Detection of NLRP6/NALP6 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human NLRP6/NALP6 PE‑conjugated Monoclonal Antibody (Catalog # IC9145P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
NLRP6 (Nod-Like Receptor Pyrin domain-containing protein 6), also known as NALP6, PYPAF5 and PAN3, is an 80-85 kDa cytoplasmic member of the NLRP family of proteins. Human NLRP is 892 amino acids (aa) in length, and contains an N-terminal Pyrin domain (aa 1-103), one Nacht region (aa 197-350), and five consecutive C-terminqal leucine-rich repeats (aa 462-868). It is expressed in select cell types, including eosinophils, neutrophils, CD4+ and CD8+ T cells, neurons, myofibroblasts, and intestinal goblet cells plus columnar epithelium. NLRP6 is perhaps best known for its participation in the formation of inflammasomes. Inflammasomes are oligomeric intracellular complexes that serve as detectors for "danger signals" that appear following microbial invasion or unexpected cell death. Following detection, recruited components to the complex (such as Caspase-1) are activated, and subsequently themselves serve to activate pro-IL-1 beta and IL-18. The actual detection of these signals is mediated by the NLRP family, possibly in a tissue specific manner. NLRP6 has been identified in both damaged peripheral nerve and intestinal epithelium. In goblet cells, NLRP6 drives mucus secretion; in intestinal columnar epithelium, NLRP6 acts as a homeostatic agent that promotes epithelial barrier integrity. It accomplishes this by inducing mature IL-18 secretion, a step that then reduces IL-22BP production and leads to an increase in IL-22, a cytokine known for its pro-mucosal homeostatic properties. Although most insults to the intestinal mucosa likely arise from bacteria, NLRP6 is also known to recognize dsRNA-based viruses such as norovirus, and induce the expression of antiviral cytokines such as IFN-alpha, -beta and -lambda. Over aa 35-193, human NLRP6 shares 69% aa sequence identity with mouse NLRP6.
NLR Family, Pyrin Domain Containing 6
Entrez Gene IDs:
171389 (Human); 101613 (Mouse); 171390 (Rat)
Angiotensin II/Vasopressin Receptor; AVR; CLR11.4; NACHT, Leucine Rich Repeat And PYD Containing 6; NACHT, LRR And PYD Containing Protein 6; NACHT, LRR And PYD Domains-Containing Protein 6; NALP6; NAVR; NAVR/AVR; NLR Family, Pyrin Domain Containing 6; NLRP6 ; Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 6; PAN3; PYPAF5; PYRIN-Containing APAF1-Like Protein 5
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