Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 257224
View all Olig 1, 2, 3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Olig1
aa 1-272

Specificity

Detects human Olig1, 2, 3. In direct ELISAs, this antibody shows approximately 20% cross-reactivity with recombinant human (rh) Olig2 and 50-100% cross-reactivity with rhOlig3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Olig1, 2, 3 PE‑conjugated Antibody

Detection of Olig1, 2, 3 antibody in A172 Human Cell Line antibody by Flow Cytometry.

Detection of Olig1, 2, 3 in A172 Human Cell Line by Flow Cytometry.

A172 human glioblastoma cell line was stained with Mouse Anti-Human Olig1, 2, 3 PE-conjugated Monoclonal Antibody (Catalog # IC2230P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Olig1, 2, 3 PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: A172 human glioblastoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Olig 1, 2, 3

Olig1 and Olig2, which have been implicated in oligodendrogenesis, are expressed in the region of the ventral ventricular zone of late embryonic spinal cord where oligodendrocyte progenitors appear. Olig3 is transiently expressed in different types of progenitors of embryonic central nervous system and then disappears in the course of development. In adults, expression of Olig3 is primarily detected in neural tissues.

Long Name

Oligodendrocyte Lineage Gene

Additional Olig 1, 2, 3 Products

Product Documents for Human Olig1, 2, 3 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Olig1, 2, 3 PE‑conjugated Antibody

For research use only

Citations for Human Olig1, 2, 3 PE‑conjugated Antibody

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