Human Olig 1, 2, 3 Antibody

Catalog # Availability Size / Price Qty
MAB2230
MAB2230-SP
Product Details
Citations (1)
FAQs
Supplemental Products
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Human Olig 1, 2, 3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Olig 1, 2, 3. In direct ELISAs, this antibody shows approximately 20% cross‑reactivity with recombinant human (rh) Olig2 and 50 - 100% cross‑reactivity with rhOlig3.
Source
Monoclonal Mouse IgG2A Clone # 257224
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Olig1
aa 1-272
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
Immersion fixed frozen sections of embryonic mouse (E11) spinal cord
Immunocytochemistry
8-25 µg/mL
Immersion fixed A172 human glioblastoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Olig 1, 2, 3

Olig1 and Olig2, which have been implicated in oligodendrogenesis, are expressed in the region of the ventral ventricular zone of late embryonic spinal cord where oligodendrocyte progenitors appear. Olig3 is transiently expressed in different types of progenitors of embryonic central nervous system and then disappears in the course of development. In adult, expression of Olig3 is primarily detected in neural tissues.

Long Name
Oligodendrocyte Lineage Gene
Alternate Names
bHLHb6; bHLHe21; Olig 1, 2, 3

Product Datasheets

Citation for Human Olig 1, 2, 3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Relevance of combinatorial profiles of intermediate filaments and transcription factors for glioma histogenesis.
    Authors: Colin C, Virard I, Baeza N, Tchoghandjian A, Fernandez C, Bouvier C, Calisti A, Tong S, Durbec P, Figarella-Branger D
    Neuropathol. Appl. Neurobiol., 2008;33(4):431-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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