Goat IgG HRP-conjugated Antibody Summary
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data

Detection of Goat IgG Primary Antibody by Western Blot Western blot shows detection of Goat Anti-Human/Mouse/Rat GRP75/HSPA9B Polyclonal Antibody (Catalog # AF3584) using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Goat IgG Primary Antibody by Simple WesternTM. Simple Western lane view shows detection of Goat Anti-Human/Mouse/Rat TC-PTP Polyclonal Antibody (Catalog # AF1930) using a 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Preparation and Storage
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Product Datasheets
Citations for Goat IgG HRP-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Role of Presenilin-1 in Aggressive Human Melanoma
Authors: J Sidor, M Gillette, LA Dezi, G Untiveros, L Strizzi
International Journal of Molecular Sciences, 2022;23(9):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Neutrophil Extracellular Traps Mediate Acute Liver Failure in Regulation of miR-223/neutrophil Elastase Signaling in Mice
Authors: D Ye, J Yao, W Du, C Chen, Y Yang, K Yan, J Li, Y Xu, S Zang, Y Zhang, X Rong, R Zhang, A Xu, J Guo
Cellular and Molecular Gastroenterology and Hepatology, 2022;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Extracellular sialyltransferase st6gal1 in breast tumor cell growth and invasiveness
Authors: NC Hait, A Maiti, R Wu, VL Andersen, CC Hsu, Y Wu, DG Chapla, K Takabe, ME Rusiniak, W Bshara, J Zhang, KW Moremen, JTY Lau
Cancer Gene Therapy, 2022;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Impairment of the Retinal Endothelial Cell Barrier Induced by Long-Term Treatment with VEGF-A165 No Longer Depends on the Growth Factor's Presence
Authors: HL Deissler, M Rehak, A Wolf
Biomolecules, 2022;12(5):.
Species: Bovine
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Angiotensin II triggers neutrophil extracellular traps release linking thromboinflammation with essential hypertension
Authors: A Chrysantho, E Gkaliagkou, A Lazaridis, S Arelaki, P Pateinakis, M Ntinopoulo, A Mitsios, C Antoniadou, C Argyriou, GS Georgiadis, V Papadopoul, A Giatromano, K Ritis, P Skendros
JCI Insight, 2021;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Detection -
Genome-wide CRISPR screening identifies TMEM106B as a proviral host factor for SARS-CoV-2
Authors: J Baggen, L Persoons, E Vanstreels, S Jansen, D Van Loover, B Boeckx, V Geudens, J De Man, D Jochmans, J Wauters, E Wauters, BM Vanaudenae, D Lambrechts, J Neyts, K Dallmeier, HJ Thibaut, M Jacquemyn, P Maes, D Daelemans
Nature Genetics, 2021;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice
Authors: J Frew, HB Nygaard
Acta neuropathologica communications, 2021;9(1):57.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity
Authors: P Szodoray, TK Andersen, J Heinzelbec, JF Imbery, PC Huszthy, SM Stanford, B Bogen, OB Landsverk, N Bottini, A Tveita, LA Munthe, B Nakken
Cell Reports, 2021;36(6):109525.
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Serum amyloid P component is an essential element of resistance against Aspergillus fumigatus
Authors: A Doni, R Parente, I Laface, E Magrini, C Cunha, FS Colombo, JF Lacerda, A Campos, SN Mapelli, F Petroni, R Porte, T Schorn, A Inforzato, T Mercier, K Lagrou, J Maertens, JD Lambris, B Bottazzi, C Garlanda, M Botto, A Carvalho, A Mantovani
Nature Communications, 2021;12(1):3739.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Targeting Lipocalin-2 in Inflammatory Breast Cancer Cells with Small Interference RNA and Small Molecule Inhibitors
Authors: GS Santiago-S, R Noriega-Ri, E Hernández-, F Valiyeva, B Quiñones-D, ES Villodre, BG Debeb, A Rosado-Alb, PE Vivas-Mejí
International Journal of Molecular Sciences, 2021;22(16):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Lipoprotein(a), an Opsonin, Enhances the Phagocytosis of Nontypeable Haemophilus influenzae by Macrophages
Authors: Z Liu, Y Li, Y Wang, Z Liu, Y Su, Q Ma, R Han
Journal of Immunology Research, 2021;2021(0):2185568.
Species: Bacteria
Sample Types: Whole Cells
Applications: ELISA Detection -
Decreased Colonic Activin Receptor-Like Kinase 1 Disrupts Epithelial Barrier Integrity in Patients with Crohn's disease
Authors: T Toyonaga, EC Steinbach, BP Keith, JB Barrow, MR Schaner, EA Wolber, C Beasley, J Huling, Y Wang, NL Allbritton, N Chaumont, TS Sadiq, MJ Koruda, A Jain, MD Long, EL Barnes, HH Herfarth, KL Isaacs, JJ Hansen, MT Shanahan, R Rahbar, TS Furey, P Sethupathy, SZ Sheikh
Cell Mol Gastroenterol Hepatol, 2020;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
NAFLD Aggravates Septic Shock Due to Inadequate Adrenal Response and 11beta-HSDs Dysregulation in Rats
Authors: HC Huang, MH Tsai, FY Lee, TY Lin, CC Chang, CL Chuang, SJ Hsu, MC Hou, YH Huang
Pharmaceutics, 2020;12(5):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Dynamic regulation of hypoxia-inducible factor-1&alpha activity is essential for normal B cell development
Authors: N Burrows, RJM Bashford-R, VJ Bhute, A Peñalver, JR Ferdinand, BJ Stewart, JEG Smith, M Deobagkar-, G Giudice, TM Connor, A Inaba, L Bergamasch, S Smith, MGB Tran, E Petsalaki, PA Lyons, M Espeli, BJP Huntly, KGC Smith, RJ Cornall, MR Clatworthy, PH Maxwell
Nat. Immunol., 2020;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Adiponectin Stimulates Exosome Release to Enhance Mesenchymal Stem-Cell-Driven Therapy of Heart Failure in Mice
Authors: Y Nakamura, S Kita, Y Tanaka, S Fukuda, Y Obata, T Okita, H Nishida, Y Takahashi, Y Kawachi, Y Tsugawa-Sh, Y Fujishima, H Nishizawa, Y Takakura, S Miyagawa, Y Sawa, N Maeda, I Shimomura
Mol. Ther., 2020;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Anti-tumor effects of mevalonate pathway inhibition in ovarian cancer
Authors: A Göbel, VM Zinna, S Dell'Endic, N Jaschke, JD Kuhlmann, P Wimberger, TD Rachner
BMC Cancer, 2020;20(1):703.
Species: Human
Sample Types: Cell Culture Lysates
Applications: Western Blot -
Thymosin &beta4-Enhancing Therapeutic Efficacy of Human Adipose-Derived Stem Cells in Mouse Ischemic Hindlimb Model
Authors: JH Kim, IR Lim, CY Park, HJ Joo, JM Noh, SC Choi, SJ Hong, DS Lim
Int J Mol Sci, 2020;21(6):.
Species: Human
Sample Types: Cell Lysate
Applications: Western Blot -
Tanshinone IIA reduces secretion of pro?angiogenic factors and inhibits angiogenesis in human colorectal cancer
Authors: L Zhou, H Sui, T Wang, R Jia, Z Zhang, J Fu, Y Feng, N Liu, Q Ji, Y Wang, B Zhang, Q Li, Y Li
Oncol. Rep., 2020;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blots -
Prolonged astrocyte-derived erythropoietin expression attenuates neuronal damage under hypothermic conditions
Authors: K Toriuchi, H Kakita, T Tamura, S Takeshita, Y Yamada, M Aoyama
J Neuroinflammation, 2020;17(1):141.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Combined IMIG and Immune Ig Attenuate Allergic Responses in Beagle Dogs
Authors: RM Gorczynski, T Maqbool, G Hoffmann
J Immunol Res, 2020;2020(0):2061609.
Species: Canine
Sample Types: Serum
Applications: ELISA Development -
Induction of 3-hydroxy-3-methylglutaryl-CoA reductase mediates statin resistance in breast cancer cells
Authors: A Göbel, D Breining, M Rauner, LC Hofbauer, TD Rachner
Cell Death Dis, 2019;10(2):91.
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Influenza H1 Mosaic Hemagglutinin Vaccine Induces Broad Immunity and Protection in Mice
Authors: BN Corder, BL Bullard, JL DeBeaucham, NA Ilyushina, RJ Webby, EA Weaver
Vaccines (Basel), 2019;7(4):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Proteomics analysis of colon cancer progression
Authors: S Saleem, S Tariq, I Aleem, Sadr-Ul Sh, M Tahseen, A Atiq, S Hassan, M Abu Bakar, S Khattak, AA Syed, AH Ahmad, M Hussain, MA Yusuf, C Sutton
Clin Proteomics, 2019;16(0):44.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
A Disintegrin and Metalloproteinase 9 Domain (ADAM9) Is a Major Susceptibility Factor in the Early Stages of Encephalomyocarditis Virus Infection
Authors: LE Bazzone, M King, CR MacKay, PP Kyawe, P Meraner, D Lindstrom, J Rojas-Quin, CA Owen, JP Wang, AL Brass, EA Kurt-Jones, RW Finberg
MBio, 2019;10(1):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Enhanced Ability of Oligomeric Nanobodies Targeting MERS Coronavirus Receptor-Binding Domain
Authors: L He, W Tai, J Li, Y Chen, Y Gao, J Li, S Sun, Y Zhou, L Du, G Zhao
Viruses, 2019;11(2):.
Species: Virus
Sample Types:
Applications: Western Blot -
Treatment with XAV-939 prevents in vitro calcification of human valvular interstitial cells
Authors: C Dittfeld, G Reimann, A Mieting, P Büttner, A Jannasch, K Plötze, G Steiner, SM Tugtekin, K Matschke
PLoS ONE, 2018;13(12):e0208774.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo
Authors: R Ibraheim, CQ Song, A Mir, N Amrani, W Xue, EJ Sontheimer
Genome Biol., 2018;19(1):137.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Secondary Antibody -
Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity
Authors: MF Gutknecht, ME Seaman, B Ning, DA Cornejo, E Mugler, PF Antkowiak, CA Moskaluk, S Hu, FH Epstein, KA Kelly
Nat Commun, 2017;8(1):552.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Re-evaluation of IL-10 signaling reveals novel insights on the contribution of the intracellular domain of the IL-10R2 chain
Authors: RHP Wilbers, DR van Raaij, LB Westerhof, J Bakker, G Smant, A Schots
PLoS ONE, 2017;12(10):e0186317.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Exosomes from embryonic mesenchymal stem cells alleviate osteoarthritis through balancing synthesis and degradation of cartilage extracellular matrix
Authors: Y Wang, D Yu, Z Liu, F Zhou, J Dai, B Wu, J Zhou, BC Heng, XH Zou, H Ouyang, H Liu
Stem Cell Res Ther, 2017;8(1):189.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Talin Modulation by a Synthetic N-Acylurea Derivative Reduces Angiogenesis in Human Endothelial Cells
Authors: IR Lim, HJ Joo, M Jeong, JH Kim, SC Choi, C Kim, JW Jung, SJ Hong
Int J Mol Sci, 2017;18(1):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Rebalancing gene haploinsufficiency in vivo by targeting chromatin
Authors: Filomena Gabriella Fulcoli
Nat Commun, 2016;7(0):11688.
Species: Mouse
Sample Types: Whole Cells
Applications: Western Blot -
Involvement of a C-terminal motif in the interference of primate lentiviral Vpu proteins with CD1d-mediated antigen presentation.
Authors: Bachle S, Sauter D, Sibitz S, Sandberg J, Kirchhoff F, Moll M
Sci Rep, 2015;5(0):9675.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay -
Nrf2-inducing anti-oxidation stress response in the rat liver--new beneficial effect of lansoprazole.
Authors: Yamashita, Yasunobu, Ueyama, Takashi, Nishi, Toshio, Yamamoto, Yuta, Kawakoshi, Akatsuki, Sunami, Shogo, Iguchi, Mikitaka, Tamai, Hideyuki, Ueda, Kazuki, Ito, Takao, Tsuruo, Yoshihir, Ichinose, Masao
PLoS ONE, 2014;9(5):e97419.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Oxidative stress induces mitochondrial dysfunction in a subset of autism lymphoblastoid cell lines in a well-matched case control cohort.
Authors: Rose, Shannon, Frye, Richard, Slattery, John, Wynne, Rebecca, Tippett, Marie, Pavliv, Oleksand, Melnyk, Stepan, James, S Jill
PLoS ONE, 2014;9(1):e85436.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas.
Authors: Shahrabi-Farahani, Shokoufe, Wang, Lili, Zwaans, Bernadet, Santana, Jeans M, Shimizu, Akio, Takashima, Seiji, Kreuter, Michael, Coultas, Leigh, D'Amore, Patricia, Arbeit, Jeffrey, Akslen, Lars A, Bielenberg, Diane R
Lab Invest, 2014;94(7):752-65.
Species: Mouse
Sample Types: Whole Cells
Applications: Western Blot -
Cleavage of Histone 3 by Cathepsin D in the involuting mammary gland.
Authors: Khalkhali-Ellis, Zhila, Goossens, William, Margaryan, Naira V, Hendrix, Mary J C
PLoS ONE, 2014;9(7):e103230.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Galectin-3 negatively regulates dendritic cell production of IL-23/IL-17-axis cytokines in infection by Histoplasma capsulatum.
Authors: Wu S, Yu J, Liu F, Miaw S, Wu-Hsieh B
J Immunol, 2013;190(7):3427-37.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Lysophosphatidic Acid Enhances Vascular Endothelial Growth Factor-C Expression in Human Prostate Cancer PC-3 Cells.
Authors: Lin CE, Chen SU, Lin CC
PLoS ONE, 2012;7(7):e41096.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression.
Authors: Furukawa K, Sato T, Katsuno T, Nakagawa T, Noguchi Y, Tokumasa A, Yokote K, Yokosuka O, Saito Y
Biochem. Biophys. Res. Commun., 2011;405(4):521-6.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Soluble HIV Tat protein removes the IL-7 receptor alpha-chain from the surface of resting CD8 T cells and targets it for degradation.
Authors: Faller EM, Sugden SM, McVey MJ
J. Immunol., 2010;185(5):2854-66.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation.
Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D
PLoS ONE, 2010;5(9):e12995.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Cilostazol inhibits modified low-density lipoprotein uptake and foam cell formation in mouse peritoneal macrophages.
Authors: Okutsu R, Yoshikawa T, Nagasawa M, Hirose Y, Takase H, Mitani K, Okada K, Miyakoda G, Yabuuchi Y
Atherosclerosis, 2009;204(2):405-11.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells.
Authors: Eckstein N, Servan K, Hildebrandt B, Politz A, von Jonquieres G, Wolf-Kummeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer HD
Cancer Res., 2009;69(7):2996-3003.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Contribution of globular death domains and unstructured linkers to MyD88.IRAK-4 heterodimer formation: an explanation for the antagonistic activity of MyD88s.
Authors: Mendoza-Barbera E, Corral-Rodriguez MA, Soares-Schanoski A, Velarde M, Macieira S, Messerschmidt A, Lopez-Collazo E, Fuentes-Prior P
Biochem. Biophys. Res. Commun., 2009;380(1):183-7.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Evaluation of an LC8-binding peptide for the attachment of artificial cargo to dynein.
Authors: Bergen JM, Pun SH,
Mol. Pharm., 2007;4(1):119-28.
Species: Human
Sample Types: Antibody
Applications: ELISA Development -
Plasma storage at -80 degrees C does not protect matrix metalloproteinase-9 from degradation.
Authors: Rouy D, Ernens I, Jeanty C, Wagner DR
Anal. Biochem., 2005;338(2):294-8.
Species: Human
Sample Types: Plasma
Applications: Western Blot
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Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
- All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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used as a secondary antibody on Simple Western