This antibody binds to CHO cells transfected with human P-Selectin but not to CHO cells transfected with either human E-Selectin or human L-Selectin.
Monoclonal Mouse IgG1 Clone # 9E1
Protein A or G purified from hybridoma culture supernatant
Recombinant human P-Selectin Extracellular domain
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
<0.10 EU per 1 μg of the antibody by the LAL method.
The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human P-Selectin/CD62P (Catalog # ADP3, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 1 µg/mL of the antibody.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
P-Selectin/CD62P in Human Breast Cancer Tissue. P‑Selectin/CD62P was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Human P‑Selectin/CD62P Monoclonal Antibody (Catalog # BBA30) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
P-Selectin, also known as GMP-140 and PADGEM, is a transmembrane
glycoprotein expressed by activated platelets and endothelial cells.
P-Selectin binds to PSGL-1 on myeloid cells, neutrophils, monocytes,
and lymphocytes. P-Selectin plays a role in the adhesion of leukocytes
and neutrophils to the endothelium.
Entrez Gene IDs:
6403 (Human); 20344 (Mouse)
CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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