Goat IgG HRP-conjugated Antibody

Name: Goat IgG HRP-conjugated Antibody
Brand: R&D Systems

(20 citations)

Datasheet / CoA / SDS

Western Blot

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Simple Western

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Product Details

Citations (20)

FAQs

Supplemental Products

Reviews

Species Reactivity

Goat
Specificity

Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with human IgG, rabbit IgG, mouse IgG and less than 1% cross-reactivity with chicken IgY is observed.
Source

Polyclonal Donkey IgG
Purification

Antigen Affinity-purified
Immunogen

Goat IgG
Formulation

Supplied as a 0.2 um filtered solution in PBS with stabilizers.
Label

HRP (Horseradish Peroxidase)

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

Use at a 1:1000 dilution.
Simple Western

Use at a 1:50 dilution.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot click image to view larger	Detection of Goat IgGPrimary Antibody by Western Blot Western blot shows detection of Goat Anti-Human/Mouse/Rat GRP75/HSPA9BPolyclonal Antibody (Catalog # AF3584)using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody(Catalog # HAF109). This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 2.
Simple Western click image to view larger	Detection of Goat IgG Primary Antibody by Simple Western^TM. Simple Western lane view shows detection of Goat Anti-Human/Mouse/Rat TC-PTP Polyclonal Antibody (Catalog #AF1930) using a 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
- 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name:

Immunoglobulin G

Alternate Names:

IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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Species	Applications	Sample Type
Re-evaluation of IL-10 signaling reveals novel insights on the contribution of the intracellular domain of the IL-10R2 chain Authors: RHP Wilbers, DR van Raaij, LB Westerhof, J Bakker, G Smant, A Schots PLoS ONE, 2017;12(10):e0186317. Species: Mouse Sample Type: Whole Cells Application: Flow Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity Authors: MF Gutknecht, ME Seaman, B Ning, DA Cornejo, E Mugler, PF Antkowiak, CA Moskaluk, S Hu, FH Epstein, KA Kelly Nat Commun, 2017;8(1):552. Species: Human Sample Type: Cell Lysates Application: WB Exosomes from embryonic mesenchymal stem cells alleviate osteoarthritis through balancing synthesis and degradation of cartilage extracellular matrix Authors: Y Wang, D Yu, Z Liu, F Zhou, J Dai, B Wu, J Zhou, BC Heng, XH Zou, H Ouyang, H Liu Stem Cell Res Ther, 2017;8(1):189. Species: Mouse Sample Type: Cell Lysates Application: WB Talin Modulation by a Synthetic N-Acylurea Derivative Reduces Angiogenesis in Human Endothelial Cells Authors: IR Lim, HJ Joo, M Jeong, JH Kim, SC Choi, C Kim, JW Jung, SJ Hong Int J Mol Sci, 2017;18(1):. Species: Human Sample Type: Cell Lysates Application: WB Rebalancing gene haploinsufficiency in vivo by targeting chromatin Authors: Filomena Gabriella Fulcoli Nat Commun, 2016;7(0):11688. Species: Mouse Sample Type: Whole Cells Application: WB Involvement of a C-terminal motif in the interference of primate lentiviral Vpu proteins with CD1d-mediated antigen presentation. Authors: Bachle S, Sauter D, Sibitz S, Sandberg J, Kirchhoff F, Moll M Sci Rep, 2015;5(0):9675. Species: Human Sample Type: Cell Lysates Application: Bioassay Oxidative stress induces mitochondrial dysfunction in a subset of autism lymphoblastoid cell lines in a well-matched case control cohort. Authors: Rose, Shannon, Frye, Richard, Slattery, John, Wynne, Rebecca, Tippett, Marie, Pavliv, Oleksand, Melnyk, Stepan, James, S Jill PLoS ONE, 2014;9(1):e85436. Species: Human Sample Type: Cell Lysates Application: WB Nrf2-inducing anti-oxidation stress response in the rat liver--new beneficial effect of lansoprazole. Authors: Yamashita, Yasunobu, Ueyama, Takashi, Nishi, Toshio, Yamamoto, Yuta, Kawakoshi, Akatsuki, Sunami, Shogo, Iguchi, Mikitaka, Tamai, Hideyuki, Ueda, Kazuki, Ito, Takao, Tsuruo, Yoshihir, Ichinose, Masao PLoS ONE, 2014;9(5):e97419. Species: Rat Sample Type: Tissue Homogenates Application: WB Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas. Authors: Shahrabi-Farahani, Shokoufe, Wang, Lili, Zwaans, Bernadet, Santana, Jeans M, Shimizu, Akio, Takashima, Seiji, Kreuter, Michael, Coultas, Leigh, D'Amore, Patricia, Arbeit, Jeffrey, Akslen, Lars A, Bielenberg, Diane R Lab Invest, 2014;94(7):752-65. Species: Mouse Sample Type: Whole Cells Application: WB Cleavage of Histone 3 by Cathepsin D in the involuting mammary gland. Authors: Khalkhali-Ellis, Zhila, Goossens, William, Margaryan, Naira V, Hendrix, Mary J C PLoS ONE, 2014;9(7):e103230. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Galectin-3 negatively regulates dendritic cell production of IL-23/IL-17-axis cytokines in infection by Histoplasma capsulatum. Authors: Wu S, Yu J, Liu F, Miaw S, Wu-Hsieh B J Immunol, 2013;190(7):3427-37. Species: Mouse Sample Type: Cell Lysates Application: WB Lysophosphatidic Acid Enhances Vascular Endothelial Growth Factor-C Expression in Human Prostate Cancer PC-3 Cells. Authors: Lin CE, Chen SU, Lin CC PLoS ONE, 2012;7(7):e41096. Species: Human Sample Type: Cell Lysates Application: WB Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression. Authors: Furukawa K, Sato T, Katsuno T, Nakagawa T, Noguchi Y, Tokumasa A, Yokote K, Yokosuka O, Saito Y Biochem. Biophys. Res. Commun., 2011;405(4):521-6. Species: Mouse Sample Type: Cell Lysates Application: WB Soluble HIV Tat protein removes the IL-7 receptor alpha-chain from the surface of resting CD8 T cells and targets it for degradation. Authors: Faller EM, Sugden SM, McVey MJ J. Immunol., 2010;185(5):2854-66. Species: Human Sample Type: Cell Lysates Application: WB BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation. Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D PLoS ONE, 2010;5(9):e12995. Species: Mouse Sample Type: Tissue Homogenates Application: WB Cilostazol inhibits modified low-density lipoprotein uptake and foam cell formation in mouse peritoneal macrophages. Authors: Okutsu R, Yoshikawa T, Nagasawa M, Hirose Y, Takase H, Mitani K, Okada K, Miyakoda G, Yabuuchi Y Atherosclerosis, 2009;204(2):405-11. Species: Mouse Sample Type: Cell Lysates Application: WB Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells. Authors: Eckstein N, Servan K, Hildebrandt B, Politz A, von Jonquieres G, Wolf-Kummeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer HD Cancer Res., 2009;69(7):2996-3003. Species: Human Sample Type: Cell Lysates Application: WB Contribution of globular death domains and unstructured linkers to MyD88.IRAK-4 heterodimer formation: an explanation for the antagonistic activity of MyD88s. Authors: Mendoza-Barbera E, Corral-Rodriguez MA, Soares-Schanoski A, Velarde M, Macieira S, Messerschmidt A, Lopez-Collazo E, Fuentes-Prior P Biochem. Biophys. Res. Commun., 2009;380(1):183-7. Species: Human Sample Type: Cell Lysates Application: WB Evaluation of an LC8-binding peptide for the attachment of artificial cargo to dynein. Authors: Bergen JM, Pun SH, Mol. Pharm., 2007;4(1):119-28. Species: Human Sample Type: Antibody Application: ELISA Development Plasma storage at -80 degrees C does not protect matrix metalloproteinase-9 from degradation. Authors: Rouy D, Ernens I, Jeanty C, Wagner DR Anal. Biochem., 2005;338(2):294-8. Species: Human Sample Type: Plasma Application: WB Expand to show all Citations

FAQs

Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
- All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.

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Secondary Antibodies

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