Human P-Selectin/CD62P Fluorescein-conjugated Antibody

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Detection of P‑Selectin/CD62P in Whole blood monocytes cells by Flow Cytometry.
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Citations (5)
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Human P-Selectin/CD62P Fluorescein-conjugated Antibody Summary

Species Reactivity
This antibody binds to CHO cells transfected with human P-Selectin but not to CHO cells transfected with either human E-Selectin or human L-Selectin.
Monoclonal Mouse IgG1 Clone # 9E1
Protein A or G purified from hybridoma culture supernatant
Recombinant human P-Selectin
Extracellular domain
Supplied in a saline solution containing BSA and Sodium Azide.
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of P‑Selectin/CD62P in Whole blood monocytes cells by Flow Cytometry. Whole blood monocytes cells were stained with Mouse Anti-Human P‑Selectin/CD62P Fluorescein‑conjugated Monoclonal Antibody (Catalog # BBA34, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: P-Selectin/CD62P

Human P-Selectin, also known as GMP-140, LECAM-3, PADGEM, and CD62P, is a member of the Selectin family, and is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin Glycoprotein Ligand-1).

P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling' of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. ELISA techniques have shown that detectable levels of soluble P-Selectin are present in the biological fluids of apparently normal individuals.

Entrez Gene IDs
6403 (Human); 20344 (Mouse)
Alternate Names
CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP

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Citations for Human P-Selectin/CD62P Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Inhibitory mechanisms of very low-dose rivaroxaban in non-ST-elevation myocardial infarction
    Authors: O Borst, P Münzer, N Alnaggar, S Geue, R Tegtmeyer, D Rath, M Droppa, P Seizer, S Heitmeier, JWM Heemskerk, LK Jennings, RF Storey, DJ Angiolillo, B Rocca, H Spronk, H Ten Cate, M Gawaz, T Geisler
    Blood Adv, 2018-03-27;2(6):715-730.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Platelet activation determines the severity of thrombocytopenia in dengue infection
    Authors: A Ojha, D Nandi, H Batra, R Singhal, GK Annarapu, S Bhattachar, T Seth, L Dar, GR Medigeshi, S Vrati, NK Vikram, P Guchhait
    Sci Rep, 2017-01-31;7(0):41697.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. HbS Binding to GP1b? Activates Platelets in Sickle Cell Disease
    PLoS ONE, 2016-12-09;11(12):e0167899.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Dehydroepiandrosterone inhibits the TNF-alpha-induced inflammatory response in human umbilical vein endothelial cells.
    Authors: Gutierrez G, Mendoza C, Zapata E, Montiel A, Reyes E, Montano LF, Lopez-Marure R
    Atherosclerosis, 2006-03-30;190(1):90-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.
    Authors: Ramachandran R, Morice AH, Compton SJ
    Am. J. Respir. Cell Mol. Biol., 2006-02-23;35(1):133-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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Human P-Selectin/CD62P Fluorescein-conjugated Antibody
By Anonymous on 07/07/2017
Application: Flow Sample Tested: Peripheral blood cells Species: Human