, John, Wynne, Rebecca, Tippett, Marie, Pavliv, Oleksand, Melnyk, Stepan, James, S Jill
    PLoS ONE, 2014;9(1):e85436.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. Nrf2-inducing anti-oxidation stress response in the rat liver--new beneficial effect of lansoprazole.
    Authors: Yamashita, Yasunobu, Ueyama, Takashi, Nishi, Toshio, Yamamoto, Yuta, Kawakoshi, Akatsuki, Sunami, Shogo, Iguchi, Mikitaka, Tamai, Hideyuki, Ueda, Kazuki, Ito, Takao, Tsuruo, Yoshihir, Ichinose, Masao
    PLoS ONE, 2014;9(5):e97419.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
  9. Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas.
    Authors: Shahrabi-Farahani, Shokoufe, Wang, Lili, Zwaans, Bernadet, Santana, Jeans M, Shimizu, Akio, Takashima, Seiji, Kreuter, Michael, Coultas, Leigh, D'Amore, Patricia, Arbeit, Jeffrey, Akslen, Lars A, Bielenberg, Diane R
    Lab Invest, 2014;94(7):752-65.
    Species: Mouse
    Sample Type: Whole Cells
    Application: WB
  10. Cleavage of Histone 3 by Cathepsin D in the involuting mammary gland.
    Authors: Khalkhali-Ellis, Zhila, Goossens, William, Margaryan, Naira V, Hendrix, Mary J C
    PLoS ONE, 2014;9(7):e103230.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  11. Galectin-3 negatively regulates dendritic cell production of IL-23/IL-17-axis cytokines in infection by Histoplasma capsulatum.
    Authors: Wu S, Yu J, Liu F, Miaw S, Wu-Hsieh B
    J Immunol, 2013;190(7):3427-37.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  12. Lysophosphatidic Acid Enhances Vascular Endothelial Growth Factor-C Expression in Human Prostate Cancer PC-3 Cells.
    Authors: Lin CE, Chen SU, Lin CC
    PLoS ONE, 2012;7(7):e41096.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  13. Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression.
    Authors: Furukawa K, Sato T, Katsuno T, Nakagawa T, Noguchi Y, Tokumasa A, Yokote K, Yokosuka O, Saito Y
    Biochem. Biophys. Res. Commun., 2011;405(4):521-6.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  14. Soluble HIV Tat protein removes the IL-7 receptor alpha-chain from the surface of resting CD8 T cells and targets it for degradation.
    Authors: Faller EM, Sugden SM, McVey MJ
    J. Immunol., 2010;185(5):2854-66.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  15. BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation.
    Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D
    PLoS ONE, 2010;5(9):e12995.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  16. Cilostazol inhibits modified low-density lipoprotein uptake and foam cell formation in mouse peritoneal macrophages.
    Authors: Okutsu R, Yoshikawa T, Nagasawa M, Hirose Y, Takase H, Mitani K, Okada K, Miyakoda G, Yabuuchi Y
    Atherosclerosis, 2009;204(2):405-11.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  17. Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells.
    Authors: Eckstein N, Servan K, Hildebrandt B, Politz A, von Jonquieres G, Wolf-Kummeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer HD
    Cancer Res., 2009;69(7):2996-3003.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  18. Contribution of globular death domains and unstructured linkers to MyD88.IRAK-4 heterodimer formation: an explanation for the antagonistic activity of MyD88s.
    Authors: Mendoza-Barbera E, Corral-Rodriguez MA, Soares-Schanoski A, Velarde M, Macieira S, Messerschmidt A, Lopez-Collazo E, Fuentes-Prior P
    Biochem. Biophys. Res. Commun., 2009;380(1):183-7.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  19. Evaluation of an LC8-binding peptide for the attachment of artificial cargo to dynein.
    Authors: Bergen JM, Pun SH,
    Mol. Pharm., 2007;4(1):119-28.
    Species: Human
    Sample Type: Antibody
    Application: ELISA Development
  20. Plasma storage at -80 degrees C does not protect matrix metalloproteinase-9 from degradation.
    Authors: Rouy D, Ernens I, Jeanty C, Wagner DR
    Anal. Biochem., 2005;338(2):294-8.
    Species: Human
    Sample Type: Plasma
    Application: WB
Expand to show all Citations

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

 WB HAF017 16  
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Goat IgG (H+L) PE-conjugated Antibody

 Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

 Flow , IHC , ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

 Flow , IHC , ICC NL003 6
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Goat IgG Horseradish Peroxidase-conjugated Antibody

 WB HAF019 7  
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Goat IgG (H+L) APC-conjugated Antibody

 Flow F0108 6  
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Donkey Anti-Goat IgG Antibody

 WB AF109 6
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Goat IgG VisUCyte HRP Polymer

 IHC VC004  
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Donkey Anti-Goat IgG Biotinylated Antibody

 WB BAF109 3
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Goat IgG (H+L) Fluorescein-conjugated Antibody

 Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

 Flow , IHC , ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

 WB , ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

 WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

 WB BAF019
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

 Flow F0124
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: ELISA.

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Excellent
  ELISA Mouse Anonymous 05/01/2018
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Summary

Application ELISA
Sample Tested Serum
Species Mouse

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Goat IgG Horseradish Peroxidase-conjugated Antibody
 
Goat IgG (H+L) APC-conjugated Antibody
 
Donkey Anti-Goat IgG Antibody
 
Goat IgG VisUCyte HRP Polymer
 
Donkey Anti-Goat IgG Biotinylated Antibody
 
Goat IgG (H+L) Fluorescein-conjugated Antibody
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