Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunocytochemistry

Cited:

Immunohistochemistry-Paraffin, Bioassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 257224
View all Olig 1, 2, 3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Olig1
aa 1-272

Specificity

Detects human Olig 1, 2, 3. In direct ELISAs, this antibody shows approximately 20% cross‑reactivity with recombinant human (rh) Olig2 and 50 - 100% cross‑reactivity with rhOlig3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Olig 1, 2, 3 Antibody

Olig 1, 2, 3 in Mouse Embryo.

Olig 1, 2, 3 was detected in immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo using Mouse Anti-Human Olig 1, 2, 3 Monoclonal Antibody (Catalog # MAB2230) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in neurons in developing brain. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Olig 1, 2, 3 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A172 human glioblastoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Olig 1, 2, 3

Olig1 and Olig2, which have been implicated in oligodendrogenesis, are expressed in the region of the ventral ventricular zone of late embryonic spinal cord where oligodendrocyte progenitors appear. Olig3 is transiently expressed in different types of progenitors of embryonic central nervous system and then disappears in the course of development. In adult, expression of Olig3 is primarily detected in neural tissues.

Long Name

Oligodendrocyte Lineage Gene

Additional Olig 1, 2, 3 Products

Product Documents for Human Olig 1, 2, 3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Olig 1, 2, 3 Antibody

For research use only

Citations for Human Olig 1, 2, 3 Antibody

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Protocols

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FAQs

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