Human Olig3 Biotinylated Affinity Purified PAb

R&D Systems | Catalog # BAF2356

R&D Systems
Discontinued Product
BAF2356 has been discontinued. View all Olig3 products.

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

E. coli-derived recombinant human Olig3
Met1-Lys272
Accession # Q7RTU3

Specificity

Detects human Olig3 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) Olig1 and rhOlig2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Olig3 Biotinylated Affinity Purified PAb

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse spinal cord (E11)

Western Blot

0.1 µg/mL
Sample: Recombinant Human Olig3

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Olig3

The Olig family is a sub-family of the basic helix-loop-helix transcription factors. Olig1 and Olig2, which have been implicated in oligodendrogenesis, are expressed in the region of the ventral ventricular zone of late embryonic spinal cord where oligodendrocyte progenitors appear. Olig3 is transiently expressed in different types of progenitors of embryonic central nervous system and then disappears in the course of development. In adult, expression of Olig3 is primarily detected in non-neural tissues.

Long Name

Oligodendrocyte Lineage Transcription Factor 3

Alternate Names

BHLHB7

Entrez Gene IDs

167826 (Human); 94222 (Mouse)

Gene Symbol

OLIG3

UniProt

Additional Olig3 Products

Product Documents for Human Olig3 Biotinylated Affinity Purified PAb

Certificate of Analysis

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Product Specific Notices for Human Olig3 Biotinylated Affinity Purified PAb

For research use only

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Protocols

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FAQs

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