Human P-Selectin/CD62P Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    This antibody binds to CHO cells transfected with human P-Selectin but not to CHO cells transfected with either human E-Selectin or human L-Selectin.
  • Source
    Monoclonal Mouse IgG1 Clone # 9E1
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Recombinant human P-Selectin
    Extracellular domain
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Adhesion Blockade
    The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human P-Selectin/CD62P (Catalog # ADP3, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 1 µg/mL of the antibody.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
P-Selectin/CD62P in Human Breast Cancer Tissue. P‑Selectin/CD62P was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Human P‑Selectin/CD62P Monoclonal Antibody (Catalog # BBA30) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: P-Selectin/CD62P
P-Selectin, also known as GMP-140 and PADGEM, is a transmembrane glycoprotein expressed by activated platelets and endothelial cells. P-Selectin binds to PSGL-1 on myeloid cells, neutrophils, monocytes, and lymphocytes. P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium.
  • Entrez Gene IDs:
    6403 (Human); 20344 (Mouse)
  • Alternate Names:
    CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Characterization of platelet-monocyte complexes in HIV-1-infected individuals: possible role in HIV-associated neuroinflammation.
    Authors: Singh, Meera V, Davidson, Donna C, Jackson, Joseph W, Singh, Vir B, Silva, Jharon, Ramirez, Servio H, Maggirwar, Sanjay B
    J Immunol, 2014;192(10):4674-84.
    Species: Human
    Sample Type: Whole Blood
    Application: Neut
  2. Platelet activation and apoptosis modulate monocyte inflammatory responses in dengue.
    Authors: Hottz E, Medeiros-de-Moraes I, Vieira-de-Abreu A, de Assis E, Vals-de-Souza R, Castro-Faria-Neto H, Weyrich A, Zimmerman G, Bozza F, Bozza P
    J Immunol, 2014;193(4):1864-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. A role for the L-selectin adhesion system in mediating cytotrophoblast emigration from the placenta.
    Authors: Prakobphol A, Genbacev O, Gormley M, Kapidzic M, Fisher SJ
    Dev. Biol., 2006;298(1):107-17.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. P-Selectin-mediated acute inflammation can be blocked by chemically modified heparin, RO-heparin.
    Authors: Gao Y, Li N, Fei R, Chen Z, Zheng S, Zeng X
    Mol. Cells, 2005;19(3):350-5.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
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