Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 9E1
View all P-Selectin/CD62P Primary Antibodies »

Product Specifications

Immunogen

Recombinant human P-Selectin
Extracellular domain

Specificity

This antibody binds to CHO cells transfected with human P-Selectin but not to CHO cells transfected with either human E-Selectin or human L-Selectin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human P‑Selectin/CD62P Fluorescein‑conjugated Antibody

Detection of P‑Selectin/CD62P in Whole blood monocytes cells by Flow Cytometry.

Whole blood monocytes cells were stained with Mouse Anti-Human P‑Selectin/CD62P Fluorescein‑conjugated Monoclonal Antibody (Catalog # BBA34, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human P‑Selectin/CD62P Fluorescein‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Whole blood monocytes cells.

Reviewed Applications

Read 2 reviews rated 4.5 using BBA34 in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: P-Selectin/CD62P

Human P-Selectin, also known as GMP-140, LECAM-3, PADGEM, and CD62P, is a member of the Selectin family, and is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin Glycoprotein Ligand-1).

P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling' of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. ELISA techniques have shown that detectable levels of soluble P-Selectin are present in the biological fluids of apparently normal individuals.

Alternate Names

CD62P, GMP140, GRMP, PADGEM, PSEL, SELP

Entrez Gene IDs

6403 (Human); 20344 (Mouse)

Gene Symbol

SELP

Additional P-Selectin/CD62P Products

Product Documents for Human P‑Selectin/CD62P Fluorescein‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human P‑Selectin/CD62P Fluorescein‑conjugated Antibody

For research use only

Citations for Human P‑Selectin/CD62P Fluorescein‑conjugated Antibody

Customer Reviews for Human P‑Selectin/CD62P Fluorescein‑conjugated Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human P‑Selectin/CD62P Fluorescein‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Human P-Selectin/CD62P Fluorescein-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood cells
    Species: Human
    Verified Customer | Posted 07/07/2017
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 21817096
    Species: Human
    Verified Customer | Posted 01/20/2015

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies