Human P-Selectin/CD62P Antibody

P-Selectin/CD62P in Human Breast Cancer Tissue. P-Selectin/CD62P was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Human P-Selectin/CD62P Monoclonal Antibody (Catalog # BBA30) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of P-Selectin/CD62P by Immunohistochemistry SERS-BFNP molecular imaging of atherosclerotic coronary arteries. A single human coronary artery was isolated from the heart of a patient undergoing heart transplantation surgery. The lumen of the artery segment was then injected with a mixture of anti-ICAM-1, anti-VCAM-1, anti-P-selectin, and isotype control BFNP, sutured closed, and incubated at 37 °C/5% CO2 for 12 h. Sutures were then removed and the artery segment was thoroughly washed prior to SERS spectroscopy and subsequent analysis of morphology, expression of adhesion molecules and SERS mapping. (B) Immunofluorescence staining for CD31, and expression of (C) ICAM-1, VCAM-1 and P-selectin are shown in red. Nuclei were counterstained using Hoechst 33342 (blue). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30613292), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of P-Selectin/CD62P by Immunocytochemistry/ Immunofluorescence Following stimulation, coronary artery endothelial cells (CAEC) express adhesion molecules detectable via immuno-SERS imaging in single and multiplex formats. (A) Fluorescence images of immunohistochemical staining of ICAM-1, VCAM-1 and P-selectin on CAEC in unstimulated and 10 ng/mL TNF-alpha -stimulated conditions. Isotype control, ICAM-1, VCAM-1 and P-selectin staining shown in green; nuclei were counterstained using Hoechst 33342 (blue). (B) CAEC were stimulated with 10 ng/mL TNF-alpha for 24 h, fixed in acetone, and incubated with isotype control, anti-ICAM-1, anti-VCAM-1 or anti-P-selectin BFNP or (C) with all BFNP simultaneously before being subjected to SERS mapping. (D) Representative spectra from anti-ICAM-1 (purple), anti-VCAM-1 (red) and anti-P-selectin (blue) BFNP acquired from the color-matched circles in (C) are shown above their respective reference spectra. Optical images in (B-C) are darkfield images. Scale bars = 20 μm. Results are representative of 3 independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30613292), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of P-Selectin/CD62P by Immunocytochemistry/ Immunofluorescence KD platelets crosstalk with monocytes via forming “adhesion junctions”. A) Flow cytometry analysis showing CD62p expression in platelets from HS (n = 24) and KD patients (n = 24). Unpaired t test. B) Correlation between MPA and CD62p positive platelets in patients with acute KD (n = 24). p value was calculated using correlation analysis. C) Heatmap showing selected ligand–receptor interactions between MPA and subtypes of monocytes. D) Immunostaining of PSGL‐1 (blue), CD14 (green), CD41 (red), and CD62p (gray) in monocytes after coculture with KD platelets for 8 h. Activated CD41+ platelets (red) forming CD62p (gray) and PSGL‐1‐mediated junctions (blue) with CD14+ monocytes (green) are shown (n = 6). Scale bar: 5 µm. E) Immunostaining of CD11b (blue), CD14 (green), CD41 (red), GPIb alpha (gray) in monocytes after coculture with KD platelets for 8 h. Activated CD41+ platelets (red) forming GPIb alpha (gray) and CD11b‐mediated junctions (blue) with CD14+ monocytes (green) are shown (n = 6). Scale bar: 5 µm. F,G) Proximity ligation assay (PLA) showing adhesion junctions of CD62p–PSGL‐1 (F), GPIb alpha –CD11b (G) on monocytes. PLA staining: red; nuclei: blue; wheat germ agglutinin (WGA): green (n = 5). Scale bar: 10 µm. PLT, platelet; HS, healthy subject; KD, Kawasaki disease; MPA, platelet–monocyte aggregate; ITGAM, integrin subunit alpha M (CD11b). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39665236), licensed under a CC-BY license. Not internally tested by R&D Systems.