This antibody binds to CHO cells transfected with human P-Selectin but not to CHO cells transfected with either human E-Selectin or human L-Selectin.
Monoclonal Mouse IgG1 Clone # 9E1
Protein A or G purified from hybridoma culture supernatant
Recombinant human P-Selectin Extracellular domain
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
<0.10 EU per 1 μg of the antibody by the LAL method.
The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human P-Selectin/CD62P (Catalog # ADP3, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 1 µg/mL of the antibody.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
P-Selectin/CD62P in Human Breast Cancer Tissue. P‑Selectin/CD62P was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Human P‑Selectin/CD62P Monoclonal Antibody (Catalog # BBA30) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
P-Selectin, also known as GMP-140 and PADGEM, is a transmembrane
glycoprotein expressed by activated platelets and endothelial cells.
P-Selectin binds to PSGL-1 on myeloid cells, neutrophils, monocytes,
and lymphocytes. P-Selectin plays a role in the adhesion of leukocytes
and neutrophils to the endothelium.
Entrez Gene IDs:
6403 (Human); 20344 (Mouse)
CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; selectin P (granule membrane protein 140kDa, antigen CD62); SELP
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.