Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2A Clone # 603917R
View all NIK/MAP3K14 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NIK/MAP3K14
Glu769-Pro947
Accession # Q99558

Specificity

Detects human NIK/MAP3K14 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human NIK/MAP3K14 Antibody

NIK/MAP3K14 in Human Pancreas.

NIK/MAP3K14 was detected in immersion fixed paraffin-embedded sections of human pancrease using Mouse Anti-Human NIK/MAP3K14 Monoclonal Antibody (Catalog # MAB6888R) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in exocrine and endocrine cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human NIK/MAP3K14 Antibody

Application
Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample:  immersion fixed paraffin-embedded sections of human pancreas

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NIK/MAP3K14

NIK (NF kappa B inducing kinase), also called MAP3K14, is a widely expressed 947 amino acid (aa), ~100 kDa cytoplasmic protein of the MAP kinase family. NIK participates in a non-canonical NFkB signaling cascade in which its activity and expression are elevated by TNF and related ligands. NIK activates IKKa/b, which releases NFkB subunits for translocation to the nucleus. Within the region used as an immunogen, human NIK shares 94% and 95% aa sequence identity with mouse and rat NIK, respectively.

Long Name

NF-kappa-beta Inducing Kinase

Alternate Names

FTDCR1B, HSNIK, MAP3K14

Entrez Gene IDs

9020 (Human); 53859 (Mouse); 360640 (Rat)

Gene Symbol

MAP3K14

UniProt

Q99558

Additional NIK/MAP3K14 Products

Product Documents for Human NIK/MAP3K14 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NIK/MAP3K14 Antibody

For research use only

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Protocols

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