Human P-Selectin/CD62P Antibody

Catalog # Availability Size / Price Qty
P-Selectin/CD62P in Human Breast Cancer Tissue.
1 Image
Product Details
Citations (5)
Supplemental Products
Reviews (1)

Human P-Selectin/CD62P Antibody Summary

Species Reactivity
This antibody binds to CHO cells transfected with human P-Selectin but not to CHO cells transfected with either human E-Selectin or human L-Selectin.
Monoclonal Mouse IgG1 Clone # 9E1
Protein A or G purified from hybridoma culture supernatant
Recombinant human P-Selectin
Extracellular domain
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
8-25 µg/mL
See below
Adhesion Blockade
The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human P-Selectin/CD62P (Catalog # ADP3, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 1 µg/mL of the antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry P-Selectin/CD62P antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

P-Selectin/CD62P in Human Breast Cancer Tissue. P-Selectin/CD62P was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Human P-Selectin/CD62P Monoclonal Antibody (Catalog # BBA30) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P-Selectin/CD62P

P-Selectin, also known as GMP-140 and PADGEM, is a transmembrane glycoprotein expressed by activated platelets and endothelial cells. P-Selectin binds to PSGL-1 on myeloid cells, neutrophils, monocytes, and lymphocytes. P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium.

Entrez Gene IDs
6403 (Human); 20344 (Mouse)
Alternate Names
CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP

Product Datasheets

You must select a language.


Citations for Human P-Selectin/CD62P Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

  1. Adhesion of T cells to endothelial cells facilitates blinatumomab-associated neurologic adverse events
    Authors: M Klinger, G Zugmaier, V Nägele, ME Goebeler, C Brandl, M Stelljes, H Lassmann, A von Stacke, RC Bargou, P Kufer
    Cancer Res., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  2. Characterization of platelet-monocyte complexes in HIV-1-infected individuals: possible role in HIV-associated neuroinflammation.
    Authors: Singh, Meera V, Davidson, Donna C, Jackson, Joseph W, Singh, Vir B, Silva, Jharon, Ramirez, Servio H, Maggirwar, Sanjay B
    J Immunol, 2014;192(10):4674-84.
    Species: Human
    Sample Types: Whole Blood
    Applications: Neutralization
  3. Platelet activation and apoptosis modulate monocyte inflammatory responses in dengue.
    Authors: Hottz E, Medeiros-de-Moraes I, Vieira-de-Abreu A, de Assis E, Vals-de-Souza R, Castro-Faria-Neto H, Weyrich A, Zimmerman G, Bozza F, Bozza P
    J Immunol, 2014;193(4):1864-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. A role for the L-selectin adhesion system in mediating cytotrophoblast emigration from the placenta.
    Authors: Prakobphol A, Genbacev O, Gormley M, Kapidzic M, Fisher SJ
    Dev. Biol., 2006;298(1):107-17.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. P-Selectin-mediated acute inflammation can be blocked by chemically modified heparin, RO-heparin.
    Authors: Gao Y, Li N, Fei R, Chen Z, Zheng S, Zeng X
    Mol. Cells, 2005;19(3):350-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human P-Selectin/CD62P Antibody

Average Rating: 4 (Based on 1 Review)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Human P-Selectin/CD62P Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Human P-Selectin/CD62P Antibody
By Balaji Mahender on 12/20/2017
Application: ELISA Sample Tested: Citrate Plasma,EDTA Plasma,Heparin Plasma Species: Human