Human NLRP6/NALP6 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human NLRP6/NALP6 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 920631
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E.coli-derived recombinant human NLRP6/NALP6
    Accession # P59044
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of NLRP6/NALP6 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human NLRP6/NALP6 PE‑conjugated Monoclonal Antibody (Catalog # IC9145P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NLRP6/NALP6
NLRP6 (Nod-Like Receptor Pyrin domain-containing protein 6), also known as NALP6, PYPAF5 and PAN3, is an 80-85 kDa cytoplasmic member of the NLRP family of proteins. Human NLRP is 892 amino acids (aa) in length, and contains an N-terminal Pyrin domain (aa 1-103), one Nacht region (aa 197-350), and five consecutive C-terminqal leucine-rich repeats (aa 462-868). It is expressed in select cell types, including eosinophils, neutrophils, CD4+ and CD8+ T cells, neurons, myofibroblasts, and intestinal goblet cells plus columnar epithelium. NLRP6 is perhaps best known for its participation in the formation of inflammasomes. Inflammasomes are oligomeric intracellular complexes that serve as detectors for "danger signals" that appear following microbial invasion or unexpected cell death. Following detection, recruited components to the complex (such as Caspase-1) are activated, and subsequently themselves serve to activate pro-IL-1 beta and IL-18. The actual detection of these signals is mediated by the NLRP family, possibly in a tissue specific manner. NLRP6 has been identified in both damaged peripheral nerve and intestinal epithelium. In goblet cells, NLRP6 drives mucus secretion; in intestinal columnar epithelium, NLRP6 acts as a homeostatic agent that promotes epithelial barrier integrity. It accomplishes this by inducing mature IL-18 secretion, a step that then reduces IL-22BP production and leads to an increase in IL-22, a cytokine known for its pro-mucosal homeostatic properties. Although most insults to the intestinal mucosa likely arise from bacteria, NLRP6 is also known to recognize dsRNA-based viruses such as norovirus, and induce the expression of antiviral cytokines such as IFN-alpha, -beta and -lambda. Over aa 35-193, human NLRP6 shares 69% aa sequence identity with mouse NLRP6.
  • Long Name:
    NLR Family, Pyrin Domain Containing 6
  • Entrez Gene IDs:
    171389 (Human); 101613 (Mouse); 171390 (Rat)
  • Alternate Names:
    Angiotensin II/Vasopressin Receptor; AVR; CLR11.4; NACHT, Leucine Rich Repeat And PYD Containing 6; NACHT, LRR And PYD Containing Protein 6; NACHT, LRR And PYD Domains-Containing Protein 6; NALP6; NAVR; NAVR/AVR; NLR Family, Pyrin Domain Containing 6; NLRP6 ; Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 6; PAN3; PYPAF5; PYRIN-Containing APAF1-Like Protein 5
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 12  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human NLRP6/NALP6 PE-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee


Customer Information